Gene for controlling rice fertility, encoded protein and application thereof

A fertility gene, rice technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problems of low efficiency, no ABCG15, single population genetic type and trait expression, etc., to achieve labor saving, effective methods, and expansion. germplasm-based effects
CN102634522AActive Publication Date: 2012-08-15SICHUAN AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SICHUAN AGRI UNIV
Publication Date
2012-08-15

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Abstract

The invention discloses a gene ABCG15 for controlling rice fertility, an encoded protein and the application thereof. The invention also discloses a single recessive nuclear sterile gene, which is generated by mutation caused by deletion of the gene 12bp for controlling the rice fertility. The invention also discloses a method for obtaining a rice sterile line by controlling the expression of the rice fertility gene according to an RNAi technology and the method comprises the following steps of: amplifying a DNA fragment for generating an anti-sense RNA, constructing an expression vector, and transforming the DNA fragment into the normal fertile rice, so as to obtain a new rice nuclear sterile material. The newly found gene for controlling the rice fertility provides a new way for culturing the rice nuclear sterile line. The method for generating the rice nuclear sterile line is convenient and quick. The rice nuclear sterile material can be used for replacing the manual castration during the rice hybridization, labor is saved; the rice nuclear sterile material also can be used for recurrent selective breeding and plays an important role in widening the rice germ plasm basis.
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Description

technical field

[0001] The invention belongs to the field of plant fertility genes. It specifically relates to a gene and its encoded protein for controlling the fertility of rice; and a single recessive nuclear sterility gene produced by mutation of the gene; in addition, it also involves the use of RNAi technology to control the expression of the rice fertility gene to obtain rice fertility Breeding method Background technique

[0002] Rice is a self-pollinated crop. For a long time, the improvement of rice varieties has mainly relied on hybrid breeding methods. However, due to the limitations of artificial castration and hybridization techniques, usually only a small number of parents can be used for hybridization. Difficult to breed breakthrough breeds. The main agronomic traits of rice are mostly quantitative traits controlled by micro-effect multi-genes. Recurrent selection breeding method can break the linkage of unfavorable genes and increase the accumulation of go...

Claims

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