Method for extracting pollen amino acid liquid from rose pollen
A technology of rose pollen and amino acid, applied in the direction of separation methods, chemical instruments and methods, solvent extraction, etc., can solve the problems of chemical reagent residue, waste of resources, high noise, etc., to eliminate heavy metal pollution, eliminate noise pollution, and reduce production costs Effect
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Embodiment 1
[0019] Example 1, the method for extracting pollen amino acid liquid from rose pollen is carried out as follows: first step, take an appropriate amount of rose pollen, place the rose pollen at a temperature of -25°C to -15°C for 48h to 72h, and then Add 10 to 20 times the volume of rose pollen in boiling water and mix evenly to obtain a pollen liquid. The pollen liquid evaporates to 1 / 3 to 1 / 5 of the volume of the pollen liquid to obtain a pollen concentrate; the second step is to add 3 times to the pollen concentrate The aqueous hydrogen peroxide solution to 10 times the quality of rose pollen, the mass percentage concentration of the aqueous hydrogen peroxide solution is 3%, and the reaction is stirred for 12h to 24h after adding, and the first suspension is obtained after the reaction after stirring; the third step, in the first suspension Add 10 to 20 times the mass of rose pollen water to the turbid liquid, shake and wash and let it stand for 8 to 15 hours, then centrifu...
Embodiment 2
[0020] Example 2, the method for extracting pollen amino acid liquid from rose pollen is carried out as follows: first step, take an appropriate amount of rose pollen, place the rose pollen at a temperature of -25°C or -15°C for 48h or 72h, and then Add 10 times or 20 times the mass of rose pollen in boiling water and mix evenly to obtain a pollen liquid. After the pollen liquid evaporates to 1 / 3 or 1 / 5 of the volume of the pollen liquid, a concentrated pollen liquid is obtained; in the second step, add 3 times Or an aqueous hydrogen peroxide solution with 10 times the quality of rose pollen, the mass percentage concentration of the aqueous hydrogen peroxide solution is 3%, and after adding, stir and react for 12h or 24h, and obtain the first suspension after stirring and react; the third step, in the first suspension Add 10 times or 20 times the quality of rose pollen water to the turbid liquid, shake and wash it and let it stand for 8 hours or 15 hours. Water with 20 times...
Embodiment 3
[0021] Example 3, the method for extracting pollen amino acid liquid from rose pollen is carried out as follows: first step, take 1.00 g of rose pollen, place the rose pollen at -20°C for 48 hours, and then add 20 g of boiling water Mix evenly to obtain pollen liquid, pollen liquid is evaporated to 1 / 3 of the volume of pollen liquid to obtain pollen concentrated liquid; in the second step, add 10ml of hydrogen peroxide aqueous solution with a percentage concentration of 3% to the pollen concentrated liquid, stir and react for 24 hours after adding , after stirring and reacting, the first suspension was obtained; the third step was to add 10ml of water to the first suspension, shake and wash and let it stand for 8 hours, then centrifuge after standing, and obtain a precipitate after centrifugation; the fourth step , add 10ml of water to the first precipitation, shake and wash and then centrifuge to obtain the second precipitation; the fifth step, repeat the fourth step twice t...
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