Corn haploid inducing method

A technology of haploid induction and haploid induction system, which is applied in the fields of botanical equipment and methods, application, plant gene improvement, etc., can solve problems such as poor powder loosening, low induction rate, and severe ear and grain rot, and achieves Long duration of loose powder, strong plant growth and wide adaptability

Inactive Publication Date: 2014-08-27
MAIZE RES INST SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There are two major defects in the technology of using Stock6 as the induction line and hybridization to induce haploids in maize: one is that the induction rate is low (about 2%), and it is difficult to meet the needs of large-scale induction of haploids
However, the performance of biological characte

Method used

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  • Corn haploid inducing method
  • Corn haploid inducing method
  • Corn haploid inducing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1, analysis of biological characteristics of corn (Zea mays L.) Qiyou 201

[0037] Maize (Zea mays L.) was planted in 4 rows at the Shandong Academy of Agricultural Sciences (61) experimental station in summer, and its biological characteristics were analyzed. The agronomic traits investigated included: plant height, ear height, Loose powder period, spinning period, etc.

[0038] Plant height: Plant height refers to the distance from the ground to the top of the tassel of the plant.

[0039] Ear height: Ear height refers to the distance from the ground to the base of the ear.

[0040] Pollination period: The pollination period refers to the date when the middle part of the tassel begins to loose pollen, and 60% of the plants in each row are defined as the pollination period.

[0041] The number of days from sowing to loose powder: refers to the number of days from sowing to loose powder.

[0042] Silking stage: The silking stage refers to the silking stage when...

Embodiment 2

[0053] Embodiment 2, the condition optimization of maize haploid induction

[0054] In this example, corn (Zea mays L.) Qiyu 201 was used as the male parent to induce maize haploids, and the planting method of the male parent and the female parent was as follows: the male parent and the female parent were alternated in rows Planted in the isolated area, staggered sowing so that the flowering periods of the two meet, and the female parent is detasseled before the male parent is dusted. Among them, two important parameters, the way the parents planted in rows and the time of staggered planting, were optimized.

[0055] 1. Optimizing the planting method of parents in rows

[0056] Female parent: maize (Zea mays L.) X01.

[0057] The test was arranged in order, and the male parent and the female parent were planted alternately in rows, and a total of 32 rows were planted, as follows:

[0058] Since the male parents must be planted in the edge rows of the isolated area, two rows...

Embodiment 3

[0112] Embodiment 3, the application of maize haploid induction

[0113] In this example, maize (Zea mays L.) Qiyu 201 was used as the male parent, and maize (Zea mays L.) X01, X02, X03, X04, X05, X06 and X07 were used as the female parents to induce haploids in maize .

[0114] The experiment adopts random block arrangement, and the male parent and the female parent are planted in the isolated area alternately in rows (4 rows of female parents are planted, followed by 2 rows of male parents). When the filaments were drawn out by 3 to 5 cm, the male parent began to dust), and before the male parent was dusted, the female parent was detasselled, and when the male parent was dusted, the female parent was cut. Natural cross-pollination with the female parent.

[0115] After the experiment, 50-80 hybrid ears were randomly selected from each treatment, and the haploid induction rate was calculated according to the number of hybrid kernels and the number of haploid kernels among t...

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Abstract

The invention discloses a corn haploid inducing method. The method comprises the steps of pollinating a female parent by using a corn haploid inducing system 'qiyou 201' as a male parent and harvesting current-generation hybridized grains to obtain corn haploids, wherein the planting modes of the female parent and the male parent are as follows: the female parents and the male parents are planted alternatively in an isolating zone in a row manner, and are sown in different stages to enable the female parents and the male parents to realize flower synchronization which is characterized in that when 3-5cm of filaments of the female parent grow, the male parent starts to perform pollen application, the female parent is subjected to castration before pollen application of the male parent, and subtending leaves of the female parent are cut off when the male parent performs pollen application. Experiments show that the corn haploid inducing method has the advantages that the corn haploid inducing rate is up to be more than 19% compared with the corn haploid inducing rate of the traditional method, the high-efficiency haploid inducing system 'qiyou 201' has strong growth vigor, is mid-maturation, and has high pollinating amount, long pollen dispersing duration time, wide adaptability and strong resistance, solves the technical bottleneck of low inductivity of the traditional haploid induction system and provides technical support for large-scale haploid induction in commercialized corn breeding.

Description

technical field [0001] The invention relates to a maize haploid induction method. Background technique [0002] Maize is a monoecious, sexually reproducing crop. The main way to increase maize yield is to use maize heterosis. The focus of maize breeding work is to develop maize hybrids. Breeding maize inbred lines is the basis for cultivating maize hybrids; through crossing between inbred lines to prepare maize combinations and breed new maize hybrids. Therefore, the technology of breeding maize inbred lines is the core technology of maize breeding. The traditional breeding technology of maize inbred lines requires many years of multi-generation self-selection and combining ability measurement technology to breed inbred lines with high combining ability and stable traits; and then breed new maize hybrids through crossbreeding . Breeding a new corn hybrid usually takes more than ten years, which is not only a long period, but also consumes a lot of manpower, material res...

Claims

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Application Information

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IPC IPC(8): A01H1/02
CPCA01H1/08
Inventor 刘治先杨菲刘长虹
Owner MAIZE RES INST SHANDONG ACAD OF AGRI SCI
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