Centrifugal micro-fluidic device and method for immunoassay

A microfluidic device and immunoassay technology, which is applied in nanotechnology for sensing, measuring devices, biological testing, etc., can solve the problems of complex experimental steps and long test time, and achieve improved accuracy, sensitivity, and applicability Effect

Active Publication Date: 2013-02-13
PRECISIONBIOSENSOR INC
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] However, for immunoassays performed using conventional disk-type microfluidic devices in general clinical laboratories, the experimental steps are usually complicated and require a relatively long testing time

Method used

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  • Centrifugal micro-fluidic device and method for immunoassay
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  • Centrifugal micro-fluidic device and method for immunoassay

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Embodiment approach

[0061] According to an exemplary embodiment, the first reaction chamber 300 of the microfluidic device may contain a single label conjugate comprising one labeling substance to detect one analyte; however, the first reaction chamber 300 may also contain different labeling substances. Several labeled conjugates for the simultaneous detection of multiple analytes. For example, for the simultaneous detection of four different types of analytes, four types of label conjugates containing four different lanthanide(III) chelates (e.g., Eu, Sm, Dy, and Tb) as separate labeling species substances may be contained together in the first reaction chamber 300.

[0062] An advantage of the configuration described above is that a single microfluidic structure 1000 can be used to detect multiple analytes. Simultaneous detection of multiple analytes is also possible by using only one microfluidic device with several microfluidic structures built into it. This configuration is superior to con...

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Abstract

A centrifugal micro-fluidic device and an immunoassay method using the same are provided. The micro-fluidic device includes at least one micro-fluidic structure, the micro-fluidic structure including: a sample chamber receiving a fluid sample; a first reaction chamber which is connected with the sample chamber and contains at least one labeling conjugate; a second reaction chamber which is connected with the first reaction chamber and contains a capture binder; a buffer chamber which is connected with the second reaction chamber and contains an elution buffer; a detection chamber which is connected with the second reaction chamber and receives the at least one labeling conjugate; a plurality of channels through which the first reaction chamber, second reaction chamber, buffer chamber and detection chamber are interconnected; and at least one valve which is positioned in at least one of the plurality of channels, and opens and closes the channel.

Description

technical field [0001] Apparatus and methods consistent with the embodiments relate generally to centrifugal microfluidic devices and immunoassay methods using the same, and more particularly, to microfluidic devices using fluorescent nanoparticles to detect analytes in fluid samples and immunoassays using the same test methods. Background technique [0002] Microfluidic devices refer to devices that use small amounts of fluid to perform biological or chemical reactions. [0003] Typically, the microfluidic structure of a microfluidic device has at least one independent function and includes a chamber containing a fluid therein, a channel through which the fluid flows, and a valve for controlling the flow of the fluid. Microfluidic structures can be fabricated by combining these components in different ways. In particular, so-called "lab-on-a-chip" devices include microfluidic structures mounted on a substrate in a chip-like platform. With a lab-on-a-chip, experiments inv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/02G01N33/53G01N33/58
CPCG01N21/6428G01N33/54366G01N21/07B82Y15/00
Inventor 金仁煜
Owner PRECISIONBIOSENSOR INC
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