4 results about "Extracellular dna" patented technology

The invention relates to a modified RNA with a synthetic cap structure and a modified coding region encoding for DNAse1 and / or DNAse1L3. The invention further relates to a saRNA and / or a circular RNA, and to a vector encoding the modified RNA. The invention also relates to a pharmaceutical composition for the use of treating a disease associated with the formation of extracellular DNA, including extracellular traps released from leukocytes, such as NETs.

This invention discloses a green control method and application for banana wilt disease based on banana extracellular DNA, belonging to the field of plant disease biological control technology. The method includes: extracting total DNA from healthy banana tissue, obtaining esDNA fragments of 100-500 bp size through physical shearing; preparing the esDNA into a working solution, and applying it to banana plants via root irrigation. This invention utilizes banana-derived esDNA as an immune elicitor, which not only directly inhibits the mycelial growth of *Fusarium oxysporum* var. *cube* (especially Foc TR4), but also systematically activates the immune response of banana plants, enhances the activity of their defensive enzymes, and reduces the accumulation of reactive oxygen species, thereby significantly reducing rhizosphere colonization of the pathogen, alleviating yellowing and wilting symptoms, and improving plant survival rate and biomass. This method is environmentally friendly, highly safe, and easy to operate, providing a new green solution for the sustainable management of banana wilt disease.

This invention belongs to the field of solid waste resource utilization technology, and relates to a method for synergistic reduction of antibiotic resistance genes in anaerobic digester residues through hydrothermal-induced iron-calcium-magnesium-silicon-aluminum mineralization. Anaerobic digester residues are used as the treatment target. After screening, homogenization, and solids content adjustment, a composite mineralization inducer is added and the pH is adjusted, and a mineralization reaction is carried out under closed hydrothermal conditions. During the reaction, the hydrothermal effect promotes the rupture of resistant bacterial cells, the deconstruction of extracellular polymers, and DNA strand breaks in the digester residues. Simultaneously, iron, calcium, magnesium, silicon, and aluminum components undergo in-situ mineralization with phosphates, carbonates, humic substances, and dissolved organic matter in the digester residues, forming a composite mineral phase. This complexes, precipitates, encapsulates, and passivates antibiotic resistance gene fragments, mobile genetic elements, extracellular DNA, antibiotic residues, and heavy metals. This method achieves synergistic reduction of resistance genes, decrease in migration risk, weakening of selection pressure, and resource utilization of digester residues, and has advantages such as short treatment cycle, high reduction efficiency, good product stability, and strong engineering applicability.

A method is provided for enriching extracellular DNA from a biological sample comprising extracellular DNA and extracellular vesicles, wherein the method comprises: (a) preparing a binding mixture comprising—the biological sample, —a solid phase comprising anion exchange groups, —an acidic binding buffer comprising a buffering agent, and binding extracellular DNA to the solid phase comprising anion exchange groups; (b) separating the solid phase with the bound extracellular DNA from the remaining binding mixture, wherein the remaining binding mixture comprises extracellular vesicles. The method may furthermore comprise processing the remaining binding mixture to enrich one or more biological targets of interest therefrom, wherein processing may comprise (c) enriching as biological targets extracellular vesicles and / or extracellular RNA from the remaining binding mixture.