Fluorescent staining method for evaluating sperm motility of domesticated mammals

A sperm motility and fluorescent staining technology, applied in the field of fluorescent staining, can solve the problems of limited use, expensive SYBR-14 probe, spectral overlap, etc., and achieve the effect of reducing the detection cost
CN104498583AInactive Publication Date: 2015-04-08YUNNAN AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
YUNNAN AGRICULTURAL UNIVERSITY
Publication Date
2015-04-08
Estimated Expiration
Not applicable · inactive patent
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Abstract

The invention discloses a fluorescent staining method for evaluating sperm motility of domesticated mammals. The fluorescent staining method comprises the following step: according to the characteristic that a mammal live sperm probe Hoechst 333422 and a mammal dead sperm probe PI can be excited by the same ultraviolet light to emit fluorescent light, performing fluorescent staining on sperms of four important domesticated mammals which are pigs, cows, sheep and dogs to accurately perform sperm mobility evaluation. Compared with the conventional PI / SYBR-14 staining method, the PI / Hoechst 33342 staining method adopts Hoechst 33342 to substitute an expensive SYBR-14 probe, so that the detecting cost is greatly lowered. The fluorescent staining method can be used for successfully evaluating the sperm mobility of important domesticated mammals such as pigs, cows, sheep and dogs, and can be widely applied to evaluating the sperm motility of various domesticated mammals.
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Description

technical field

[0001] The invention belongs to the technical field of animal reproduction, and in particular relates to a fluorescent staining method for evaluating sperm motility of several important domesticated mammals. Background technique

[0002] Sperm motility (also known as sperm plasma membrane integrity) evaluation is an essential index in the detection of sperm quality. The detection methods mainly include ordinary staining, fluorescent staining and hypotonic swelling test. Although the hypotonic swelling test is simple to operate , The price is low, but it is rarely used now because of the single detection index and the inability to analyze by flow cytometry.

[0003] Dyes commonly used in common dyeing methods include eosin, eosin-nigrosin, Giemsa, typan blue, Bismarck brown Y, Rose Bengal, etc. . The dyeing methods used include single dyeing method using only one dye, and double dyeing or triple dyeing method using two or more dyes at the same tim...

Claims

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