46 results about "Anti fertility" patented technology

Methods and compositions for inhibiting or preventing spermatogenesis in a male mammal. Also disclosed are compounds and formulations for use in such methods.

Pharmaceutical compositions for treating male sub-fertility that include an agent that causes a reduction in an effect of extracellular DNA on sperm cells, are provided. The agent may be, for example, an enzyme that degrades DNA such as DNase, a substance that blocks the interaction between cell free DNA and sperm cell surface receptors, a substance that binds to DNA, a substance that inhibits endogenous sperm cell DNase, a substance that inhibits a member of a signal transduction pathway mediated by DNA binding to sperm cell surface receptors, or an agent that stimulates production of an endogenous substance that causes a reduction in an antifertility effect of cell free DNA on sperm cells. Also provided are methods for treating male sub-fertility by administering the pharmaceutical composition to a subject in need thereof. Further provided are methods for determining a fertility status in a male subject, methods for assisted reproduction, methods for selecting an assisted reproduction technique (ART), and methods for selecting sperm cells in a sperm cell population for use in an assisted reproduction technique.

The invention discloses an epididymis protease inhibitor which is a functional peptide segment of Eppin. The inhibitor is any one selected from amino acid sequences shown from SEQ ID No.1 to SEQ ID No.7 and has advantages of single component, simple structure and strong pertinence of immunoreaction inspired; polypeptide with at least 80 percent of sequences the same as the functional peptide segment of Eppin can be used for constructing immunogen which can improve the immunogenicity of the functional peptide segment of Eppin, stimulate an organism to generate a specific antibody, reduce Eppin level in blood plasma and induce high-efficient, safe and reversible antifertility effect. The immunogen of the epididymis protease inhibitor can form a medicament composite with medically acceptable carriers, and the composite can be used for preparing male contraception vaccines, therefore, the epididymis protease inhibitor has broad application prospect.

The invention discloses an epididymal protease inhibitor protein monoclonal antibody and application thereof, which belong to the field of biological engineering. The monoclonal antibody is secreted by hybridoma preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of C200849. The monoclonal antibody can be combined to the epididymal protease inhibitor protein Eppin protein of specific expression in a testis and epididymis tissue by specificity. The monoclonal antibody can be used for preparing male infertility diagnostic reagents, male contraceptives and anti-fertility study biological products.

The invention discloses two optimization methods for attenuated processing of tripterygium wilfordii and the relevant preparations thereof. The optimization method (1) is that under the optimized condition, the traditional Chinese medicine tripterygium wilfordii is processed with salt water (or processed with acid) and processed with the acid (or processed with the salt water), or acid solution containing chloride ion is used for attenuation; the optimization method (2) comprises the following steps: under the optimized condition, tripterygium wilfordii extract, or the further purified product of the tripterygium wilfordii extract, or tripterygium wilfordii preparation is processed with the salt water (or processed with the acid) and processed with the acid (or processed with the salt water), the detailed method is described in the specifications. The methods have the advantages that the relevant preparations of the tripterygium wilfordii with the relatively low toxicity are obtained by the optimization of processing method, the medicinal resources of herb, root and bark, stem leaf parts of the tripterygium wilfordii with strong toxicity are utilized better, and the curative effect of tripterygium wilfordii preparations is strengthened. The methods of the invention simultaneously have the advantages of simple operation, low cost and being easy for popularization. The relevant preparations of the invention are used for treating rheumatoid arthritis, chronic nephritis, hepatitis, thrombopenic purpura and various dermatoarthritis, and can be used for resisting generation, anti-tumor, resisting rejection in the body after organ transplantation, etc.

The invention discloses a kind of ant bearing mouse trap agent, its use and its formula to kill and control the mouse breed. It contains the phosphine oxide aziridine barren medicament, the dait agent and rop findings, the main element, phosphine oxide aziridine can cause gland hurt for female mouse, the sperm occur barrier, at the same time, hae outstanding breed toxicity for female mouse, and can pass through the embryo and latex to transmit to descendant, seriously affect the bearing ability of descendant mouse. The mouse trap can escape the secondary hazarded phenomenon that bring by the traditional mouse trap, at the same time, the mouse cannot appearance the drastic reaction, such as bleed and convulsion after take in the mouse trap solvent, cannot cause awake of mouse, even more appearance the quickly rebound phenomenon of mouse group number after population vacuum, thus, it is a kind of biology ant bearing mouse trap agent that is safe and efficient, can use long period.

The invention discloses a grass meal granular formulation for controlling rodent pests in grassland and a preparation method as well as application thereof. The preparation method comprises the following steps of: preparing a neem seed extractive, then manufacturing the neem seed extractive, grass meal granules and an auxiliary material into the granular formulation according to a certain proportion, wherein the mass proportion of the neem seed extractive, the grass meal granules and the auxiliary material is 2-4:2-4:1-2. The preparation technology provided by the invention is simple, reliable and easy to control; and the prepared grass meal granular formulation is fragrant in flavor, has the good palatability and obvious anti-fertility effect on male and female plateau pika and is a novel botanical bisexual sterility rodenticide for controlling the rodent pests in the grassland.

The present invention discloses a quinazol derivative of a structural formula I, comprising the 3-substituted-4(3H)-quinazol derivative (compound 1-8) and the 6- substituted-4(3H)-quinazol derivative (compound 9-27). By testing each of the target compounds of the present invention in vitro inhibition of human sperm acrosin activity, the result shows that, compared with the publically reported acrosin inhibitor TLCK (N-toluene sulfonyl-L-lysyl-chloromethyl ketone), all target compounds have inhibit activity to human sperm acrosin. The quinazol derivative can be applied for preparing anti-fertility drugs and providing a new male contraceptive medicine in clinical field, and has a great application value. The present invention further provides a preparation method of the derivative I.

The invention discloses steroidal saponin derivatives, and a preparation method and application thereof. According to the invention, pseudoprodioscin is used as a substrate; a Chaetomium olivaceum strain is used as a biotransformation bacterium for biotransformation; and separation is finally carried out so as to obtain the steroidal saponin derivatives as shown in formula I to formula V in the specification. Pharmacological experiments prove that the steroidal saponin derivatives as shown in formula I to formula V have excellent antineoplastic activity and thrombolytic activity, substantial protection activity to myocardial cells and low toxicity to normal cells; so the steroidal saponin derivatives have important application prospects in cancer prevention, treatment of the diseases of cardiovascular systems, improvement of immunity, reduction in blood sugar, anti-fertility action, bacterium resistance, etc.

The invention provides a dehydroandrographolide glycine semi-ester hydrochloride derivative composition. The composition comprises dehydroandrographolide glycine semi-ester hydrochloride, a metal complexing agent and / or other pharmaceutically acceptable carriers. The invention also provides a preparation method as well as application of the composition. The composition can be used for adjusting the nonspecific immunity function, and has anti-tumor, anti-pyretic, anti-inflammatory, anti-virus, liver protection, cholagogic, anti-diabetic, anti-fertility and cardiovascular protection effects. The purity of the active ingredient of the composition is high, and the risk of adverse effect caused by impurities is avoided; the composition is in a scientific proportion, so that the stability of the medicament is greatly improved and other serious adverse events caused by degradation and polymer formation occurring in manufacturing and storage of the medicament are avoided; the composition has a better curative effect and an excellent effect on the adjustment of the nonspecific immunity function.

The utility model relates to a whole human anti follicule-stimulating hormone (FSH) Beta single chain antibody. Phage antibody that contains anti-A epitope antibody genes is screened out from the whole human single chain phage antibody library and conducted soluble expression to get the whole human single chain antibody of anti FSH-Beta A epitope; the A epitope is the 33-53 amino acid residue on FSH-Beta subunit and comprises the amino acid sequence of Glu-Glu-Cys-Arg-Phe-Cys-Ile-Ser-Ile-Asn-Thr-Thr-Trp-Cys-Ala-Gly-Tyr-Cys-Try-Thr-Arg. The utility model also relates to a method that utilizes phage display technology to screen the whole human anti FSH-Beta single chain antibody. The whole human anti FSH-Beta single chain antibody of the utility model can be applied to the purposes of anti-fertility, detection kit preparation and relevant diseases treatment.

The invention discloses a method of using embelia laeta to prepare bisexual antifertility material embelin, which mainly includes the steps: drying ripe embelia laeta, crushing into powder, adding water soluble cosolvent, stirring, extracting, filtering, extracting dregs for five to ten times, combining filtrate, diluting the filtrate for standing by to enable orange-yellow crystal to seed out, centrifugally separating, washing with water and vacuum drying to obtain an embelin crystal product. The embelin has various pharmacological activities such as blood sugar reduction, islet cell protection, liver injury prevention, oxygenated free radical resistance, osteoproliferation prevention, convulsion proofing, middle cerebral artery occlusion improvement, cerebral anoxia protection, myocardial infarction prevention, wound healing promotion, oncogene inhibition, liver cancer prevention, cholinesterase inhibition, amylase activity inhibition, abirritation, ulcerative colitis prevention and the like except for the antifertility function. The method has the advantages that 1, the embelin obtained by the method is higher than 90% in purity, higher than 95% in extraction rate and 2.4-3.0% in yield; 2, the preparation conditions are simple, no organic solvent but only the water soluble cosolvent (reusable), a stirrer and a centrifuge are needed, and environment pollution is avoided; and 3, the method is technically mature, high in competitiveness, free of 'waste gas, waste water and industrial residue', and bright in industrial prospect.

The invention discloses 1-(substituted benzoyl)-indazole-3-carboxylate or amide compounds A thereof. The compounds have strong antifertility activity so as to be used in preparing antifertility medicine and antivirus medicine. The invention provides novel clinic male prophylactic and antivirus medicine with large application value, the invention also provides preparing methods thereof.

The invention discloses an epididymis protease inhibition protein B-cell epitope and a multiple antigenic peptide containing the epitope; the epididymis protease inhibition protein B-cell epitope of the invention has amino acid sequences shown in SEQ ID No.1 and has single component and simple structure, and is free of the impact of bad epitopes in natural proteantigens, and immunoreactions initiated by the epitope of the invention has strong pertinence; the multiple antigenic peptide containing the epididymis protease inhibition protein B-cell epitope, is obtained by connecting a T accessory cell epitope with the epididymis protease inhibition protein B-cell epitope on a branch of a tree-based core formed by a bifunctional unit or a plurality of bifunctional units; the multiple antigenic peptide can induce that an antibody of the epididymis protease inhibition protein B-cell epitope is targeted with high titer in a specific manner and a reversible anti-fertility function with high efficiency and safety is produced, thereby being capable of preparing a male pregnancy vaccine, having significant social and economic benefits and having important value in theory and wide application prospect.

The invention discloses six immune anti-fertility polypeptide segments, all of which have excellent immunogenicity. The six immune anti-fertility polypeptide segments can stimulate the organisms to generate specific antibodies and induce the anti-fertility function. According to the fertility comparison of antigen polypeptide immunized mice and the immunized mice in a control group (KLH (keyhole limpet homocyanin) control group), immunization by the six polypeptide antigens mixed group can significantly reduce the farrowing rate of the male and the female mice, wherein litter sizes in the male and the female mice is respectively reduced by 62.8% and 50%. The athletic ability and activity of sperms are significantly reduced in the HE6 polypeptide group or the mixed group immunized male mice. The polypeptide with high-efficiency anti-fertility effect establishes the foundation for research of the mouse-specific immune anti-fertility vaccines, and can be used for application of various anti-fertility preparations.

Disclosed herein are non-synthetic herbal based anti-fertility compositions having high spermicidal activity for intravaginal administration comprising hydroalcoholic extract of Annona squamosa and pharmaceutically acceptable excipients, in suitably formulated dosage forms for intravaginal administration and a method of contraception in a female subject to prevent pregnancy.

The present invention relates to a kind of new-type tetrahydroisoquinoline compound with antifertility activity and antifungal activity and its pharmaceutically-acceptable salt. Said compound can be used for preparing antifertility medicine and antifungal medicine. Said invention also provides the structure general formula of said compound.

The invention discloses the application of sanguinarine and its salt as external spermicide for women. Its notable feature is that it can kill sperm quickly with low concentration, and it is cheap, safe and reliable. The minimum spermicidal concentration of sanguinarine aqueous solution (pH=4.5) was 0.25 mg / L for 20 seconds, and 0.15 mg / L for 3 minutes. The low spermicidal concentration in the anti-fertility test was 0.5 mg / kg with an ED50 value of 0.129 mg / kg. Compared with the commonly used nonoxynol, sanguinarine has better spermicidal indicators than nonoxynol, and it is expected to become a new type of high-efficiency external contraceptive for women.

The invention discloses six immune anti-fertility polypeptide segments, all of which have excellent immunogenicity. The six immune anti-fertility polypeptide segments can stimulate the organisms to generate specific antibodies and induce the anti-fertility function. According to the fertility comparison of antigen polypeptide immunized mice and the immunized mice in a control group (KLH (keyhole limpet homocyanin) control group), immunization by the six polypeptide antigens mixed group can significantly reduce the farrowing rate of the male and the female mice, wherein litter sizes in the male and the female mice is respectively reduced by 62.8% and 50%. The athletic ability and activity of sperms are significantly reduced in the HE6 polypeptide group or the mixed group immunized male mice. The polypeptide with high-efficiency anti-fertility effect establishes the foundation for research of the mouse-specific immune anti-fertility vaccines, and can be used for application of various anti-fertility preparations.

The invention relates to the field of medicines, and discloses an anti-fertility medicine, wherein the anti-fertility medicine contains a podophyllotoxin compound and is applied to spermicidal contraception for mammals. Also disclosed is a pharmaceutical preparation; the pharmaceutical preparation contains an active ingredient, wherein the active ingredient is the anti-fertility medicine provided by the invention. The invention also provides an application of the podophyllotoxin compound in preparation of the anti-fertility medicine, wherein the anti-fertility medicine is applied for spermicidal contraception in genital tracts of the mammals. The podophyllotoxin compound provided by the invention can be directly used as a drug for spermicidal contraception in genital tracts of female mammals, especially as a drug for spermicidal contraception in genital tracts of human female, and has high safety and low toxicity to human body organs. Therefore, the anti-fertility medicine provided by the invention has quite important practical significance and application promotion value for improving the level of reproductive health.

The invention relates to the technical field of preparation of chemical components of traditional Chinese medicines, in particular to a flavonoid compound and its preparation method and application. The structural formula of the compound is as follows: the present invention also provides the preparation method of the above-mentioned flavonoids, using the dry root of Rhynchosia volubilis Lour. Gel column chromatography and high performance liquid chromatography in the system. The flavonoids can competitively inhibit progesterone by acting on calcium ion channels of human sperm, and then affect sperm activity, showing anti-fertility effects, can be applied to the preparation of sperm function-related drugs and contraceptive drugs, and are derived from anti-fertility Traditional Chinese medicine with high safety and efficacy.

The invention discloses a follicle-stimulating hormone recipient B cell epitope and an antigenic peptide containing the epitope; the follicle-stimulating hormone recipient B cell epitope of the invention has amino acid sequences shown in SEQ ID No.1 and has single component and simple structure, and are free of the impact of bad epitopes in natural proteantigens, and immunoreactions initiated by the epitope of the invention has strong pertinence; the antigenic peptide containing the follicle-stimulating hormone recipient B cell epitope, is obtained by connecting a T accessory cell epitope with the follicle-stimulating hormone recipient B cell epitope in series while the T cell epitope has the amino acid sequences shown in SEQ I D No.2; the antigenic peptide can induce that an antibody of the follicle-stimulating hormone recipient B cell epitope is targeted with high titer in a specific manner and a reversible anti-fertility function with high efficiency and safety is produced, thereby being capable of preparing a male pregnancy vaccine, having significant social and economic benefits and having important value in theory and wide application prospect.

The invention mainly relates to a control method of grassland rodents. Brandt's vole is one of main rodents in Inner Mongolia Grassland. The invention provides a novel method for controlling the number of the Brandt's vole population by using the mint oil. According to the novel method, a mature non-overwintering Brandt's vole is used as an experimental subject, mint oil with different concentrations is used for performing intragastric administration on the Brandt's vole, autopsy is performed, the embryo implantation situation in the uteruse of the Brandt's vole is statistically recorded, and the uterus is collected for performing histopathological research and analysis. Experiments show that the mint oil has a significant anti-fertility effect on the Brandt's vole, and can effectively reduce the embryo implantation number of the Brandt's vole and reduce the reproduction rate of the Brandt's vole. Researches shown that the mint oil can effectively control the reproduction rate of the Brandt's vole, and thus control the number of the Brandt's vole population, so that sufficient evidence is provided for application of the mint oil as a potential botanical sterilant to rodent sterility control.

The invention relates to the field of medicines, and discloses an anti-fertility medicine, wherein the anti-fertility medicine contains a podophyllotoxin compound and is applied to spermicidal contraception for mammals. Also disclosed is a pharmaceutical preparation; the pharmaceutical preparation contains an active ingredient, wherein the active ingredient is the anti-fertility medicine provided by the invention. The invention also provides an application of the podophyllotoxin compound in preparation of the anti-fertility medicine, wherein the anti-fertility medicine is applied for spermicidal contraception in genital tracts of the mammals. The podophyllotoxin compound provided by the invention can be directly used as a drug for spermicidal contraception in genital tracts of female mammals, especially as a drug for spermicidal contraception in genital tracts of human female, and has high safety and low toxicity to human body organs. Therefore, the anti-fertility medicine provided by the invention has quite important practical significance and application promotion value for improving the level of reproductive health.