High-efficiency and rapid micropropagation method for toxicodendron vernicifluum

A small wood paint, fast technology, applied in horticultural methods, botanical equipment and methods, climate change adaptation, etc., can solve the problems of low seedling formation rate and slow reproduction speed, and achieve stable genetic traits, easy rooting, and cultivation process. simple effect

Active Publication Date: 2014-09-03
SOUTHWEST FORESTRY UNIVERSITY
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention solves the problems of low seedling ra

Method used

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Examples

Experimental program
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Embodiment 1

[0016] Example 1. The implementation time was 2013. The tissue culture and propagation of small wood lacquer was carried out in the laboratory of the College of Forestry of Southwest Forestry University and the Greenhouse.

[0017] (1) Selection and disinfection of explants: In May 2013, select a good sumac single plant to obtain its tender stems of the year, remove the petioles and leaves, brush the stem surface with a brush to remove surface impurities, and use 2% Soak in the detergent aqueous solution for 10 minutes, rinse in linear tap water for 2 hours, cut into 3-4cm long stems with 1-2 leaf axils, put them in the sterilized explant tank, and move to ultra-clean work Disinfect inside the station, soak in 75% ethanol for 20 seconds, soak in 1% benzalkonium chloride solution for 1 minute, rinse in sterile distilled water for 2-3 times, soak in 0.1% mercury liters for 2 minutes, and rinse in sterile distilled water for 5-8 Second, put it in the sterilized inoculation tray with...

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Abstract

The invention relates to a plant tissue culture method, in particular to a tissue culture method of toxicodendron vernicifluum, and particularly relates to a high-efficiency and rapid micropropagation method for toxicodendron vernicifluum. The high-efficiency and rapid micropropagation method for toxicodendron vernicifluum comprises the following steps: (1) selecting and disinfecting an explant; (2) starting culture; (3) conducting enrichment culture; (4) conducting rooting culture; and (5) hardening seedlings and transplanting. The toxicodendron vernicifluum has the advantages of being stable in inheritable character, simple in culture process, easy to root, short in culture micropropagation period, and easy to survive after being transplanted. By adopting a method for conducting tissue culture on excellent single plants of toxicodendron vernicifluum, the propagation coefficient can achieve four to five times, the annual propagation amount of every ten effective toxicodendron vernicifluum axillary buds can still achieve more than 1.75 million calculated according to the propagation coefficient of 4.5 and the propagation period of 45 days despite pollution loss, the rapid propagation problem of the toxicodendron vernicifluum can be effectively solved, the excellent clone materials can be popularized on a large area, and foundation is laid for the industrial seedling.

Description

Technical field [0001] The invention relates to a plant tissue culture method, especially a tissue culture method of small wood paint. Background technique [0002] Sumac Toxicodendron vernicifluum ) Is an important economic tree species of the lacquer genus in the Anacardiacceae family. Its main product, "raw lacquer", is the sap from tree trunks. It is currently the only biopolymer coating in the world that is derived from green plants and can be cured into a film at room temperature under the catalysis of biological enzymes. With the increase of people’s awareness of furniture health care and environmental protection, the hazards of formaldehyde and benzene derivatives contained in chemical paints have been recognized by people. In home decoration, consumers are increasingly choosing environmentally friendly paints. Do not use raw lacquer as an important raw material. People pay more and more attention to raw lacquer. In the past 10 years, the price of raw lacquer has been ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCY02A40/22Y02P60/40
Inventor 何承忠赵月明李旦刘玉鲲段安安许玉兰祝建兵廖声熙
Owner SOUTHWEST FORESTRY UNIVERSITY
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