Method for extracting pollen amino acid liquid from rose pollen

A technology of rose pollen and amino acid, applied in the direction of separation methods, chemical instruments and methods, solvent extraction, etc., can solve the problems of chemical reagent residue, waste of resources, high noise, etc., to eliminate heavy metal pollution, eliminate noise pollution, and reduce production costs Effect

Active Publication Date: 2016-01-06
XINJIANG YUTIAN QUEME BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a method for extracting pollen amino acid liquid from rose pollen, which overcomes the above-mentioned deficiencies in the prior art, and can effectively solve the problem of broken pollen products in existing pollen products. Metal scraps are easily brought into the

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  • Method for extracting pollen amino acid liquid from rose pollen

Examples

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Effect test

Embodiment 1

[0019] Example 1, the method for extracting pollen amino acid liquid from rose pollen is carried out as follows: first step, take an appropriate amount of rose pollen, place the rose pollen at a temperature of -25°C to -15°C for 48h to 72h, and then Add 10 to 20 times the volume of rose pollen in boiling water and mix evenly to obtain a pollen liquid. The pollen liquid evaporates to 1 / 3 to 1 / 5 of the volume of the pollen liquid to obtain a pollen concentrate; the second step is to add 3 times to the pollen concentrate The aqueous hydrogen peroxide solution to 10 times the quality of rose pollen, the mass percentage concentration of the aqueous hydrogen peroxide solution is 3%, and the reaction is stirred for 12h to 24h after adding, and the first suspension is obtained after the reaction after stirring; the third step, in the first suspension Add 10 to 20 times the mass of rose pollen water to the turbid liquid, shake and wash and let it stand for 8 to 15 hours, then centrifu...

Embodiment 2

[0020] Example 2, the method for extracting pollen amino acid liquid from rose pollen is carried out as follows: first step, take an appropriate amount of rose pollen, place the rose pollen at a temperature of -25°C or -15°C for 48h or 72h, and then Add 10 times or 20 times the mass of rose pollen in boiling water and mix evenly to obtain a pollen liquid. After the pollen liquid evaporates to 1 / 3 or 1 / 5 of the volume of the pollen liquid, a concentrated pollen liquid is obtained; in the second step, add 3 times Or an aqueous hydrogen peroxide solution with 10 times the quality of rose pollen, the mass percentage concentration of the aqueous hydrogen peroxide solution is 3%, and after adding, stir and react for 12h or 24h, and obtain the first suspension after stirring and react; the third step, in the first suspension Add 10 times or 20 times the quality of rose pollen water to the turbid liquid, shake and wash it and let it stand for 8 hours or 15 hours. Water with 20 times...

Embodiment 3

[0021] Example 3, the method for extracting pollen amino acid liquid from rose pollen is carried out as follows: first step, take 1.00 g of rose pollen, place the rose pollen at -20°C for 48 hours, and then add 20 g of boiling water Mix evenly to obtain pollen liquid, pollen liquid is evaporated to 1 / 3 of the volume of pollen liquid to obtain pollen concentrated liquid; in the second step, add 10ml of hydrogen peroxide aqueous solution with a percentage concentration of 3% to the pollen concentrated liquid, stir and react for 24 hours after adding , after stirring and reacting, the first suspension was obtained; the third step was to add 10ml of water to the first suspension, shake and wash and let it stand for 8 hours, then centrifuge after standing, and obtain a precipitate after centrifugation; the fourth step , add 10ml of water to the first precipitation, shake and wash and then centrifuge to obtain the second precipitation; the fifth step, repeat the fourth step twice t...

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Abstract

The invention relates to the technical field of plant extraction and provides a method for extracting a pollen amino acid liquid from rose pollen. The method comprises steps as follows: step one, the rose pollen is processed, and then boiling water is added for evaporation; step two, a hydrogen dioxide solution is added; step three, water is added, washing is performed, then the mixed solution is left to stand, primary precipitate is obtained through centrifugation, and after the primary precipitate is washed for 3-4 times, final precipitate is obtained; step four, a phosphate buffer solution, cellulase and lysozyme are added to the final precipitate, the mixture is subjected to enzymolysis and then is boiled and centrifuged, and a supernatant is taken as the pollen amino acid liquid. The extraction process does not involve metal grinding, avoids noise and strong corrosive substances and eliminates heavy metal pollution and noise pollution of products; the pollen amino acid liquid contains pollen active substances, is free of chemical agent residues, removes sensitization effect of residual pollen wall and is suitable for being taken as a raw material of health care products and cosmetics; meanwhile, rose liquid waste obtained after essential oil extraction is recycled, so that the rose pollen resource is effectively utilized, and the production cost is greatly reduced.

Description

technical field [0001] The invention relates to the technical field of plant extraction, and relates to a method for extracting pollen amino acid liquid from rose pollen. Background technique [0002] Pollen is the male gametophyte of sexual reproduction in plants. It is usually composed of vegetative cells and reproductive cells wrapped in the pollen wall. It contains a variety of amino acids, vitamins, esters, flavonoids, minerals, nucleic acids and other nutrients. All the substances required for tube germination except water are possessed by the pollen itself, and its nutrition is very balanced and concentrated. It has been documented that pollen has a strengthening effect on liver superoxide dismutase, which can effectively resist free radicals and delay aging; at the same time, the active ingredients in pollen can stimulate the body's immune system, enhance human immunity, and protect the liver; in addition, pollen It can regulate endocrine, and can have a certain hea...

Claims

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Application Information

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IPC IPC(8): B01D11/00B01D11/02
Inventor 陈秋鹏欧秀玲黄荣萍李勇唐丽薇
Owner XINJIANG YUTIAN QUEME BIOTECH CO LTD
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