Epitope of epididymal protease inhibitor B cell and antigen peptides containing thereof
A protease inhibition, B cell technology, applied in the direction of anti-peptide structure protease inhibitor immunoglobulin, immunoglobulin, peptide, etc. Benefits and economic benefits, reversible anti-fertility effects, highly targeted effects
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Embodiment 1
[0029] The antigenic peptide of this example is formed by linking the C-terminal of the T helper epitope and the N-terminal of the B-cell epitope of Eppin through a flexible linker -GGG-, and its amino acid sequence is LSEIKGVIVHRLEGVGGGCQGNNNNFQSKAN.
[0030]The synthesis of the antigenic peptide in this example was completed on a solid-phase peptide synthesizer (ABI431A type). Using the standard fluorenylmethoxycarbonyl (Fmoc) scheme, initially select 0.0125mmol of polystyrene (polystyrene, PS) resin (ABI Company, USA), and make the peptide chain from the C-terminus to the N-terminus one by one according to the amino acid sequence designed. Extension; the amount of various Fmoc amino acids is 0.1mmol; before each step of condensation, activate the carboxyl group of Fmoc amino acids with 1-hydroxybenzotriazole (HOBt) / N, N'-dicyclohexylcarbodiimide (Dcc), after condensation The Fmoc protecting group was removed with an N-methylpyrrolidone (NMP) solution containing 20% by vol...
Embodiment 2
[0032] The antigenic peptide of this example is formed by linking the C-terminal of the B cell epitope of Eppin and the N-terminal of the T helper cell epitope through a flexible linker -GGS-, and its amino acid sequence is CQGNNNNFQSKANGGSLSEIKGVIVHRLEGV.
[0033] The synthesis of the antigenic peptide in this example was completed on a solid-phase peptide synthesizer (ABI431A type). Using the standard Fmoc scheme, the synthesis method is the same as that described in Example 1.
Embodiment 3
[0035] The antigenic peptide of this example is formed by linking the C-terminal of the T helper epitope and the N-terminal of the B-cell epitope of Eppin through a flexible linker -KK-, and its amino acid sequence is LSEIKGVIVHRLEGVKKCQGNNNNFQSKAN.
[0036] The synthesis of the antigenic peptide in this example was completed on a solid-phase peptide synthesizer (ABI431A type). Using the standard Fmoc scheme, the synthesis method is the same as that described in Example 1.
[0037] Identification of antigenic peptides of the present invention
[0038] Methods: (1) Grouping of experimental animals: 40 clean-grade male Balb / c mice aged 6-8 weeks (provided by the Experimental Animal Center of Beijing Academy of Military Medical Sciences) with a body weight of 18-20 g were randomly divided into 4 groups: Invention of antigenic peptide), PBS control group (replacing antigenic peptide with phosphate buffer saline PBS), irrelevant peptide control group (replacing antigenic peptide...
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