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Culture medium for germination of tetraploid hemarthria compressa pollen

A technology for the germination of verbena and pollen, which is applied to plant cells, etc., can solve the problems of lack of medium and achieve the effects of increasing germination rate, pollination rate and natural seed setting rate, and promoting absorption and metabolism

Inactive Publication Date: 2014-08-27
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is of great significance to study it from the perspective of pollen germination to overcome its "flowers but not fruit". At present, there are few studies on the medium for pollen germination of Verbena flat tachycarpus, and there is no corresponding suitable for tetraploid flat panicle. Media for Verbena Pollen Germination

Method used

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  • Culture medium for germination of tetraploid hemarthria compressa pollen
  • Culture medium for germination of tetraploid hemarthria compressa pollen
  • Culture medium for germination of tetraploid hemarthria compressa pollen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Prepare five groups of culture media, the concentration of boric acid of these five groups of culture media is 30mg / L, vitamin B 1 The concentration is 10mg / L, the sucrose concentration is 135g / L, 155g / L, 175g / L, 195g / L, 215g / L respectively, and the above five groups of culture media are used to germinate the pollen of tetraploid verbena tachycarpa , and then the pollen germination rate was measured, and the results were as follows figure 1 , figure 2 shown, where figure 1 is the measurement result graph of pollen germination rate, figure 2 is the measurement result graph of pollen tube length;

[0023] Depend on figure 1 , 2 It can be seen that with the increase of sucrose concentration, the pollen germination rate of Verbena flat tachycarpa gradually increased, and the growth length of pollen tubes also increased. The tube length is also the longest, with an average length of 205.58 μm. When the sucrose concentration is higher than 195 g / L, the pollen germinat...

Embodiment 2

[0025] Prepare five groups of culture medium, the sucrose concentration of these five groups of culture medium is 175g / L, vitamin B 1 The concentration is 10mg / L, the concentration of boric acid is 10mg / L, 20mg / L, 30mg / L, 40mg / L, 50mg / L respectively, and the above five groups of culture media are used to germinate the pollen of tetraploid verbena , and then the pollen germination rate was measured, and the results were as follows image 3 , Figure 4 shown, where image 3 is the measurement result graph of pollen germination rate, Figure 4 is the measurement result graph of pollen tube length;

[0026] Depend on image 3 , 4 It can be seen that a certain concentration of boric acid can promote the pollen germination rate and pollen tube growth length. When the boric acid concentration is higher than 40mg / L, the pollen germination rate and pollen tube growth length are significantly inhibited. When the boric acid concentration is 40mg / L When the concentration of boric ac...

Embodiment 3

[0028] Prepare five groups of culture media, the sucrose concentration of these five groups of culture media is 175g / L, the boric acid concentration is 30mg / L, vitamin B 1 Concentrations were 0mg / L, 5mg / L, 10mg / L, 15mg / L, 20mg / L, and the above five groups of culture media were used to germinate the pollen of tetraploid Verbena flat tachycarpa, and then the pollen germination rate was determined. Measurement results such as Figure 5 , Figure 6 shown, where Figure 5 is the measurement result graph of pollen germination rate, Figure 6 is the measurement result graph of pollen tube length;

[0029] Depend on Figure 5 , 6 It can be seen that, along with vitamin B 1 With the increase of concentration, the pollen germination rate and pollen tube growth length have some changes, but the changes are not drastic. When vitamin B 1 When the concentration of vitamin B1 was 10mg / L, the highest pollen germination rate was 56.57%, and the longest pollen tube growth length was 183....

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Abstract

The invention discloses a culture medium for germination of tetraploid hemarthria compressa pollen, and the culture medium is capable of improving the pollen germination rate. The culture medium is an aqueous solution formed by a solvent and a solute, wherein water is taken as the solvent; the solute comprises sucrose, boric acid and vitamin B1; the concentration of the sucrose in the aqueous solution is between 135g / L and 215g / L; the concentration of the boric acid in the aqueous solution is between 10mg / L and 50mg / L; the concentration of the vitamin B1 in the aqueous solution is between 0mg / L and 20mg / L. When the culture medium is used for germinating the tetraploid hemarthria compressa pollen, the pollen germination rate is greatly improved, so that the pollination rate and natural maturing rate of the pollen are improved. Thus, the culture medium disclosed by the invention is suitable for being popularized in the field of plant culture.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and in particular relates to a culture medium for the germination of tetraploid verbena platyscens pollen. Background technique [0002] Verbena compressa (Hemarthria compressa), as a high-quality grass forage, belongs to the rhizome plant of the genus Verbena, because it is a herbaceous C 4 Plants are mostly distributed in areas with high accumulated temperature, such as tropical and subtropical regions, and only a few are distributed in temperate humid areas with slightly lower accumulated temperature. It has many excellent characteristics, but because of its low natural seed setting rate, it brings a lot of trouble to breeding researchers. The quality and quantity of pollen are the important internal conditions to determine the pollination and fruit setting of Verbena flat tachycarpa. It is of great significance to study it from the perspective of pollen germination to overcome its "flowers ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
Inventor 杨春华刘刚澹台国银李达旭刘琳孙飞达刘伟余克非杨勇
Owner SICHUAN AGRI UNIV
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