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A kind of maize haploid induction method

A technology of haploid induction and haploid induction system, which is applied in the fields of botanical equipment and methods, application, plant gene improvement, etc., can solve problems such as poor powder loosening, low induction rate, and severe ear and grain rot, and achieve Long duration of loose powder, strong plant growth and wide adaptability

Inactive Publication Date: 2016-06-01
MAIZE RES INST SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There are two major defects in the technology of using Stock6 as the induction line and hybridization to induce haploids in maize: one is that the induction rate is low (about 2%), and it is difficult to meet the needs of large-scale induction of haploids
However, the performance of biological characteristics is still unsatisfactory, especially poor powder loosening, long time interval between flowering and silking, poor self-fertilization, difficulty in self-propagation, severe ear and grain rot, etc., which are difficult to meet the requirements of commercial breeding. Scale-induced haploid requirement

Method used

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  • A kind of maize haploid induction method
  • A kind of maize haploid induction method
  • A kind of maize haploid induction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1, analysis of biological characteristics of corn (Zeamays L.) Qiyou 201

[0037]At the experimental station of Shandong Academy of Agricultural Sciences (61) in summer, 4 rows of corn (Zeamays L.) were planted in Qiyu 201, and its biological characteristics were analyzed. , spinning period, etc.

[0038] Plant height: Plant height refers to the distance from the ground to the top of the tassel of the plant.

[0039] Ear height: Ear height refers to the distance from the ground to the base of the ear.

[0040] Pollination period: The pollination period refers to the date when the middle part of the tassel begins to loose pollen, and 60% of the plants in each row are defined as the pollination period.

[0041] The number of days from sowing to loose powder: refers to the number of days from sowing to loose powder.

[0042] Silking stage: The silking stage refers to the silking stage when the female ear silks are spit out about 2 cm, and when 60% of the plants ...

Embodiment 2

[0053] Embodiment 2, the condition optimization of maize haploid induction

[0054] In this example, corn (Zeamays L.) Qiyu 201 was used as the male parent to induce maize haploid, and the planting method of the male parent and the female parent was: the male parent and the female parent were planted in alternate rows In the isolated area, sowing at the wrong time makes the two flowering periods meet, and the female parent is detasselled before the male parent is dusted. Among them, two important parameters, the way the parents planted in rows and the time of staggered planting, were optimized.

[0055] 1. Optimizing the planting method of parents in rows

[0056] Female parent: maize (Zeamays L.) X01.

[0057] The test was arranged in order, and the male parent and the female parent were planted alternately in rows, and a total of 32 rows were planted, as follows:

[0058] Since the male parents must be planted in the edge rows of the isolated area, two rows of male parent...

Embodiment 3

[0112] Embodiment 3, the application of maize haploid induction

[0113] In this example, corn (Zeamays L.) Qiyu 201 was used as the male parent, and corn (Zeamays L.) X01, X02, X03, X04, X05, X06 and X07 were used as the female parents respectively to induce maize haploid.

[0114] The experiment adopts random block arrangement, and the male parent and the female parent are planted in the isolated area alternately in rows (4 rows of female parents are planted, followed by 2 rows of male parents). When the filaments were drawn out by 3 to 5 cm, the male parent began to dust), and before the male parent was dusted, the female parent was detasselled, and when the male parent was dusted, the female parent was cut. Cross-pollination with the female parent naturally.

[0115] After the experiment, 50-80 hybrid ears were randomly selected from each treatment, and the haploid induction rate was calculated according to the number of hybrid kernels and the number of haploid kernels am...

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Abstract

The invention discloses a maize haploid induction method. The method is to use the maize haploid induction line "Qiyu 201" as the male parent to pollinate the female parent, harvest hybrid contemporary seeds, and obtain maize haploid; wherein, the planting methods of the male parent and the female parent are: The male parent and the female parent are planted alternately in the isolation area in rows, sowing at the wrong time so that the two flowering periods meet (the male parent begins to sprinkle powder when the female parent's filaments are drawn out by 3 to 5 cm), and the male parent is sprinkled with powder The female parent is detasselled before, and the bract leaves are cut when the male parent is dusted. Experiments have proved that compared with the traditional method, the maize haploid induction rate of the invention is as high as 19%. "Qiyu 201", a high-efficiency haploid induction line, has strong plant growth, medium maturity, large amount of loose powder, long duration of loose powder, wide adaptability and strong resistance. It solves the technical "bottleneck" of low induction rate of traditional haploid induction lines, and provides technical support for large-scale induction of haploids in commercial maize breeding.

Description

technical field [0001] The invention relates to a maize haploid induction method. Background technique [0002] Maize is a monoecious, sexually reproducing crop. The main way to increase maize yield is to use maize heterosis. The focus of maize breeding work is to develop maize hybrids. Breeding maize inbred lines is the basis for cultivating maize hybrids; through crossing between inbred lines to prepare maize combinations and breed new maize hybrids. Therefore, the technology of breeding maize inbred lines is the core technology of maize breeding. The traditional breeding technology of maize inbred lines requires many years of multi-generation self-selection and combining ability measurement technology to breed inbred lines with high combining ability and stable traits; and then breed new maize hybrids through crossbreeding . Breeding a new corn hybrid usually takes more than ten years, which is not only a long period, but also consumes a lot of manpower, material res...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
CPCA01H1/08
Inventor 刘治先杨菲刘长虹
Owner MAIZE RES INST SHANDONG ACAD OF AGRI SCI
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