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Breeding method of chrysanthemum haploid

A chrysanthemum and sports technology, applied in the field of breeding, can solve the problem of difficulty in obtaining homozygotes, and achieve the effect of huge potential commercial value

Inactive Publication Date: 2007-08-08
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Research purposes in chrysanthemums: chrysanthemums are self-sterile plants, so

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Test material: Chrysanthemum 'Donglin Ruixue' (Beijing Linda Forestry Technology Co., Ltd.)

[0017] After picking buds of different sizes every morning, the morphological characteristics such as bud length and bud diameter of each bud were measured respectively. Finally, use tweezers to peel off the sepals and petals, take out the florets, put them on a glass slide, peel off the anthers, observe the color of the anthers, stain with carbo magenta, and examine under a microscope to determine the relationship between the pollen development period and the diameter of the flower buds and the appearance of the anthers sex. Since there are great differences in the external morphology of flower buds among different varieties, the development period of microspores can be judged by observing the color of florets. It was found that the floret is yellow, and the corresponding microspore development stage is mature pollen grains, and the floret is white, and the corresponding micr...

Embodiment 2

[0067] Take the flower buds at the marginal stage of mononuclei, soak them in 70% alcohol for 30 seconds, then disinfect them with 20% sodium hypochlorite solution for 10 minutes, and rinse them with sterile water. Take out the florets, peel off the anthers under a dissecting microscope, remove the filaments, and try to prevent the anthers from being damaged. Inoculate the anthers in a liquid medium without agar at room temperature, place them at a low temperature of 4°C for 48 hours, and then place them on a shaker. Cultivate to make the pollen scatter naturally in the culture medium during the shaking process, cultivate for 15 days, pick out the anther wall, and continue to cultivate. Culture conditions: 26±0.5°C during the day, 23±0.5°C at night, 14h / d light intensity 1500lx. After culturing for 40 days, the statistical healing rate was 21.4%.

[0068] The formula of the liquid medium is MS+BA 2.0mg / L+2,4-D 1.0mg / L, which does not contain agar.

[0069] The cultured callu...

Embodiment 3

[0070] Ploidy Identification of Example 3 Pollen Cultured Plants

[0071] Take the root tip of the pollen plant, press it with conventional chromosome production technology, pretreat with 8-hydroxyquinone or p-dichlorobenzene saturated solution for 3.5 hours, fix with Carnot’s fixative for 2-24 hours, wash with 50% ethanol, and rinse with distilled water. Dissociate with 1M hydrochloric acid at 600C for 7-8 minutes, wash away the hydrochloric acid, stain with carbo fuchsin for 3-5 minutes, make slides, examine under a microscope, and identify haploids by counting chromosomes.

[0072] The differentiated individual plants were examined under the microscope by the above method, and the haploid plants were detected after chromosome counting, and then naturally doubled, or artificially treated with colchicine hormones on the shoot apex growth point, to become pure and diploid.

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Abstract

The invention relates to a method for planting the monoploid of chrysanthemum. The inventive method finds the external shape mark of chrysanthemum pollen at single corn period, and uses said pollen to induce the callus to obtain the monoploid plant. The invention uses liquid medium to cultivate the pollen and pollen wall, to improve the differentiation ablity of callus. For the chrysanthemum with self-cross and sterility, the inventive pollen cultivation can obtain the monoploid and pure diploid, to be used in cross generation to obtain the better F1 generation quickly.

Description

technical field [0001] The invention relates to a breeding method, in particular to a haploid breeding method of chrysanthemums. Background technique [0002] With the development of plant tissue culture technology, the "totipotency" of plant cells has been widely confirmed. Therefore, under in vitro conditions, anthers are cultivated in vitro, and the developmental pathway of microspores is artificially changed to make their gametophytes develop. The pathway is terminated and turned to sporophyte development. Through the pathway of organogenesis or embryogenesis, a complete haploid plant is formed, which provides an effective means for artificial mass production of haploids. The haploid plants regenerated through this approach can be doubled spontaneously or artificially induced to obtain homozygous diploid plants, thus providing useful materials for further breeding and genetic research. The use of anther pollen to obtain haploids for breeding is a major innovation in pla...

Claims

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Application Information

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IPC IPC(8): A01H1/08A01H4/00A01H5/00C12N5/04
Inventor 高亦珂王伟光张启翔
Owner BEIJING FORESTRY UNIVERSITY
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