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Double-phase culture-medium for improving antiviral and nuclease-free grape-embryo crash breeding efficiency and its production

A technology of biphasic medium and seedless grapes, which is applied in the fields of botanical equipment and methods, gardening methods, gardening tools/equipment, etc., can solve the problems of lack of medium, hindering the rate of seedling formation, and the efficiency of embryo rescue needs to be improved.

Inactive Publication Date: 2007-01-17
NORTHWEST A & F UNIV
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AI Technical Summary

Problems solved by technology

However, in recent years, with the rapid development of seedless grape breeding technology and the continuous deepening of research, in the process of realizing the aggregation breeding of seedless genes and Chinese wild grape disease resistance genes, it has been shown that this technology still has many shortcomings, mainly The efficiency of embryo rescue needs to be improved, and it is difficult to meet the large demand for new seedless grape varieties in production
Experimental research shows that after using the original (ZL02139330.3) culture medium for ovule culture, the highest seedling rate does not exceed 20%, and most seedlings have weak growth potential, and it is difficult to survive when planted in a field, and the breeding efficiency is not ideal
At present, insufficient seedling rescue of embryos has become a bottleneck restricting the improvement of seedless grape breeding efficiency, and the lack of more suitable medium is the key link hindering the improvement of seedling rate. Seedless grape breeders all over the world are also working for confused by this question

Method used

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Embodiment Construction

[0056] Each component of the present invention and preferred concentration ratio are:

[0057] Calcium nitrate 0.017%

[0058] Potassium nitrate 0.066%

[0059] Potassium chloride 0.0075%

[0060] Ammonium nitrate 0.03%

[0061] Magnesium Sulfate 0.061%

[0062] Sodium dihydrogen phosphate 0.059%

[0063] Manganese sulfate 2.7×10 -4 %

[0064] Boric acid 5.0×10 -5 %

[0065] Zinc sulfate 1.9×10 -4 %

[0066] Cobalt chloride 1.4×10 -6 %

[0067] Copper sulfate 1.6×10 -6 %

[0068] Sodium molybdate 2.1×10 -6 %

[0069] Iron citrate 7.3×10 -4 %

[0070] Thiamine hydrochloride 2.5×10 -5 %

[0071] Pyridoxine hydrochloride 2.5×10 -5 %

[0072] Calcium D-pantothenate 2.5×10 -5 %

[0073] Niacin 2.5×10 -5 %

[0074] Glycine 1.0×10 -3 %

[0075] Inositol 5.0×10 -3 %

[0076] Hydrolyzed Casein 0.05%

[0077] L-cysteine ​​0.012%

[0078] Activated carbon 0.15%

[0079] Sucrose 6.0%

[0080] The rest is distilled water.

[0081] The preparation method of...

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Abstract

A two-phase culture medium for improving anucleate antiviral glucose embryo crash breeding efficiency and its production are disclosed. The procedure is carried out by preparing liquid-phase culture medium and solid-phase culture medium proportionally, using liquid to kill bacterium at high-pressure, pouring it into solid-phase culturing medium supernatant and preparing solid-phase culture medium. It has better anucleate antiviral glucose embryo development rate and breeding rate.

Description

technical field [0001] The invention relates to grape breeding technology, in particular to a dual-phase culture medium and a preparation method for improving the efficiency of disease-resistant seedless grape embryo rescue breeding, and belongs to the field of biotechnology. Background technique [0002] Grapes have become one of the main fruits that people love because of their rich nutrients and unique health functions. More than 80% of the table grapes in the United States are seedless varieties. At the same time, in the world, the widespread occurrence and prevalence of grape fungal diseases have also been concerned by breeding scientists. Therefore, cultivating high-quality, disease-resistant seedless grape varieties has become one of the common goals of grape breeding in various countries in the world. The breeding method of disease-resistant seedless grapes is an important link to provide high-quality varieties for production. Since the A...

Claims

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Application Information

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IPC IPC(8): C12N5/04A01H4/00
Inventor 王跃进田莉莉
Owner NORTHWEST A & F UNIV
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