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Method for simplifying saving of seedless grape embryo

A seedless grape, embryo rescue technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, to achieve the effect of accelerating the process of seedling emergence, saving manpower and material resources, and shortening the breeding cycle

Inactive Publication Date: 2011-03-02
HEBEI NORMAL UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, it has not been reported that seedlings can be successfully obtained by using only one medium for seedless grape embryo rescue from beginning to end.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] First choose potassium nitrate 950mg / L, ammonium nitrate 720mg / L, potassium dihydrogen phosphate 68mg / L, magnesium sulfate 185mg / L, calcium chloride 166mg / L, boric acid 3mg / L, manganese sulfate 22.3mg / L, zinc sulfate 10.0 mg / L, sodium molybdate 0.25mg / L, copper sulfate 0.08mg / L, niacin 0.5mg / L, thiamine hydrochloride 0.5mg / L, pyridoxine hydrochloride 0.5mg / L, glycine 2.0mg / L, VB5+ferric citrate+GA 3 3.0mg / L+IAA3.0mg / L mother solution and hormone, add 30g / L sucrose -1 , agar 3g / L -1 After the culture medium is dissolved and fixed to volume, the pH value is adjusted to 6, and then the sub-packed culture medium is put into an autoclave for sterilization. Sterilization conditions: pressure 1.05MPa, temperature 121°C, sterilization time 15min (up to After the pressure is required to start timing), the comprehensive culture medium is prepared, and then the seed-aborted seedless grape young fruits 72 days after full flowering are selected, rinsed with running water, disinfec...

Embodiment 2

[0022]The raw material components and concentration ratio of the comprehensive medium are: potassium nitrate 950mg / L, ammonium nitrate 720mg / L, potassium dihydrogen phosphate 68mg / L, magnesium sulfate 185mg / L, calcium chloride 166mg / L+ boric acid 3mg / L, Manganese sulfate 22.3mg / L, zinc sulfate 10.0mg / L, sodium molybdate 0.25mg / L, copper sulfate 0.08mg / L+nicotinic acid 0.5mg / L, thiamine hydrochloride 0.5mg / L, pyridoxine hydrochloride 0.5mg / L, glycine 2.0mg / L+VB50.25mg / L+ferric citrate 10mg / L+GA 3 0.1~3.0mg / L+IAA 0.1~3.0mg / L, sucrose 30g / L -1 -60g / L -1 , Agar 3g / L -1 -60g / L -1 . The steps of the seedless grape embryo rescue method are the same as the above-mentioned embodiment 1, so the description is omitted.

Embodiment 3

[0024] The raw material components and concentration ratio of the comprehensive medium are: potassium nitrate 950mg / L, ammonium nitrate 720mg / L, potassium dihydrogen phosphate 68mg / L, magnesium sulfate 185mg / L, calcium chloride 166mg / L+ boric acid 3mg / L, Manganese sulfate 22.3mg / L, zinc sulfate 10.0mg / L, sodium molybdate 0.25mg / L, copper sulfate 0.08mg / L+nicotinic acid 0.5mg / L, thiamine hydrochloride 0.5mg / L, pyridoxine hydrochloride 0.5mg / L, glycine 2.0mg / L+VB50.25mg / L+ferric citrate 10mg / L+GA 3 0.1~3.0mg / L+IBA 0.1~3.0mg / L, sucrose 30g / L -1 -60g / L -1 , Agar 3g / L -1 -60g / L -1 . The steps of the seedless grape embryo rescue method are the same as the above-mentioned embodiment 1, so the description is omitted.

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PUM

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Abstract

The invention relates to a method for simplifying saving of a seedless grape embryo, which is achieved by saving an in vitro embryo of the seedless grape embryo and comprises the following steps: preparing a synthetic medium, selecting and sterilizing young fruit, carrying out isolated culturing on the ovule, embryo stripping and embryo culture; selecting seed abortion seedless grape young fruit 72 days after flower blooming, washing the young fruit and sterilizing the young fruit at a clean bench respectively by corrosive sublimate and alcohol and stripping the ovule under aseptic conditions; then, inoculating the young fruit on the synthetic medium for carrying out dark culture, wherein the pH value of culture medium is 5.8-6.2 and culture temperature is 25-30 DEG C; carrying out embryo stripping treatment on the ovule after the ovule is cultured for 40-60 days; and continuing to inoculate the ovule to the synthetic medium and carrying out illumination culture on the ovule for 2000-4000LX. When the illumination culture is carried out on the ovule for 20-30 days, then embryo begins to germinate, a radicle gradually grows at the lower part of the embryo and two cotyledons gradually grown at the upper part of the embryo and finally the seedling is obtained. According to the method in the invention, a growth culture medium, a germination culture medium and a seedling formation culture medium are integrated into a whole and the saving of the seedless grape embryo is simplified and accelerated.

Description

technical field [0001] The invention relates to a method for simplifying seedless grape embryo rescue, which effectively simplifies the cultivation steps and accelerates the seedling formation process. Background technique [0002] Embryo rescue technology is to prepare a medium-the composition is similar to the nutrients supplied by the surrounding tissues in the embryo sac environment, and carry out in vitro culture before the abortion of zygotic embryos, so that the embryos that are about to be aborted continue to develop, and finally germinate into a complete embryo. plants. This technology realizes seedless plants that cannot be obtained by conventional cross-breeding. Since Haberlandt put forward the hypothesis of plant cell "totipotency" based on cell theory in 1902, plant embryo culture has developed rapidly. In 1904, Hanning successfully used the embryos of radish and horseradish for the first time to do systematic plant embryo culture on artificial medium. In 19...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 项殿芳闫淑芳王娜
Owner HEBEI NORMAL UNIVERSITY OF SCIENCE AND TECHNOLOGY
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