High-efficiency pea cross breeding method

Abstract

The invention discloses a high-efficiency pea cross breeding method. The high-efficiency pea cross breeding method comprises the following steps of: (1) performing castration, namely selecting a bud to bloom from a female parent plant, holding the torus lightly with the hand, clamping the position 3.5 to 4.5 cm away from the top of vexil and the connecting position of the vexil and the torus by using forceps, and clamping and pulling out petals, wherein the included angle of the forceps and the bud back line is 40 degrees; (2) performing pollination, namely selecting a male patent flower withpollen to be scattered or scattered just now, taking out pollen grains to place into a culture dish, clamping the pollen grains scattered just now by using the forceps, and coating the pollen on the tomentum of an exposed stigma of the female parent flower; (3) fastening a label; and (4) pruning. By the method, all the petals are pulled out at one step by the forceps, so the castration speed is high and the castration is performed completely; the stigma is not damaged; the working efficiency is three times higher than that of the traditional method and two times higher than that of a Suzonganmethod; success rate of hybrid fructification is increased by 33 percent compared with that of the traditional method and is increased by 13 percent compared with that of the Sizongan method; and theoccurrence probability of descendant pseudo hybrid is 0.

Description

technical field [0001] The invention relates to a pea hybrid breeding method. Background technique [0002] Pea is a relatively strict self-pollination crop, usually before the florets open, the anthers have dehiscated to complete the self-pollination process. In the pea hybrid breeding process, detasseling is one of the important steps in pea hybridization. At present, the classic method of pea hybridization is that after selecting suitable hybrid flowers, peel off the flag petals, wing petals and keel petals with tweezers, and then take out 10 pollen grains one by one, and then take pollen on the stigma of the pistil. Traditional method of emasculation of peas to restore flower buds. This method has low efficiency of emasculation and hybridization, and is likely to cause consequences such as incomplete emasculation, easy damage to the stigma of the female parent during emasculation and pollination, resulting in failure of hybridization. In 2007, Su Zong'an and others di...

AI Technical Summary

Problems solved by technology

The advantage of this method is that it is easier to pollinate and will not damage the stigma of the female parent. However, since this method does not detassell the female flower, the probability of pseudo hybrids in the hybrid offspring is higher.

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