B cell epitope of epididymal protease inhibitor and multiple antigenic peptide containing thereof

A protease inhibition and B cell technology, applied in the direction of protease inhibitor immunoglobulin, immunoglobulin, peptide, etc. with anti-peptide structure, can solve the problems of unsatisfactory, contraceptive rate stay, high failure rate, etc., and achieve major social Benefits and economic benefits, reversible anti-fertility effects, highly targeted effects

Inactive Publication Date: 2009-02-11
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At present, male contraception is still limited to the traditional use of condoms or vasectomy, the former has a high failure rate, and the latter is irreversible and may have unexpected and serious immunological consequences, such as persistently high levels of antisperm antibody drops degree and/or changes in testicular morphology, neither of which is satisfactory
Over the years, scientists at home and abroad have been

Method used

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  • B cell epitope of epididymal protease inhibitor and multiple antigenic peptide containing thereof
  • B cell epitope of epididymal protease inhibitor and multiple antigenic peptide containing thereof
  • B cell epitope of epididymal protease inhibitor and multiple antigenic peptide containing thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] The multiple antigenic peptide (MAP-I-2) of the present embodiment is a two-branched peptide, such as figure 2 As shown in (A), the dendritic core is composed of a lysine, and the T helper cell epitope and the B cell epitope of Eppin are covalently linked to different branches of the dendritic core.

[0029] The multiple antigenic peptides of this example were synthesized on a solid-phase peptide synthesizer (ABI 431A). Adopt the standard fluorenylmethoxycarbonyl (Fmoc) scheme, initially select 0.0125mmol of PSC resin (ABI company in the United States), and make the peptide chain extend from the C-terminus to the N-terminus one by one according to the designed amino acid sequence; the amount of various Fmoc amino acids is 0.1mmol; 1-hydroxybenzotriazole (HOBt) / N, N'-dicyclohexylcarbodiimide (Dcc) is used to activate the carboxyl group of the protected amino acid before each step of condensation, and the carboxyl group containing the volume percentage concentration of 20%...

Embodiment 2

[0031] The multiple antigenic peptide (MAP-I-4) of the present embodiment is a four-branched peptide, such as figure 2 (B) As shown in (B), the dendritic core is polymerized by three lysines, and the T helper cell epitope and the B cell epitope of Eppin are respectively covalently linked to different branches of the dendritic core, and connected to the branch The molar ratio of B cell epitopes to T helper epitopes is 1:1.

[0032] The synthesis of multiple antigenic peptides in this example was completed on a solid-phase peptide synthesizer (ABI 431A type). Using the standard Fmoc scheme, the synthesis method is the same as that described in Example 1.

Embodiment 3

[0034] The multiple antigenic peptide (MAP-I-8) of the present embodiment is an eight-branched peptide, such as figure 2 As shown in (C), the dendritic core is polymerized by seven lysines, and the T helper cell epitope and the B cell epitope of Eppin are respectively covalently linked to different branches of the dendritic core, and connected to the branch The molar ratio of B cell epitopes to T helper epitopes is 1:1.

[0035] The synthesis of multiple antigenic peptides in this example was completed on a solid-phase peptide synthesizer (ABI 431A type). Using the standard Fmoc scheme, the synthesis method is the same as that described in Example 1.

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Abstract

The invention discloses an epididymis protease inhibition protein B-cell epitope and a multiple antigenic peptide containing the epitope; the epididymis protease inhibition protein B-cell epitope of the invention has amino acid sequences shown in SEQ ID No.1 and has single component and simple structure, and is free of the impact of bad epitopes in natural proteantigens, and immunoreactions initiated by the epitope of the invention has strong pertinence; the multiple antigenic peptide containing the epididymis protease inhibition protein B-cell epitope, is obtained by connecting a T accessory cell epitope with the epididymis protease inhibition protein B-cell epitope on a branch of a tree-based core formed by a bifunctional unit or a plurality of bifunctional units; the multiple antigenic peptide can induce that an antibody of the epididymis protease inhibition protein B-cell epitope is targeted with high titer in a specific manner and a reversible anti-fertility function with high efficiency and safety is produced, thereby being capable of preparing a male pregnancy vaccine, having significant social and economic benefits and having important value in theory and wide application prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to epididymis protease inhibitory protein B cell epitope and multiple antigenic peptides containing the epitope. Background technique [0002] Family planning is the basic national policy of our country. In order to effectively control the excessive population growth, the development of efficient, safe and reversible contraceptive methods has always been an important topic of family planning research. At present, male contraception is still limited to the traditional use of condoms or vasectomy, the former has a high failure rate, and the latter is irreversible and may have unexpected and serious immunological consequences, such as persistently high levels of antisperm antibody drops Degree and / or changes in testicular morphology, both of which are unsatisfactory. Over the years, scientists at home and abroad have been working on the development of male contraceptive vaccines, mainly t...

Claims

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Application Information

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IPC IPC(8): C07K7/08C07K19/00C07K16/00C07K16/38A61K39/00A61P15/16
Inventor 李晋涛吴玉章陈正琼梁志清阎萍何畏
Owner ARMY MEDICAL UNIV
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