Breeding method of chrysanthemum haploid

Abstract

The invention relates to a method for planting the monoploid of chrysanthemum. The inventive method finds the external shape mark of chrysanthemum pollen at single corn period, and uses said pollen to induce the callus to obtain the monoploid plant. The invention uses liquid medium to cultivate the pollen and pollen wall, to improve the differentiation ablity of callus. For the chrysanthemum with self-cross and sterility, the inventive pollen cultivation can obtain the monoploid and pure diploid, to be used in cross generation to obtain the better F1 generation quickly.

Description

technical field

[0001] The invention relates to a breeding method, in particular to a haploid breeding method of chrysanthemums. Background technique

[0002] With the development of plant tissue culture technology, the "totipotency" of plant cells has been widely confirmed. Therefore, under in vitro conditions, anthers are cultivated in vitro, and the developmental pathway of microspores is artificially changed to make their gametophytes develop. The pathway is terminated and turned to sporophyte development. Through the pathway of organogenesis or embryogenesis, a complete haploid plant is formed, which provides an effective means for artificial mass production of haploids. The haploid plants regenerated through this approach can be doubled spontaneously or artificially induced to obtain homozygous diploid plants, thus providing useful materials for further breeding and genetic research. The use of anther pollen to obtain haploids for breeding is a major innovation in pla...