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Breeding method of gerbera jamesonii homozygote plant

A homozygous and plant technology, applied in the field of breeding of gerbera homozygous plants, can solve the problems of labor-intensive chromosome ploidy identification, low haploid doubling rate, poor rapid reproduction effect, etc. Omit complex procedures and overcome the complex effect of heterozygous state

Active Publication Date: 2013-05-22
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problem that the existing haploid plants obtained by tissue culture technology for ovule induction have low induction rate of ovule induction callus and young buds, and the genotype is not homozygous due to the presence of chimeras in the doubling process, and the chromosomes are not homozygous. Ploidy identification is labor-intensive, time-consuming, haploid doubling rate is low, homozygous plant identification and rapid propagation are difficult and other technical problems, providing a simple and fast method to obtain excellent homozygous plants of gerbera

Method used

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  • Breeding method of gerbera jamesonii homozygote plant
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  • Breeding method of gerbera jamesonii homozygote plant

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] A. Selection and pretreatment of explants:

[0061] ① Select the flower buds of F1 generation hybrids whose inflorescence diameter is 3 to 4 cm, and whose tongue-like flowers are 1 to 1.5 cm longer than the sepals;

[0062] ②Low temperature treatment, cut the pedicels of the flower buds, leave the pedicels 4-5cm long, insert them into a bottle with tap water, and put them into a refrigerator at 3°C ​​for low temperature treatment for 7 days;

[0063] ③ Wash the flower buds taken out of the refrigerator with washing powder water first, and then rinse them with clean water. In an ultra-clean environment, disinfect them with 0.1% mercury chloride solution for 15 minutes, and then put them into the following mixed solution for disinfection for 10 minutes. , and then rinsed with sterile water 5 times, each time for 1 min, to obtain sterilized flower buds; the mixed solution is: every 100ml of sodium hypochlorite solution with a mass concentration of 2% was added dropwise 2 d...

Embodiment 2

[0088] Embodiment 2 is the same as Embodiment 1 except for the following operations, and will not be repeated here.

[0089] A. Selection and pretreatment of explants

[0090] ②The temperature of the refrigerator in the low-temperature treatment is 6°C, and the low-temperature treatment is 3 days;

[0091] B, the culture medium of callus induction and sprout differentiation are:

[0092] MS Basic Medium

[0093]

[0094] First culture at a temperature of 27°C in the dark for 15 days, and then switch to a light intensity of 2000lx, a light time of 10h / d, and a temperature of 27°C, and simultaneously carry out callus induction and shoot differentiation culture to explants After germination and differentiation of young shoots larger than 1 cm, within 60 days, the rate of ovule induction into buds reached 28% (28 ovules out of 100 ovules were induced to form buds).

[0095]C. Subculture

[0096] The light intensity of subculture is 2000lx, the temperature is 27°C, the light...

Embodiment 3

[0108] Embodiment 3 is the same as Embodiment 1 except for the following operations, and will not be repeated here.

[0109] A. Selection and pretreatment of explants:

[0110] ②The temperature of the refrigerator in the low-temperature treatment is 4°C, and the low-temperature treatment is performed for 5 days.

[0111] B, the culture medium of callus induction and sprout differentiation are:

[0112] MS Basic Medium

[0113]

[0114] First culture at a temperature of 25°C and in the dark for 15 days, and then switch to the condition of light intensity of 1800lx, light time of 11h / d, and temperature of 25°C, simultaneously carry out callus induction and shoot differentiation culture to explants After germination and differentiation of young shoots larger than 1 cm, within 60 days, the rate of ovule induction into buds reached 36% (36 ovules were induced to buds out of 100 ovules).

[0115] C. Subculture:

[0116] The light intensity of the subculture was 1800lx, the te...

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Abstract

The invention provides a breeding method of a gerbera jamesonii homozygote plant, belonging to the field of biotechnology. The breeding method comprises the steps of: through flower bud selection, low-temperature treatment and disinfection, picking off ovules and introducing into a same culture medium for synchronously carrying out callus induction, bud differentiation and subculture, carrying out chromosome reduplication on a primarily selected haplobiont, primarily selecting a reduplicated strain for expanding propagation and rooting culture, hybridizing in field strains, and selecting again to obtain an excellent homozygote plant. According to the invention, the callus induction, bud differentiation and subculture of ovules are carried out by using one culture medium, and thus a tissue culture flow is simplified; and by adopting a method of combining indoor indirect evaluation and field direct evaluation, the step of evaluating the ploidy of chromosome is omitted, the difficulty that a genotype is not homozygous due to a chimera existing in an obtaining and reduplication process of the haplobiont is avoided, the gerbera jamesonii homozygote plant can be simply and rapidly bred and propagated, and a selfing life with excellent and stable characters is provided for the breeding of new variety.

Description

technical field [0001] The invention relates to a method for breeding homozygous gerbera plants, belonging to the field of biotechnology. Background technique [0002] Gerbera jamesonil Bolus (Gerbera jamesonil Bolus), also known as gerbera, is a perennial herbaceous flower of the genus Gerbera in the family Asteraceae. It is one of the five largest fresh cut flowers in the world. It can be used for cut flower production or potted ornamental, and is widely distributed all over the world. Because of its gorgeous colors, huge flowers, beautiful flowers and its unique cultural connotation, it is favored by consumers. [0003] At present, there is an urgent need for high-quality, high-yielding new gerbera varieties that are resistant to low temperature, low light, and pests and diseases. The heterosis between inbred lines is greater than that between varieties, and gerbera is a typical cross-pollination crop. Due to long-term asexual reproduction, it retains high heterozygosity...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
Inventor 单芹丽杨春梅李绅崇王继华吴丽芳屈云慧汪国鲜曹桦张婷许凤李金泽阮继伟
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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