Corn haploid inducing method

Abstract

The invention discloses a corn haploid inducing method. The method comprises the steps of pollinating a female parent by using a corn haploid inducing system 'qiyou 201' as a male parent and harvesting current-generation hybridized grains to obtain corn haploids, wherein the planting modes of the female parent and the male parent are as follows: the female parents and the male parents are planted alternatively in an isolating zone in a row manner, and are sown in different stages to enable the female parents and the male parents to realize flower synchronization which is characterized in that when 3-5cm of filaments of the female parent grow, the male parent starts to perform pollen application, the female parent is subjected to castration before pollen application of the male parent, and subtending leaves of the female parent are cut off when the male parent performs pollen application. Experiments show that the corn haploid inducing method has the advantages that the corn haploid inducing rate is up to be more than 19% compared with the corn haploid inducing rate of the traditional method, the high-efficiency haploid inducing system 'qiyou 201' has strong growth vigor, is mid-maturation, and has high pollinating amount, long pollen dispersing duration time, wide adaptability and strong resistance, solves the technical bottleneck of low inductivity of the traditional haploid induction system and provides technical support for large-scale haploid induction in commercialized corn breeding.

Description

technical field [0001] The invention relates to a maize haploid induction method. Background technique [0002] Maize is a monoecious, sexually reproducing crop. The main way to increase maize yield is to use maize heterosis. The focus of maize breeding work is to develop maize hybrids. Breeding maize inbred lines is the basis for cultivating maize hybrids; through crossing between inbred lines to prepare maize combinations and breed new maize hybrids. Therefore, the technology of breeding maize inbred lines is the core technology of maize breeding. The traditional breeding technology of maize inbred lines requires many years of multi-generation self-selection and combining ability measurement technology to breed inbred lines with high combining ability and stable traits; and then breed new maize hybrids through crossbreeding . Breeding a new corn hybrid usually takes more than ten years, which is not only a long period, but also consumes a lot of manpower, material res...

AI Technical Summary

Problems solved by technology

[0006] There are two major defects in the technology of using Stock6 as the induction line and hybridization to induce haploids in maize: one is that the induction rate is low (about 2%), and it is difficult to meet the needs of large-scale induction of haploids

However, the performance of biological characteristics is still unsatisfactory, especially poor powder loosening, long time interval between flowering and silking, poor self-fertilization, difficulty in self-propagation, severe ear and grain rot, etc., which are difficult to meet the requirements of commercial breeding. Scale-induced haploid requirement

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