Production method for fluorescent microscopic slices of ovule of pollinated azalea

Abstract

The invention provides a production method for fluorescent microscopic slices of ovule of pollinated azalea. The production method comprises the following steps: sampling pistil of pollinated azalea; and fixing, softening, dyeing, stripping ovary walls and tabletting. In the steps of sampling and fixing, pollinated azalea flowers are picked, flower petals are stripped, only pistil parts comprising stigmas and ovaries are remained and are put into an FAA fixing solution to be fixed, and after 24 hours, the pistil parts are converted into a 70% ethanol solution to be stored; in the softening process, the fixed materials are put into a drying oven to be heated and softened; in the dyeing process, the softened materials are dyed with a 0.1% water-soluble aniline blue dyeing solution without light for one night; in the ovary wall stripping process, the dyed materials are subjected to ovary wall stripping treatment; and in the tabletting process, tabletting is performed on the stripped materials to obtain the slices. According to the method, the slices of ovule of pollinated azalea, which are clearly dyed and have complete tissue structure, can be obtained; and comprehensive fertilization information can be observed.

Description

technical field [0001] The invention relates to a method for making fluorescent microscopic slices, in particular to a method for making fluorescent microscopic slices of rhododendron pollinated ovules. Background technique [0002] Rhododendron occupies an important position in the world's gardens, and there is a saying in Europe that "no garden can't be completed without Rhododendron". Compared with developed countries in Europe and the United States, where there are many varieties of azaleas and are widely used, the varieties of azaleas cultivated and applied in landscaping in my country are relatively single, and mainly imported foreign varieties. my country is one of the main distribution centers of Rhododendron plants in the world. Among the wild species, there are many species with unique flower color, flower shape and fragrance. It is a breakthrough to cultivate new varieties of Rhododendron with independent intellectual property rights in my country. [0003] Our c...

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the disadvantages of the existing method of pollination style fluorescent micrographs that Rhododendron ovary is difficult to compress, ovules cannot be seen clearly or ovule structure is destroyed so that comprehensive fertilization information cannot be obtained, and its purpose is to provide Fluorescent microscopic tableting method for rhododendron pollination ovules with good tableting effect, clearly visible ovules, and clearly visible fertilization process

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