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Artificial emasculation and hybrid control pollination method of robinia pseudoacacia L.

A technology for artificial emasculation and control of pollination, applied in the fields of botanical equipment and methods, plant genetic improvement, application, etc., can solve the problems of artificial pollination, low seed setting rate, natural seed setting rate, limited success rate of locust breeding, flower damage, etc. The effect of improving pollination seed setting rate, natural seed setting rate and success rate

Inactive Publication Date: 2016-03-23
SHANDONG FOREST SCI RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, when the locust flower is open, the anthers and stigmas cannot be seen from the outside, and the stamens and pistils can only be exposed by pulling the petals with the help of tools, which brings great difficulty to artificial pollination in hybrid breeding.
Artificial pollination not only removes the stamens, but also removes the petals to expose the stigma, which brings damage to the flowers, affects their normal physiological and biochemical processes, and makes the seed setting rate of artificial pollination lower than the natural seed setting rate.
The natural seed setting rate of Robinia pseudoacacia is low, an average of 30%, which limits the success rate of artificial cross breeding of Robinia pseudoacacia
The result of our experiment is that, using conventional methods to artificially pollinate Robinia pseudoacacia, the seed setting rate is 1% to 5%. In addition, in the literature "Flowering Pollination and Mating Mode of Robinia pseudoacacia" Journal of Northeast Forestry University, the average fruit setting rate of artificial emasculation hybridization is only 3.56%. %; the so far reported seed setting rate of artificial pollination of Robinia pseudoacacia is also within this range

Method used

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  • Artificial emasculation and hybrid control pollination method of robinia pseudoacacia L.
  • Artificial emasculation and hybrid control pollination method of robinia pseudoacacia L.
  • Artificial emasculation and hybrid control pollination method of robinia pseudoacacia L.

Examples

Experimental program
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Effect test

Embodiment 1

[0043] The invention provides a method for artificial detasseling and hybridization control pollination of Robinia pseudoacacia, comprising the following steps:

[0044] (1) Make a pollinator: Cut the rubber 4 into a cylinder with a length of 2 cm and a diameter of 1 cm, sharpen one end for sticking pollen, flatten the other end, insert a thin iron wire 3 in the middle, and use it with the glass bottle Rubber bottle stopper 2 connections, the length of the thin iron wire is about 10cm, see figure 1 and figure 2 ; Among them, the height of the glass bottle is 5cm, the diameter is 2cm, and the cylindrical rubber stopper.

[0045] (2) Select the hybrid male parent and female parent plant, and select the robust tree of 6-20 years old in the middle and young forest of Robinia pseudoacacia fine variety that has entered the reproductive growth stage as the hybrid parent plant. Prune the mother tree properly in early spring, thin out the inner hall branches, and cut off the dead b...

Embodiment 2

[0051] The conventional artificial pollination method of Robinia pseudoacacia is to select inflorescences suitable for pollination of the mother plant, emasculate the flowers, cut off the petals in the upper and middle parts of the corolla with scissors, expose the stamens and pistils, then cut off the stamens, or remove the anthers by hand, and dip them in with a brush. A little pollen smears the stigma to pollinate the stigma. After the pollination of the entire inflorescence is complete, bag and hang tags.

experiment example 1

[0053] In 2014, the method of Example 1 was used to form a hybrid combination of different clones of Robinia pseudoacacia, and artificially controlled pollination was implemented. The result was that the seed setting rate of the Luci 57×Xingci 15 hybrid combination was 21.28%, and the seed setting rate of Xingci 1×Luci 68 was 16.82%, and that of Luci 152×Jinci 4 was 13.05%. See Table 1.

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Abstract

The invention discloses an artificial emasculation and hybrid control pollination method of robinia pseudoacacia L.. The method comprises the following steps of pollenator making, selection of hybrid male parent and female parent plants, pollen collecting, artificial pollination, pollinated female plant management and hybrid ripe pod harvesting. According to the artificial emasculation and hybrid control pollination method of the robinia pseudoacacia L., the technical steps of emasculation and pollination for the robinia pseudoacacia L. are described in detail, petals and pistil are not damaged, the artificial pollination ripening rate of the robinia pseudoacacia L. can be significantly increased, and the artificial hybridization breeding success rate of the robinia pseudoacacia L. is increased.

Description

Technical field [0001] The invention belongs to the technical field of plant breeding, and specifically relates to a method for artificial emasculation and hybrid controlled pollination of black locust. Background technique [0002] Robiniapseudoacacia L. is a large broad-leaved tree of the Fabaceae subfamily Papilioideae. It is native to the United States and was introduced to Europe in the early 17th century (1601). By the 18th century, it spread throughout Europe. The Germans first introduced black locust seeds to Qingdao, China, at the end of the 19th century. From the 1940s to the 1960s, several batches of black locust seeds from Japan, the United States, and North Korea were planted in Liaodong, Tianshui, Gansu, Changsha, Hunan, and North China. In the late 1980s, 24 provinces, municipalities, and autonomous regions in China planted black locust trees. Only 6 provinces, including Hebei, Henan, Shandong, Shanxi, and Liaoning, had 4 billion trees, making it the most popu...

Claims

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Application Information

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IPC IPC(8): A01H1/02
CPCA01H1/02A01H1/027
Inventor 荀守华孙百友乔玉玲张自和张元帅董元夫毛秀红姜丽娜
Owner SHANDONG FOREST SCI RES INST
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