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Cowpea isolation hybridization pollination method

A technology for hybrid pollination and cowpea is applied in the field of isolated hybrid pollination of cowpea, which can solve the problems of bright color and large flowers, and achieves the effects of simple operation, reduction of false hybridization rate, and avoidance of self-crossing and natural hybridization.

Inactive Publication Date: 2008-01-23
成都市第一农业科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because cowpea is relatively strict self-pollination, but its flowers are large and colorful, and insects such as bees often collect pollination when it blooms, and some stigmas expose the keel when they first open the next morning. natural hybrid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Select suitable male parent and female parent to sow in the nutrient bowl respectively: select Chengjue No. 3 as the male parent and Zhiqing No. 3 as the female parent, because the seed coat of Chengjue No. 3 is black, and the seeds of Zhiqing No. 3 are red. Black is dominant relative to reddish brown, so the seed coat can be used as a marker trait. The pods produced by the first generation of hybridization should be black in seed color, which is easy to investigate the hybridization rate; transplanting, mulching, hole spacing 40cm, 2 plants per hole, management General production; during the flowering period, pollinate the healthy flower buds 1-3 days before flowering before 11:00 every day; investigate the hybridization rate after harvesting.

[0014] A, non-emasculated stigma isolation hybridization: select cowpea male parent and female parent with excellent traits, sowing and management are the same as general production; before 12 o'clock in the morning every day du...

Embodiment 2

[0021] Chengjue No. 3 was selected as the male parent, and Tezao 30 was selected as the female parent. The sowing and management were the same as in general production; during the flowering period, the strong flower buds 1-3 days before flowering were pollinated by emasculated and non-emasculated stigma hybridization methods; Afterwards, the hybridization rate was investigated.

[0022] Table 2 Effect of different pollination methods on pod setting rate of Tezao 30×Chengjue 3

[0023] different pollination methods

[0024] The experimental results showed that the pod setting rate, pod index and hybridization rate of non-emasculated stigma hybridization method were better than those of emasculated hybridization method.

Embodiment 3

[0026] Select the male parent and female parent with excellent traits, sowing and management are the same as general production; during the flowering period, the non-emasculating stigma isolation hybridization method is used to pollinate the robust flower buds 1 day before flowering, 2 days before flowering, and 3 days before flowering; The hybridization rate was investigated after harvesting. The results are shown in Table 3. The highest pod setting rate was 1 day before flowering, up to 20.37%, which was 42.54% higher than the previous 2 days, and 322.61% higher than the previous 3 days; followed by the first 2 days, the pod setting rate was 14.29%. , 196.47% higher than the previous 3 days; the lowest pod setting rate was pollination 3 days before flowering, only 4.82%. It shows that different pollination time is one of the important factors affecting the pod setting rate, and the best time is 1 day before flowering.

[0027] Table 3. Effect of different pollination time o...

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Abstract

The invention discloses a cowpea isolating hybridization pollination method. The technical solution is that: choosing male parent and female parent of cowpea with excellent performance; selecting hale anthotaxy bud 1 to 3 days before the female parent flower open and suiting their necking with partition to separate the gynoecia from the androecium; collecting farina of the unopened male parent in a farina box for standby and then place the farina of the male parent into the partition for pollination on the next day of isolating the necking to complete the hybridization and marked with labels. The invention saves the castration procedure, which features simple operation, time and labor-saving and free of damaging the flower's natural structure and affecting the normal growth conditions for germ cell with high breeding success rates; besides, the invention, by applying the partition to suit the necking to isolating the gynoecia and the androecium, avoids the self cross and hybridization and effectively reduces the fake hybridization rate.

Description

technical field [0001] The invention relates to the technical field of plant cross-breeding, in particular to a method for isolated cross-pollination of cowpea. Background technique [0002] Due to the flowering and fruiting characteristics of bean crops, the hybridization work is relatively difficult, so that the breeding of new varieties lags behind. There are two kinds of existing cowpea hybrid breeding methods, one method is emasculated hybridization, and the other method is non-castrated stigma hybridization. Castration hybridization is to peel off the sepals and petals manually a few days before the flowering of the female parent, and remove the stamens with tweezers to prevent self-fertilization. This hybridization method can ensure the purity of hybridization; , The keel petals are curved inward or arched, the petals are tightly interlocked, and the style is long. When the petals are peeled off and the stamens are removed, the natural structure of the flower will in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 陈玲袁水全先本刚
Owner 成都市第一农业科学研究所
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