Application of male-sterility gene OsGEN and method for restoring fertility

A male sterility gene and male sterility technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve problems such as the limitations of restorer lines and maintainer lines, and the complex performance of three-line hybrid rice seeds.
CN105950651AInactive Publication Date: 2016-09-21SHANGHAI JIAO TONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI JIAO TONG UNIV
Publication Date
2016-09-21
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention specifically relates to application of a male-sterility gene OsGEN and a method for restoring fertility, belonging to the technical field of rice breeding. The male-sterility gene OsGEN has an amino acid sequence as shown in SEQ ID No. 1. The application of the male-sterility gene OsGEN is that the OsGEN gene is knocked out, changed or inhibited by using a conventional method or based on a CRISPR / Cas9 system so as to allow the expression level of the OsGEN gene in a conventional rice variety to be decreased, and thus, a male-sterility strain of rice is obtained. The invention also relates to the method for restoring male sterility of rice caused by deletion of the OsGEN gene. According to the method, the OsGEN gene is amplified through primers, a mutant plant is transformed by using a genetic transformation means, so the mutant is restored to the phenotype of a wild type. The rice mutant obtained in the invention is free of abnormity in the phase of vegetative growth; however, a homozygote plant is completely infertile. When the mutant is applied to cross breeding, the operation of emasculation of a female parent can be omitted; production efficiency is substantially improved; labor cost is reduced; and the mutant has important application value in agricultural production.
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Description

technical field

[0001] The invention relates to a method for creating rice strains in the technical field of bioengineering, in particular to an application of the male sterility gene OsGEN and a method for restoring fertility. Background technique

[0002] Rice is one of the main food crops in the world, and it is the most important food crop in my country. More than 60% of the people in China take rice as the staple food, and China is the largest rice producer and consumer in the world. Hybrid rice can greatly increase the yield of rice and is an important method to solve the food problem. The hybrid rice currently used in production belongs to the three-line hybrid rice, which increases the yield by about 20% compared with conventional rice. However, the expression of seed dominance in three-line hybrid rice is complicated and limited by restorer lines and maintainer lines. Therefore, scientists have been screening and breeding new sterile lines to lay the foundation f...

Claims

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