B cell epitope of epididymal protease inhibitor and multiple antigenic peptide containing thereof
A protease inhibition and B cell technology, applied in the direction of protease inhibitor immunoglobulin, immunoglobulin, peptide, etc. with anti-peptide structure, can solve the problems of unsatisfactory, contraceptive rate stay, high failure rate, etc., and achieve major social Benefits and economic benefits, reversible anti-fertility effects, highly targeted effects
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Embodiment 1
[0028] The multiple antigenic peptide (MAP-I-2) of the present embodiment is a two-branched peptide, such as figure 2 As shown in (A), the dendritic core is composed of a lysine, and the T helper cell epitope and the B cell epitope of Eppin are covalently linked to different branches of the dendritic core.
[0029] The multiple antigenic peptides of this example were synthesized on a solid-phase peptide synthesizer (ABI431A). Adopt the standard fluorenylmethoxycarbonyl (Fmoc) scheme, initially select 0.0125mmol of PSC resin (ABI company in the United States), and make the peptide chain extend from the C-terminus to the N-terminus one by one according to the designed amino acid sequence; the amount of various Fmoc amino acids is 0.1mmol; 1-hydroxybenzotriazole (HOBt) / N, N'-dicyclohexylcarbodiimide (Dcc) is used to activate the carboxyl group of the protected amino acid before each step of condensation, and the carboxyl group containing the volume percentage concentration of 20% ...
Embodiment 2
[0031] The multiple antigenic peptide (MAP-I-4) of the present embodiment is a four-branched peptide, such as figure 2 (B) As shown in (B), the dendritic core is polymerized by three lysines, and the T helper cell epitope and the B cell epitope of Eppin are respectively covalently linked to different branches of the dendritic core, and connected to the branch The molar ratio of B cell epitopes to T helper epitopes is 1:1.
[0032] The synthesis of multiple antigenic peptides in this example was completed on a solid-phase peptide synthesizer (ABI431A type). Using the standard Fmoc scheme, the synthesis method is the same as that described in Example 1.
Embodiment 3
[0034] The multiple antigenic peptide (MAP-I-8) of the present embodiment is an eight-branched peptide, such as figure 2 As shown in (C), the dendritic core is polymerized by seven lysines, and the T helper cell epitope and the B cell epitope of Eppin are respectively covalently linked to different branches of the dendritic core, and connected to the branch The molar ratio of B cell epitopes to T helper epitopes is 1:1.
[0035] The synthesis of multiple antigenic peptides in this example was completed on a solid-phase peptide synthesizer (ABI431A type). Using the standard Fmoc scheme, the synthesis method is the same as that described in Example 1.
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