Seedling treatment composition and application thereof in improving stress resistance of solanaceous vegetables and promoting growth of solanaceous vegetables

Abstract

The invention belongs to the technical field of vegetable planting, and particularly relates to a seedling treatment composition and application thereof in improving stress resistance of solanaceous vegetables and promoting growth of the solanaceous vegetables. According to the method, solanaceous vegetable seeds are soaked in the seed treatment liquid obtained by dissolving the seedling treatment composition in water, the soaked seeds are subjected to dark culture, and the seedling treatment liquid is sprayed in the solanaceous vegetable seedling stage, so that the low temperature resistance of solanaceous vegetables can be effectively improved; the vigor of solanaceous vegetable seeds and the quality of seedlings under the low-temperature condition are improved. The test result shows that the seedling emergence potential, the seedling emergence rate and the seedling emergence index of the solanaceous vegetable seeds under the low-temperature condition can be improved and the average seedling emergence time can be shortened by using the seedling treatment liquid and culturing mode, and meanwhile, the chlorophyll content, the plant height, the root length and the fresh weight of the solanaceous vegetable seedlings under the low-temperature condition can be improved.

Description

Technical Field

[0001] The invention belongs to the technical field of vegetable planting, and particularly relates to a seedling treatment composition and application thereof in improving stress resistance of solanaceous vegetables and promoting growth of solanaceous vegetables. Background Art

[0002] During the process of raising seedlings, early planting or delayed planting, Solanaceae vegetables often encounter low temperature chilling and freezing damage, which has a great impact on seed germination and seedling growth, and may cause plant death in severe cases. In order to deal with chilling damage to Solanaceae vegetables, the existing technology mostly prevents chilling damage to Solanaceae vegetables by means of heat preservation, temperature increase, humidity reduction and leaf maintenance. At the same time, after Solanaceae vegetables encounter chilling damage, remedial measures such as temperature and humidity control and fertilizer and water management are used to reduce the losses caused by chilling damage to Solanaceae vegetables.

[0003] Existing studies have shown that low-temperature treatment or temperature-changing treatment of Solanaceae vegetable seeds can improve the cold resistance of seeds. For example, the seeds that are about to germinate after swelling are placed at a temperature of about 0°C and frozen for 1 to 2 days before sowing, which can promote germination and enhance the cold resistance of seedlings. The Solanaceae seeds that are about to germinate are placed at a lower temperature of 1 to 5°C for 12 to 18 hours every day, and then transferred to a higher temperature of 18 to 22°C for 6 to 12 hours. Repeating this for several days can significantly improve the cold resistance of vegetable seedlings and accelerate their growth and development. Although this method can improve the cold resistance of Solanaceae seeds to a certain extent and solve the problem of low germination rate under low temperature, it cannot guarantee the quality and normal growth of Solanaceae vegetable seedlings after encountering low temperatures. Summary of the invention

[0004] The purpose of the present invention is to provide a seedling treatment composition and its application in improving the stress resistance of Solanaceous vegetables and promoting the growth of Solanaceous vegetables. After using the seedling treatment composition to treat Solanaceous vegetable seeds and seedlings, the stress resistance of Solanaceous vegetables and the vitality of seeds and seedling quality under low temperature conditions can be effectively improved.

[0005] The invention provides a seedling treatment composition, comprising chitosan oligosaccharide, D-cellobiose and xylo-oligosaccharide, wherein the mass ratio of the chitosan oligosaccharide, D-cellobiose and xylo-oligosaccharide is (1-2): (1-2): (1-2).

[0006] Preferably, the relative molecular mass of the chitosan oligosaccharide is less than 3000, and the degree of polymerization is 2-10; the relative molecular mass of the xylooligosaccharide is 300-1051.

[0007] The present invention also provides the use of the seedling treatment composition described in the above technical solution in improving the stress resistance of Solanaceae vegetables and / or promoting the growth of Solanaceae vegetables.

[0008] Preferably, the improving stress resistance of Solanaceous vegetables includes improving seed vigor under low temperature conditions and / or improving the low temperature resistance of Solanaceous vegetable seedlings.

[0009] Preferably, the promoting the growth of Solanaceae vegetables includes or improving the quality of seedlings under low temperature conditions; the temperature of the low temperature conditions is 10-15°C.

[0010] Preferably, the Solanaceae vegetables include one or more of tomatoes, peppers and eggplants.

[0011] The present invention also provides a method for improving stress resistance of Solanaceae vegetables and / or promoting growth of Solanaceae vegetables, comprising the following steps:

[0012] Solanaceous vegetable seeds are soaked in a seedling treatment solution for 6 to 24 hours, and then the soaked solanaceous vegetable seeds are dark-cultured for 72 to 120 hours to obtain dark-cultured seeds;

[0013] Sowing and germinating the dark-cultured seeds, and germinating the germinated Solanaceae seeds at 10-15° C. to obtain Solanaceae vegetable seedlings;

[0014] During the seedling emergence process, the seedlings are sprayed with the seedling treatment solution twice;

[0015] The seedling treatment liquid is prepared by dissolving the seedling treatment composition described in the above technical solution in water.

[0016] Preferably, the spraying time includes the 11th to 15th day and the 15th to 19th day after sowing, and the dosage of the seedling treatment liquid for each spraying is such that the droplets are evenly distributed on the leaves.

[0017] Preferably, the soaking temperature is 15-25°C; the germination temperature is 25°C, and the time is 3 days.

[0018] Preferably, the dark culture comprises: mixing the soaked Solanum seeds, vermiculite and water and then dark culturing, wherein the mass ratio of the Solanum seeds, vermiculite and water is 1:(1-2):(1-2); and the temperature of the dark culture is 15-20°C.

[0019] Beneficial effects:

[0020] The present invention provides a seedling treatment composition, comprising chitosan oligosaccharide, D-cellobiose and xylo-oligosaccharide, wherein the mass ratio of the chitosan oligosaccharide, D-cellobiose and xylo-oligosaccharide is (1-2): (1-2): (1-2). The present invention soaks Solanaceous vegetable seeds with a seed treatment solution prepared by dissolving the seedling treatment composition in water, and dark-cultures the soaked seeds, and sprays the seedling treatment solution during the Solanaceous vegetable seedling stage, which can effectively improve the low temperature resistance of Solanaceous vegetables, and improve the vitality of Solanaceous vegetable seeds and the quality of seedlings under low temperature conditions. The test results show that the combination of soaking Solanaceous vegetable seeds with a seedling treatment solution containing the seedling treatment composition, dark-culture and spraying the seedling treatment solution during the Solanaceous vegetable seedling stage can improve the seed germination potential, germination rate and germination index of Solanaceous vegetable seeds under low temperature conditions, shorten the average germination time, and at the same time, improve the chlorophyll content, plant height, root length and fresh weight of Solanaceous vegetable seedlings under low temperature conditions. It can be seen that the seedling treatment composition of the present invention can improve the germination characteristics of Solanaceae seeds, especially improve the germination ability of seeds under low temperature conditions, improve seed germination and seedling quality in the low temperature season in winter, and improve the stress resistance of seedlings. DETAILED DESCRIPTION

[0021] The invention provides a seedling treatment composition, comprising chitosan oligosaccharide, D-cellobiose and xylo-oligosaccharide, wherein the mass ratio of the chitosan oligosaccharide, D-cellobiose and xylo-oligosaccharide is (1-2): (1-2): (1-2).

[0022] The mass ratio of chitosan oligosaccharides, D-cellobiose and xylo-oligosaccharides described in the present invention is preferably 1:1:1. The relative molecular mass of the chitosan oligosaccharides described in the present invention is preferably <3000, more preferably 1000-2000; the degree of polymerization of the chitosan oligosaccharides is preferably 2-10; the chitosan with the relative molecular mass and degree of polymerization can activate the transcriptional expression of groups related to plant development, nitrogen metabolism, and carbon metabolism, and has the advantages of increasing plant biomass, promoting the growth of crop plants and roots, and improving resistance to biological and abiotic stresses. The relative molecular mass of xylo-oligosaccharides described in the present invention is preferably 300-1051, more preferably 300.28-1050.98; the xylo-oligosaccharides with the relative molecular mass have the advantage of promoting the growth of crop roots.

[0023] The dosage form of the seedling treatment composition of the present invention is preferably an aqueous solution, that is, the seed treatment composition is preferably a seed treatment solution, and the concentration of chitosan oligosaccharides in the seed treatment solution is preferably 0.04-0.08 g / L, and more preferably 0.04-0.06 g / L; the concentration of D-cellobiose in the seed treatment solution is preferably 0.04-0.08 g / L, and more preferably 0.04-0.06 g / L; the concentration of xylo-oligosaccharides in the seed treatment solution is preferably 0.04-0.08 g / L, and more preferably 0.04-0.06 g / L. The preparation method of the seed treatment solution of the present invention preferably comprises: dissolving the seed treatment composition in water to obtain the seed treatment solution.

[0024] The present invention also provides the use of the seedling treatment composition described in the above technical scheme in improving the stress resistance of Solanaceous vegetables and / or promoting the growth of Solanaceous vegetables. The seed treatment composition of the present invention is preferably a seed treatment solution, and the characteristics of the seed treatment solution have been defined in the above technical scheme and will not be repeated. The present invention preferably includes improving the stress resistance of Solanaceous vegetables and / or improving the low temperature resistance of Solanaceous vegetable seedlings under low temperature conditions. The present invention preferably includes improving the quality of seedlings under low temperature conditions; the improvement of seed vitality under low temperature conditions preferably includes improving the emergence potential, emergence rate, emergence index of seeds under low temperature conditions, and shortening the average emergence time. One or more; the improvement of the quality of seedlings under low temperature conditions preferably includes improving the quality of seedlings under low temperature conditions, and further preferably improving the chlorophyll content, plant height, root length and fresh weight of seedlings under low temperature conditions. More preferably, it is to improve the chlorophyll content, plant height, root length and fresh weight of seedlings under low temperature conditions. The temperature of the low temperature condition of the present invention is preferably 10-15°C, and more preferably 12-14°C. The solanaceous vegetables of the present invention preferably include but are not limited to one or more of tomatoes, peppers and eggplants.

[0025] The present invention also provides a method for improving stress resistance of Solanaceae vegetables and / or promoting growth of Solanaceae vegetables, comprising the following steps:

[0026] Solanaceous vegetable seeds are soaked in a seedling treatment solution for 6 to 24 hours, and then the soaked solanaceous vegetable seeds are dark-cultured for 72 to 120 hours to obtain dark-cultured seeds;

[0027] Sowing and germinating the dark-cultured seeds, and germinating the germinated Solanaceae seeds at 10-15° C. to obtain Solanaceae vegetable seedlings;

[0028] During the seedling emergence process, the seedlings are sprayed with the seedling treatment solution twice;

[0029] The seedling treatment liquid is prepared by dissolving the seedling treatment composition described in the above technical solution in water.

[0030] The present invention soaks the seeds of Solanaceae vegetables with a seedling treatment solution for 6 to 24 hours. The characteristics of the seed treatment solution of the present invention have been defined in the above technical scheme and will not be repeated here. The soaking time of the present invention is preferably 12 to 18 hours; the soaking temperature is preferably 15 to 25°C, more preferably 18 to 20°C. The Solanaceae vegetables of the present invention preferably include but are not limited to one or more of tomatoes, peppers and eggplants.

[0031] After the soaking, the present invention preferably drains the soaked seeds, rinses the soaked seeds with clean water, and dries them at room temperature to obtain dried seeds. The number of rinses in the present invention is preferably 3 times; the drying time is preferably 3 hours.

[0032] After obtaining the dried seeds, the present invention preferably mixes the dried seeds, vermiculite and water and then performs dark culture to obtain dark-cultured seeds. The mass ratio of the dried seeds, vermiculite and water in the present invention is preferably 1:(1-2):(1-2), and more preferably 1:1:(1.2-1.4). Vermiculite has a high porosity. The porous structure and expansion properties of vermiculite give it good adsorption, water absorption and moisture retention, and air permeability. It has a slow release and moisturizing effect, allowing the seeds to slowly absorb water and perform physiological and biochemical metabolism and repair of pre-germination. The temperature of the dark culture in the present invention is preferably 15-20°C, more preferably 16-18°C; the time of the dark culture is preferably 72-120h, and more preferably 84-96h. The dark culture in the present invention is preferably carried out in an incubator.

[0033] After obtaining the dark-cultured seeds, the present invention preferably separates the dark-cultured seeds from vermiculite and performs back-drying to obtain back-dried seeds. The back-drying temperature of the present invention is preferably 25 to 30° C., and the time is preferably 3 to 24 hours.

[0034] After obtaining the dried seeds, the present invention sows and germinates the dried seeds, and the germinated Solanaceae seeds are germinated at 10-15°C to obtain Solanaceae vegetable seedlings. The sowing of the present invention is preferably sown in a plug tray, and the type of matrix in the plug tray is not particularly limited. The conventional matrix used for plug tray sowing of Solanaceae vegetables in the art can be used. The germination temperature of the present invention is preferably 20-30°C, more preferably 25°C; the germination time is preferably 2-4 days, more preferably 3 days. In the process of germination of the present invention, the seedling treatment liquid is sprayed on the seedlings twice; the spraying time preferably includes the 11th to 15th day and the 15th to 19th day after sowing, more preferably the 11th day and the 15th day after sowing, or the 15th and the 19th day after sowing; the dosage of the seedling treatment liquid sprayed each time is preferably that the droplets are evenly distributed on the leaves. The invention has the advantage of spraying the seedling treatment solution on the 11th to 15th day and the 15th to 19th day after sowing in that it induces plant defense reactions and growth signals in the early seedling stage and activates and regulates metabolic activities related to plant growth and development.

[0035] In the actual planting process of Solanaceous vegetables, the present invention preferably also includes a subsequent management process for the Solanaceous vegetable seedlings. The present invention does not make any detailed limitations on the subsequent management process, which can be carried out according to conventional management measures in the art.

[0036] Treating Solanaceous vegetable seeds and seedlings separately based on the seed treatment composition of the present invention can improve the vitality of Solanaceous vegetable seeds; improve the seed vitality of Solanaceous vegetable seeds under low temperature conditions after sowing; improve the seed germination potential, germination rate, germination index of Solanaceous vegetable seeds under low temperature conditions after sowing and reduce the average seed germination time; improve the quality and stress resistance of Solanaceous seedlings under low temperature conditions.

[0037] In order to further illustrate the present invention, the technical solutions provided by the present invention are described in detail below in conjunction with embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0038] Example 1

[0039] A method for improving the low temperature resistance of Solanaceae vegetables and / or promoting the growth of Solanaceae vegetables, comprising the following steps:

[0040] 1) Preparation of seed treatment solution:

[0041] Chitosan oligosaccharide (purchased from Shanghai Yuanye Biotechnology Co., Ltd., CAS No.: 148411-57-8, product No.: S31060, the same as in the following examples), D-cellobiose (purchased from Shanghai Yuanye Biotechnology Co., Ltd., CAS No.: 528-50-7, product No.: S11030, the same as in the following examples) and xylo-oligosaccharide (purchased from Shanghai Yuanye Biotechnology Co., Ltd., product No.: S11137, the same as in the following examples) were dissolved in water to make the concentration of chitosan oligosaccharide 0.04 g / L, the concentration of D-cellobiose solution 0.04 g / L, and the concentration of xylo-oligosaccharide solution 0.04 g / L.

[0042] 2) Seed treatment and sowing:

[0043] Soak cherry tomato Jingfan 309 seeds in seed treatment solution at 20°C for 8 hours. After soaking, drain the water, rinse the seeds 3 times with clean water, and dry them at room temperature for 3 hours.

[0044] Add 1 g of the soaked seeds, 1 g of vermiculite and 1.2 g of water into a covered container, mix well, and place in a 20°C incubator in the dark for 72 h;

[0045] After the cultivation is completed, the seeds are separated from the vermiculite and slowly dried;

[0046] Sow the dried seeds in plug trays, germinate them at 25°C for 3 days, and then place them in a 15°C incubator for germination.

[0047] 3) Seedling treatment:

[0048] 14 days and 18 days after sowing, the seedlings were sprayed with the seed treatment solution prepared in step 1) twice, respectively, and the spraying amount each time was based on the droplets being evenly distributed on the leaves.

[0049] Comparative Example 1

[0050] Cherry tomato Jingfan 309 seeds were sown in plug trays, germinated at 25°C for 3 days, and then placed in a 15°C incubator for germination.

[0051] Example 2

[0052] A method for improving the low temperature resistance of Solanaceae vegetables and / or promoting the growth of Solanaceae vegetables, comprising the following steps:

[0053] 1) Preparation of seed treatment solution:

[0054] Chitosan oligosaccharide, D-cellobiose and xylo-oligosaccharide were dissolved in water, so that the concentration of chitosan oligosaccharide was 0.04 g / L, the concentration of D-cellobiose solution was 0.04 g / L, and the concentration of xylo-oligosaccharide solution was 0.04 g / L.

[0055] 2) Seed treatment and sowing:

[0056] Use seed treatment liquid to soak large tomato Pufen No. 1 seeds at 20℃ for 24 hours. After soaking, drain the water, rinse the seeds 3 times with clean water, and dry them at room temperature for 3 hours.

[0057] Add 1 g of the soaked seeds, 1 g of vermiculite and 1.4 g of water into a covered container, mix well, and place in a 20°C incubator in the dark for 120 h.

[0058] After the cultivation is completed, the seeds are separated from the vermiculite and slowly dried;

[0059] The dried seeds were sown in plug trays, germinated at 25°C for 3 days, and then placed in a 15°C incubator for germination.

[0060] 3) Seedling treatment:

[0061] 14 days and 18 days after sowing, the seedlings were sprayed with the seed treatment solution prepared in step 1) twice, respectively, and the spraying amount each time was based on the droplets being evenly distributed on the leaves.

[0062] Comparative Example 2

[0063] Sow the large tomato Pufen No. 1 in the plug tray, germinate at 25℃ for 3 days, and then place it in a 15℃ incubator for germination.

[0064] Example 3

[0065] A method for improving the low temperature resistance of Solanaceae vegetables and / or promoting the growth of Solanaceae vegetables, comprising the following steps:

[0066] 1) Preparation of seed treatment solution:

[0067] Chitosan oligosaccharide, D-cellobiose and xylo-oligosaccharide were dissolved in water, so that the concentration of chitosan oligosaccharide was 0.04 g / L, the concentration of D-cellobiose solution was 0.04 g / L, and the concentration of xylo-oligosaccharide solution was 0.04 g / L.

[0068] 2) Seed treatment and sowing:

[0069] Soak the Richie pepper seeds in seed treatment solution at 20°C for 24 hours. After soaking, drain the water, rinse the seeds 3 times with clean water, and dry them at room temperature for 3 hours.

[0070] Add 1 g of the soaked seeds, 1 g of vermiculite and 1.4 g of water into a covered container, mix well, and place in a 20°C incubator in the dark for 72 h.

[0071] After the cultivation is completed, the seeds are separated from the vermiculite and slowly dried;

[0072] Sow the dried seeds in plug trays, germinate them at 25°C for 3 days, and then place them in a 15°C incubator for germination.

[0073] 3) Seedling treatment:

[0074] 14 days and 18 days after sowing, the seedlings were sprayed with the seed treatment solution prepared in step 1) twice, respectively, and the spraying amount each time was based on the droplets being evenly distributed on the leaves.

[0075] Comparative Example 3

[0076] Sow the Rich Thin Pepper in a plug tray, germinate at 25°C for 3 days, and then place in a 15°C incubator for germination.

[0077] Example 4

[0078] A method for improving the low temperature resistance of Solanaceae vegetables and / or promoting the growth of Solanaceae vegetables, comprising the following steps:

[0079] 1) Preparation of seed treatment solution:

[0080] Chitosan oligosaccharide, D-cellobiose and xylo-oligosaccharide were dissolved in water, so that the concentration of chitosan oligosaccharide was 0.04 g / L, the concentration of D-cellobiose solution was 0.04 g / L, and the concentration of xylo-oligosaccharide solution was 0.04 g / L.

[0081] 2) Seed treatment and sowing:

[0082] Soak the Wild Wolf F1 eggplant seeds in the seed treatment solution at 20°C for 24 hours. After soaking, drain the water, rinse the seeds 3 times with clean water, and dry them at room temperature for 3 hours.

[0083] Add 1 g of the soaked seeds, 1 g of vermiculite and 1.4 g of water into a covered container, mix well, and place in a 20°C incubator in the dark for 72 h.

[0084] After the cultivation is completed, the seeds are separated from the vermiculite and slowly dried;

[0085] Sow the dried seeds in plug trays, germinate them at 25°C for 3 days, and then place them in a 15°C incubator for germination.

[0086] 3) Seedling treatment:

[0087] 14 days and 18 days after sowing, the seedlings were sprayed with the seed treatment solution prepared in step 1) twice, respectively, and the spraying amount each time was based on the droplets being evenly distributed on the leaves.

[0088] Comparative Example 4

[0089] Wild Wolf F1 eggplant seeds were sown in plug trays, germinated at 25°C for 3 days, and then placed in a 15°C incubator for germination.

[0090] Test Example 1

[0091] The emergence of the solanaceous vegetable seeds in Examples 1 and 2 was counted 5-15 days after sowing, and the quality of the solanaceous seedlings in Examples 1 and 2 was counted 26 days after sowing. The results are shown in Tables 1 and 2.

[0092] Table 1 Seedling situation of tomato seeds in Examples 1 to 2 at low temperature (15°C)

[0093]

[0094] Note: "*" in Table 1 indicates significant difference P < 0.05, the same as in Tables 2 to 4.

[0095] Table 2 Seedling quality of tomato seedlings at low temperature (15°C) in Examples 1 to 2

[0096]

[0097] Test Example 2

[0098] The emergence of pepper and eggplant seeds in Examples 3 to 4 was counted 5 to 27 days after sowing, and the quality of eggplant and pepper seedlings in Examples 3 to 4 was counted 27 days after sowing. The results are shown in Tables 3 and 4.

[0099] Table 3 Seedling situation of pepper and eggplant seeds in Examples 3 to 4 at low temperature (15°C)

[0100]

[0101] Table 4 Seedling quality of pepper and eggplant seedlings at low temperature (15°C) in Examples 3 to 4

[0102]

[0103]

[0104] It can be concluded from Tables 1 to 4 that after treating the seeds and seedlings of Solanaceous vegetables with the seedling treatment solution of the present invention, the stress resistance of Solanaceous vegetables can be improved, and the vitality of Solanaceous vegetable seeds under low temperature conditions and the quality of seedlings under low temperature conditions can be improved.

[0105] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A seedling treatment composition, characterized in that The invention comprises chitosan oligosaccharide, D-cellobiose and xylo-oligosaccharide, wherein the mass ratio of the chitosan oligosaccharide, D-cellobiose and xylo-oligosaccharide is (1-2): (1-2): (1-2).

2. The seedling treatment composition according to claim 1, characterized in that The relative molecular mass of the chitosan oligosaccharide is less than 3000, and the degree of polymerization is 2-10; the relative molecular mass of the xylooligosaccharide is 300-1051.

3. Use of the seedling treatment composition according to claim 1 or 2 in improving the stress resistance of Solanaceae vegetables and / or promoting the growth of Solanaceae vegetables.

4. The use according to claim 3, characterized in that: The method of improving stress resistance of Solanaceous vegetables includes improving the vitality of Solanaceous seeds under low temperature conditions and / or improving the low temperature resistance of Solanaceous vegetable seedlings.

5. The use according to claim 3 or 4, characterized in that: The method of promoting the growth of Solanaceae vegetables includes improving the quality of seedlings under low temperature conditions; the temperature of the low temperature conditions is 10-15°C.

6. The use according to claim 3, characterized in that: The solanaceous vegetables include one or more of tomatoes, peppers and eggplants.

7. A method for improving stress resistance of Solanaceae vegetables and / or promoting growth of Solanaceae vegetables, characterized in that: The steps include: Solanaceous vegetable seeds are soaked in a seedling treatment solution for 6 to 24 hours, and then the soaked solanaceous vegetable seeds are dark-cultured for 72 to 120 hours to obtain dark-cultured seeds; Sowing and germinating the dark-cultured seeds, and germinating the germinated Solanaceae seeds at 10-15° C. to obtain Solanaceae vegetable seedlings; During the seedling emergence process, the seedlings are sprayed with the seedling treatment solution twice; The seedling treatment liquid is prepared by dissolving the seedling treatment composition according to claim 1 or 2 in water.

8. The method according to claim 7, characterized in that The spraying time includes the 11th to 15th day and the 15th to 19th day after sowing, and the dosage of the seedling treatment liquid for each spraying is that the droplets fall naturally.

9. The method according to claim 7, characterized in that: The soaking temperature is 15-25° C.; the germination temperature is 25° C., and the time is 3 days.

10. The method according to claim 7, characterized in that The dark culture comprises: mixing the soaked Solanaceae seeds, vermiculite and water and then conducting dark culture, wherein the mass ratio of the Solanaceae seeds, vermiculite and water is 1:(1-2):(1-2); and the temperature of the dark culture is 15-20°C.

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