Method for producing sweet potato detoxified seedlings in water culture manner

A hydroponics and sweet potato technology, applied in the field of hydroponics to produce virus-free sweet potato seedlings

Inactive Publication Date: 2014-08-06
ACAD OF AGRI SCI ENSHI TUJIA MIAOAUTONOMOUS PREFECTURE
View PDF3 Cites 26 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, substrate cultivation cannot be used for rapid propagation and strong seedlings.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: a kind of hydroponic method of the present invention produces the method for sweet potato virus-free seedling, comprises the following steps: a. explant processing: select the high-yield high-quality high-quality or have special-purpose good sweet potato variety suitable for large-scale promotion in this area, Grow the selected potato pieces in the greenhouse. When the height of the seedlings is 20cm, select the 2cm-long buds at the top of the stem, cut off the leaves visible to the naked eye, rinse with tap water for 30min, soak in 70% alcohol for 25s, and then sterilize with 0.1% mercury solution for 30s. Disinfect with 5% sodium hypochlorite solution for 10 minutes, rinse with sterile water 3 times; b. shoot apical meristem culture: peel off 0.2-0.4mm shoot apex under a stereo microscope on an ultra-clean workbench, and inoculate it into MS plus hormone culture In the basal test tube, the temperature was 28 °C, the daily light was 16h, and the light inte...

Embodiment 2

[0019]Embodiment 2: a kind of hydroponic method of the present invention produces the method for sweet potato virus-free seedling, comprises the following steps: a. explant processing: select the high-yield high-quality high-quality or special-purpose good sweet potato varieties suitable for large-scale promotion in this area, Grow the selected potato pieces in the greenhouse. When the height of the seedlings is 20cm, select the 2cm-long buds at the top of the stem, cut off the leaves visible to the naked eye, rinse with tap water for 30min, soak in 70% alcohol for 25s, and then sterilize with 0.1% mercury solution for 30s. Disinfect with 5% sodium hypochlorite solution for 10 minutes, rinse with sterile water 3 times; b. shoot apical meristem culture: peel off 0.2-0.4mm shoot apex under a stereo microscope on an ultra-clean workbench, and inoculate it into MS plus hormone culture In the basal test tube, the temperature was 28 °C, the daily light was 16h, and the light intensit...

Embodiment 3

[0020]Embodiment 3: a kind of hydroponic method of the present invention produces the method for sweet potato virus-free seedling, comprises the following steps: a. explant processing: select the high-yield high-quality high-quality or special-purpose good sweet potato varieties suitable for large-scale promotion in this area, Grow the selected potato pieces in the greenhouse. When the height of the seedlings is 20cm, select the 2cm-long buds at the top of the stem, cut off the leaves visible to the naked eye, rinse with tap water for 30min, soak in 70% alcohol for 25s, and then sterilize with 0.1% mercury solution for 30s. Disinfect with 5% sodium hypochlorite solution for 10 minutes, rinse with sterile water 3 times; b. shoot apical meristem culture: peel off 0.2-0.4mm shoot apex under a stereo microscope on an ultra-clean workbench, and inoculate it into MS plus hormone culture In the basal test tube, the temperature was 28 °C, the daily light was 16h, and the light intensit...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for producing sweet potato detoxified seedlings in a water culture manner. Healthy and robust detoxified seedlings are obtained by adopting an improved MS minimal medium and a greenhouse hydroponic micropropagation method. The requirements of soilless cultivation ways based on vermiculite, pearlite matrix and the like are met, so that healthy and efficient sweet potato seed production is carried out. By adopting the method, the original characteristic that the tissue culture seedling is free from viruses, bacteria and insects is kept, the traditional breeding technique is innovated, the growth speed of the detoxified seedlings is greatly accelerated, the breeding cycle is shortened, and the production cost is greatly reduced. The water culture seedling is simple in operation procedure, and the hydroponic seedlings can be produced within 4-6 months in a large scale according to the production requirements. Therefore, the problem that the detoxified seedlings cannot be quickly at low cost in a large scale by the traditional seedling cultivation method is solved.

Description

technical field [0001] The invention relates to a method for producing virus-free sweet potato seedlings in a hydroponic manner. Background technique [0002] Sweet potato is a vegetatively propagated crop, which is mainly propagated through stems, tubers and seedlings, and is susceptible to virus infection during cultivation. Therefore, using shoot apical meristem to cultivate virus-free sweet potato seedlings is currently the only effective method in the world to prevent and control sweet potato virus diseases and improve the yield and quality of sweet potato. The original seed of virus-free sweet potato has the advantages of high variety purity, no virus, good storage and transportation performance, obvious yield increase effect, etc., and the potato pieces have uniform size, smooth surface, good commodity performance, high market price, and considerable economic benefits. The production of the original sweet potato seed has become the main measure for the rapid propagat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
CPCY02P60/21
Inventor 程群徐怡瞿勇李卫东沈艳芬向极钎郭光耀陈家吉陈巧玲朱云芬
Owner ACAD OF AGRI SCI ENSHI TUJIA MIAOAUTONOMOUS PREFECTURE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap