15 results about "DNase activity" patented technology

Provided herein are methods and compositions comprising AcrIIIA1, the first Type III-A specific anti-CRISPR protein. AcrIIIA1 can bind to the Cas10 effector complex and can attenuate its DNase activity and second messenger production. Additionally, AcrIIIA1 associates with fragmented t(m)RNAs (acrRNAs) that undergo extensive degradation during phage infection. AcrIIIA1 can directly antagonize core Cas10 machinery while acrRNAs absorb collateral damage dealt by accessory nucleases. These compositions and methods can, inter alia, provide robust protection against Type III CRISPR-Cas immunity.

The use of one or more polypeptides having DNase activity and one or more detersive polymers during laundry for reducing redeposition of malodor on a textile, for removing malodor from a textile and / or for maintaining or improving the whiteness of a textile is described. Furthermore, laundry detergent compositions comprising, as a DNase component, one or more polypeptides having DNase activity having at least 80% sequence identity to SEQ ID NO: 1, 2, 3, 4 or 5 and one or more terephthalic acid-based decontamination polymers as a decontamination polymer component are disclosed.

Provided herein are methods and compositions comprising AcrIIIA1, the first Type III-A specific anti-CRISPR protein. AcrIIIA1 can bind to the Cas10 effector complex and can attenuate its DNase activity and second messenger production. Additionally, AcrIIIA1 associates with fragmented t(m)RNAs (acrRNAs) that undergo extensive degradation during phage infection. AcrIIIA1 can directly antagonize core Cas10 machinery while acrRNAs absorb collateral damage dealt by accessory nucleases. These compositions and methods can, inter alia, provide robust protection against Type III CRISPR-Cas immunity.

The present disclosure provides endonuclease variants having improved properties, such as hyperactivity and / or low indiscriminate single strand DNase activity, relative to the corresponding wild-type endonucleases.

The invention relates to the technical field of parasite detection, in particular to a kit and a method for detecting toxoplasma gondii based on RPA-CRISPR / Cas12a. The kit comprises a crRNA sequence, wherein the crRNA sequence is as shown in SEQ ID NO.7, SEQ ID NO.11, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.21, SEQ ID NO.23 or SEQ ID NO.30, and the crRNA sequence is as shown in SEQ ID NO.7, SEQ ID NO.11, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.21, SEQ ID Or the difference between the nucleotide sequence and the nucleotide sequence is not more than 2 basic groups. The kit further comprises an RPA primer pair, Cas12a protein and ssDNA, Cas12a carries out DNase activity, the single-stranded DNA marked with fluorophore is cut, and whether a sample to be detected contains toxoplasma gondii DNA or not can be judged by detecting a fluorescence signal. When being used for detecting toxoplasma gondii, the system has the characteristics of short reaction time, strong specificity, high sensitivity, small instrument dependence, simplicity in operation and the like, the effect is outstanding, and the sensitivity is consistent with that of fluorescent quantitative PCR.

The invention relates to a non-guanidine salt type compatible nucleic acid extraction virus preservation solution as well as a preparation method and application thereof, and the virus preservation solution comprises P (HEMA-BAMPD hydrochloride), a buffer agent, a chelating agent, a saccharide stabilizer and an osmotic pressure regulator. The P (HEMA-BAMPD hydrochloride) is used for replacing guanidine salt and small molecule quaternary ammonium salt, the inactivation rate effect on enveloped RNA / DNA viruses such as SARS-CoV-2 viruses and influenza viruses is good, no irritation is generated, and the method is friendly to operators and the environment; the macromolecular structure realizes multi-point adsorption and has strong organic matter interference resistance, and a small amount of blood, sputum and other components in the sample do not influence the inactivation effect. Moreover, the P (HEMA-BAMPD hydrochloride), the chelating agent and the saccharide stabilizer have a synergistic effect, so that the activities of RNase and DNase can be effectively inhibited.

Provided is a method for cleaning a medical device comprising contacting the device with a wash liquor comprising two or more enzymes selected from the group consisting of a protease, an enzyme having DNase activity and an enzyme having hexosaminidase activity.

Provided is a method of cleaning a medical device comprising contacting the device with a cleaning solution comprising two or more enzymes selected from the group consisting of a protease, an enzyme having DNase activity, and an enzyme having hexosaminidase activity.

The present invention relates to a polypeptide having alkaline phosphatase activity, a cleaning composition comprising a polypeptide having alkaline phosphatase activity and optionally a polypeptide having DNase activity and the use of the composition in a cleaning process such as laundry.

The invention discloses a preparation method of a spherical DNA nano machine for detecting O6-methylguanine-DNA methyltransferase (MGMT) and application of the spherical DNA nano machine in detection of the expression level of tumor cells MGMT. The method comprises the following steps: firstly, designing a DNA walker with MGMT recognition and signal amplification functions, and fixing the DNA walker on the surface of a gold nanosphere through sulfydryl modification to construct a spherical DNA nanomachine; when the MGMT interacts with a DNA Walker chain, a methylated basic group G on DNA enzyme can be repaired, so that the activity of the DNA enzyme is recovered, an enzyme digestion reaction is initiated, a Track chain marked with a fluorophore is broken and released, a fluorescence signal change is generated, and high-sensitivity detection of the MGMT is realized. The method can effectively detect the MGMT protein in a low concentration range, and has a good biomedical application prospect.

The present application relates to a kind of non-guanidine salt type compatible nucleic acid extraction virus preservative and preparation method and application, the virus preservative, including P (HEMA-BAMPD) hydrochloride, buffer, chelating agent, saccharide stabilizer and osmotic pressure regulator.The present application replaces guanidine salt and small molecule quaternary ammonium salt with P (HEMA-BAMPD) hydrochloride, and the inactivation rate effect of SARS-CoV-2 virus, influenza virus and other envelope type RNA / DNA viruses is good, and there is no irritability, and it is friendly to operating personnel and environment;Polymer structure realizes multiple point adsorption, and the ability of resistance to organic interference is strong, and a small amount of blood, sputum and other components in sample do not affect inactivation effect.Moreover, P (HEMA-BAMPD) hydrochloride, chelating agent and saccharide stabilizer synergistic effect can effectively inhibit RNase, DNase activity.

The present disclosure provides endonuclease variants with improved properties, such as higher activity and / or lower promiscuous single-stranded DNase activity, as compared to the corresponding wild-type endonuclease.

The invention provides a probe, a freeze-drying reagent, a lateral flow chromatography test strip, a kit and a method for detecting DNase activity, and relates to the technical field of biology. Based on an antigen-antibody reaction and an immunochromatography technology, a DNA probe of which the 5'end is labeled with 6-carboxyl fluorescein and the 3 'end is labeled with biotin is adopted as a substrate, and when a sample to be detected contains DNase and is mixed with the substrate, the DNase can specifically recognize and cut a phosphodiester bond on the DNA probe, so that DNA fragmentation is caused. The test strip is inserted into the reacted reagent, when a sample does not contain DNase, the DNA probe is not subjected to enzyme digestion and is fixed on the quality control line to be combined with streptavidin, a red strip appears on the quality control line, and a test strip detection line does not develop color; when DNase exists in a sample, the DNA probe is subjected to enzyme digestion and is fixed on the detection line to be combined with the second antibody, and a red strip appears on the detection line.

The invention discloses a method for rapidly and accurately detecting group B streptococcus (GBS) based on CRISPR / Cas12a, when nucleic acid of the GBS forms a ternary complex with Cas12a and crRNA, a RuvC structural domain of the Cas12a in the complex shows DNase activity, a labeled single-stranded DNA probe can be cut, and the Cas12a can be used for rapidly and accurately detecting the group B streptococcus. The detection is further performed by adopting methods such as fluorescence detection, lateral flow chromatography detection or lateral flow chromatography detection matched with an intelligent mobile terminal and the like. The method disclosed by the invention can efficiently, accurately and quickly detect GBS, has the advantages of simplicity in operation, adaptability to various detection scenes and the like, and has important significance on early monitoring and diagnosis of GBS and antibiotic treatment decision.

RNA-targeting Cas12a2 complex allows for rationale design of Cas12a2 into a versatile enzyme capable of non-specifically degrading distinct types of nucleic acid targets depending on mutations of the active site residues and residues that stabilize bound targets. These mutations allow for tuning of output signal associated with RNA detection. By mutating specific residues, indiscriminate single-stranded RNase and DNase and double-stranded DNase activity can be modified to only cleave single-stranded DNA and single-stranded RNA, or only single-stranded DNA. This allows for diagnostic tools which can provide a detection. Residues involved in binding the non-self vs. self-recognition signal (PFS) can also be modified so larger subsets of nucleic acid targets can be recognized.