10 results about "Extracellular dna" patented technology

The invention relates to a modified RNA with a synthetic cap structure and a modified coding region encoding for DNAse1 and / or DNAse1L3. The invention further relates to a saRNA and / or a circular RNA, and to a vector encoding the modified RNA. The invention also relates to a pharmaceutical composition for the use of treating a disease associated with the formation of extracellular DNA, including extracellular traps released from leukocytes, such as NETs.

The invention belongs to the technical field of environmental functional materials and water pollution control, discloses a cerium monatomic nano-enzyme as well as a preparation method and application thereof, and effectively solves the problem that an existing advanced oxidation technology lacks specificity on ARGs degradation and the problems that a traditional nano-enzyme is non-selective in catalytic mechanism and undefined in active site structure. According to the cerium monatomic nano-enzyme, a metal organic framework UiO-67-N is used as a carrier, pore channels of the carrier are modified through a nitrogen-containing ligand 2, 2 '-bipyridine-5, 5'-dicarboxylic acid, anchoring sites of cerium monatomic are constructed, a monatomic active center with a Ce-N6 coordination structure is formed, and cerium stably exists in a material framework in an atomic-scale dispersion form. According to the method, efficient recognition and cutting of extracellular DNA phosphodiester bonds in a neutral water environment are achieved, ARGs structures are directly destroyed, horizontal transfer pathways of the ARGs structures are blocked, and an enzyme simulation catalysis tool is provided for precise blocking and controlling of the ARGs in a water body.

This invention discloses a green control method and application for banana wilt disease based on banana extracellular DNA, belonging to the field of plant disease biological control technology. The method includes: extracting total DNA from healthy banana tissue, obtaining esDNA fragments of 100-500 bp size through physical shearing; preparing the esDNA into a working solution, and applying it to banana plants via root irrigation. This invention utilizes banana-derived esDNA as an immune elicitor, which not only directly inhibits the mycelial growth of *Fusarium oxysporum* var. *cube* (especially Foc TR4), but also systematically activates the immune response of banana plants, enhances the activity of their defensive enzymes, and reduces the accumulation of reactive oxygen species, thereby significantly reducing rhizosphere colonization of the pathogen, alleviating yellowing and wilting symptoms, and improving plant survival rate and biomass. This method is environmentally friendly, highly safe, and easy to operate, providing a new green solution for the sustainable management of banana wilt disease.

The application relates to a polypeptide functionalized gold nanocluster targeting neutrophils, a preparation method thereof and application thereof in preparation of an anti-thrombus drug, and belongs to the field of biomedical nanomaterials. The nanocluster is formed by Au-S coordination self-assembly of a thiol-containing targeting polypeptide and gold atoms. The polypeptide can specifically recognize neutrophil elastase (NE), realizes targeting of activated neutrophils and inhibits enzyme activity of the neutrophils. The nanocluster inhibits formation of neutrophil extracellular traps (NETs) induced by phorbol ester (PMA) or activated platelets, and shows reduction of histone citrullination (CitH3) level, extracellular DNA release and active oxygen (ROS) generation. In-vivo experiments show that the gold nanocluster is enriched at a thrombus site, and significantly reduces thrombus load in a deep vein thrombosis model and an FeCl3-induced arterial thrombosis model. The application plays an anti-thrombus role by inhibiting NETs.

The invention provides a multifunctional polyrotaxane hydrogel FDS. NO loaded on the FDS hydrogel has the capability of promoting cell migration, proliferation and angiogenesis at the same time. The FDS hydrogel can inhibit the activation of a downstream pathway by adsorbing extracellular DNA (cfDNA), thereby reducing the release of inflammatory factors. Animal experiments further prove that the FDS hydrogel not only can sterilize and promote cell proliferation and reconstruction of a diabetic wound area, but also can promote angiogenesis of a diabetic rat wound. Therefore, the FDS hydrogel has remarkable advantages in diabetes wound management.

The application discloses a method for removing extracellular DNA / antibiotic resistance genes in red soil, and belongs to the field of environmental pollution treatment. The application mainly solves the problem of extracellular DNA / antibiotic resistance gene pollution in red soil. The application is based on an extracellular DNA (eDNA) remover, a chemical reagent, which is used for degrading the extracellular DNA in the red soil, and then removing antibiotic resistance genes (eARGs) contained in the extracellular DNA, so as to help people correctly evaluate the risk of resistance gene transmission.

This invention belongs to the field of solid waste resource utilization technology, and relates to a method for synergistic reduction of antibiotic resistance genes in anaerobic digester residues through hydrothermal-induced iron-calcium-magnesium-silicon-aluminum mineralization. Anaerobic digester residues are used as the treatment target. After screening, homogenization, and solids content adjustment, a composite mineralization inducer is added and the pH is adjusted, and a mineralization reaction is carried out under closed hydrothermal conditions. During the reaction, the hydrothermal effect promotes the rupture of resistant bacterial cells, the deconstruction of extracellular polymers, and DNA strand breaks in the digester residues. Simultaneously, iron, calcium, magnesium, silicon, and aluminum components undergo in-situ mineralization with phosphates, carbonates, humic substances, and dissolved organic matter in the digester residues, forming a composite mineral phase. This complexes, precipitates, encapsulates, and passivates antibiotic resistance gene fragments, mobile genetic elements, extracellular DNA, antibiotic residues, and heavy metals. This method achieves synergistic reduction of resistance genes, decrease in migration risk, weakening of selection pressure, and resource utilization of digester residues, and has advantages such as short treatment cycle, high reduction efficiency, good product stability, and strong engineering applicability.

A method is provided for enriching extracellular DNA from a biological sample comprising extracellular DNA and extracellular vesicles, wherein the method comprises: (a) preparing a binding mixture comprising—the biological sample, —a solid phase comprising anion exchange groups, —an acidic binding buffer comprising a buffering agent, and binding extracellular DNA to the solid phase comprising anion exchange groups; (b) separating the solid phase with the bound extracellular DNA from the remaining binding mixture, wherein the remaining binding mixture comprises extracellular vesicles. The method may furthermore comprise processing the remaining binding mixture to enrich one or more biological targets of interest therefrom, wherein processing may comprise (c) enriching as biological targets extracellular vesicles and / or extracellular RNA from the remaining binding mixture.