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B cell epitope of follicle-stimulating hormone receptor and antigen peptides containing thereof

A follicle stimulating hormone, B cell technology, applied in the direction of anti-receptor/cell surface antigen/cell surface determinant immunoglobulin, medical preparations containing active ingredients, peptides, etc., can solve the problem of unable to meet contraceptive standards, contraceptive effect Unsatisfactory and other problems, to achieve the effects of major social and economic benefits, simple structure and single composition

Inactive Publication Date: 2009-02-11
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the contraceptive effect using natural protein as antigen is not ideal, and the contraceptive rate is only about 70%, which cannot meet the clinical contraceptive standard, and cannot overcome the common problems brought about by using natural protein antigen, such as immune tolerance, etc.

Method used

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  • B cell epitope of follicle-stimulating hormone receptor and antigen peptides containing thereof
  • B cell epitope of follicle-stimulating hormone receptor and antigen peptides containing thereof
  • B cell epitope of follicle-stimulating hormone receptor and antigen peptides containing thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The antigenic peptide of this example is formed by linking the C-terminal of the T helper cell epitope and the N-terminal of the B-cell epitope of FSHR through a flexible linker -GGG-, and its amino acid sequence is LSEIKGVIVHRLEGVGGGIELRFVLTKLRVI.

[0030]The synthesis of the antigenic peptide in this example was completed on a solid-phase peptide synthesizer (ABI 431A type). Using the standard fluorenylmethoxycarbonyl (Fmoc) scheme, initially select 0.0125mmol of polystyrene (polystyrene, PS) resin (ABI Company, USA), and make the peptide chain from the C-terminus to the N-terminus one by one according to the amino acid sequence designed. Extension; the amount of various Fmoc amino acids is 0.1mmol; before each step of condensation, activate the carboxyl group of Fmoc amino acids with 1-hydroxybenzotriazole (HOBt) / N, N'-dicyclohexylcarbodiimide (Dcc), after condensation The Fmoc protecting group was removed with an N-methylpyrrolidone (NMP) solution containing 20% ​​b...

Embodiment 2

[0032] The antigenic peptide of this example is formed by linking the C-terminal of the B cell epitope of FSHR and the N-terminal of the T helper cell epitope through a flexible linker -GGS-, and its amino acid sequence is IELRFVLTKLRVIGGSLSEIKGVIVHRLEGV.

[0033] The synthesis of the antigenic peptide in this example was completed on a solid-phase peptide synthesizer (ABI 431A type). Using the standard Fmoc scheme, the synthesis method is the same as that described in Example 1.

Embodiment 3

[0035] The antigenic peptide of this example is formed by linking the C-terminal of the T helper cell epitope and the N-terminal of the B-cell epitope of FSHR through a flexible linker -KK-, and its amino acid sequence is LSEIKGVIVHRLEGVKKIELRFVLTKLRVI.

[0036] The synthesis of the antigenic peptide in this example was completed on a solid-phase peptide synthesizer (ABI 431A type). Using the standard Fmoc scheme, the synthesis method is the same as that described in Example 1.

[0037] Identification of antigenic peptides of the present invention

[0038] Methods: (1) Grouping of experimental animals: 80 clean-grade male Balb / c mice aged 6-8 weeks (provided by the Experimental Animal Center of Beijing Academy of Military Medical Sciences) with a body weight of 10-16 g were randomly divided into 4 groups: Invention of antigenic peptide), PBS control group (replacing antigenic peptide with phosphate buffer saline PBS), irrelevant peptide control group (replacing antigenic pe...

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Abstract

The invention discloses a follicle-stimulating hormone recipient B cell epitope and an antigenic peptide containing the epitope; the follicle-stimulating hormone recipient B cell epitope of the invention has amino acid sequences shown in SEQ ID No.1 and has single component and simple structure, and are free of the impact of bad epitopes in natural proteantigens, and immunoreactions initiated by the epitope of the invention has strong pertinence; the antigenic peptide containing the follicle-stimulating hormone recipient B cell epitope, is obtained by connecting a T accessory cell epitope with the follicle-stimulating hormone recipient B cell epitope in series while the T cell epitope has the amino acid sequences shown in SEQ I D No.2; the antigenic peptide can induce that an antibody of the follicle-stimulating hormone recipient B cell epitope is targeted with high titer in a specific manner and a reversible anti-fertility function with high efficiency and safety is produced, thereby being capable of preparing a male pregnancy vaccine, having significant social and economic benefits and having important value in theory and wide application prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a follicle stimulating hormone receptor B cell epitope and an antigenic peptide containing the epitope. Background technique [0002] Population explosion and unplanned pregnancy have been a worldwide public health problem. Although there are a variety of contraceptive methods, they all have their limitations. For example, male contraceptives can only use condoms or vasectomy. The former has a high failure rate, and the latter is irreversible and may produce unexpected severe immunity learning consequences. Therefore, it is urgent to develop a high-efficiency, safe, long-lasting, reversible and cheap contraceptive method. [0003] Follicle-stimulating hormone (FSH) is one of the most important reproductive hormones in the human body. Its regulation of male reproductive function is through the specific receptor expressed only on the surface of Sertoli cell membrane, that is, FSH rec...

Claims

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Application Information

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IPC IPC(8): C07K7/08C07K19/00C07K16/00C07K16/28A61K39/00A61P15/16
Inventor 李晋涛阎萍吴玉章梁志清陈正琼何畏
Owner ARMY MEDICAL UNIV
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