Application of honokiol in inhibiting staphylococcus aureus biofilm

A technology of staphylococcus and honokiol, applied in the field of biomedicine, can solve the problems of repeated attacks and serious infections that cannot be cured for a long time

Inactive Publication Date: 2014-12-03
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that many serious infections cannot be cured and relaps

Method used

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  • Application of honokiol in inhibiting staphylococcus aureus biofilm
  • Application of honokiol in inhibiting staphylococcus aureus biofilm
  • Application of honokiol in inhibiting staphylococcus aureus biofilm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Microdilution method for drug sensitivity test: Dilute the stock solution of honokiol with MHB medium, and add 100 μL per well to a 96-well plate. Dilute the bacterial solution from the overnight culture with MHB medium to an OD600 of 0.4 (equivalent to 5 × 10 8 CFU / mL) and further diluted to a concentration of 10 6 CFU / mL. 100 μL of bacterial suspension was added to each well, MHB was used as negative control, and bacterial suspension without drug was used as positive control. After mixing evenly, it was placed in a 37 °C incubator for static culture, and the results were observed after 24 h. The lowest concentration that can inhibit bacterial growth is the MIC value of the strain. The bacteria in each well are pipetted evenly and then poured into the MHA plate. The lowest concentration that does not grow bacteria is the MBC value. The experiment was repeated 3 times, and the median was taken as the final value. and honokiol against Staphylococcus aureus suspensio...

Embodiment 2

[0022] Drug susceptibility test by plate wiping method: single colonies from solid TSB culture plates were put into test tubes containing 5 mL of TSB, and placed in a shaker at 37 °C for overnight culture at 180 r / min; the bacterial solution was cultured with TSB (containing 0.5% glucose) Base diluted to an OD600 of 0.4 (equivalent to 5 × 10 8 CFU / mL) and further diluted to a concentration of 10 5 CFU / mL; carefully add 200 μL of diluted bacterial solution to each well of a 96-well plate, and place it in a 37 °C incubator for 24 h. After the biofilm was formed, the suspension was discarded, and 250 μL of PBS was added to each well to gently wash off the free bacteria. The drug was serially diluted twice in TSB medium (0.0625-512 μg / mL) and added to each well. Incubate at 37°C for 24 h. The suspension was discarded, washed once with PBS, and 250 μL of PBS was added. After blowing the biofilm evenly, 10 μL of each well was spread on TSA, and incubated at 37 °C for 24 h. The ...

Embodiment 3

[0025] Disc diffusion method experiment: use sterile cotton swabs to spread the bacteria on the plate medium, sterilize the tweezers with an alcohol lamp flame and stop for a while, and take the drug sensitive tablets and stick them on the surface of the plate medium regularly. The plate medium was placed in a 37°C incubator for 24 hours, and the effect was observed. When the drug concentration increased, the inhibition zone also increased significantly (see figure 2 ).

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Abstract

The invention discloses an application of honokiol in preparing medicines for inhibiting staphylococcus biofilms. Drug susceptibility test is utilized to detect the inhibiting effect of honokiol respectively on floating bacteria of staphylococcus aureus and biofilms, and the inhibiting effect of honokiol is further verified by drawing a killing curve and adopting a crystal violet semiquantitative method. Furthermore, the influence on polysaccharide intercellular adhesion (PIA) synthesis and extracellular DNA (eDNA) release and investigations on the transcriptional level of coding fibronectin-binding protein genes fnbA and fnbB indicate that the honokiol has an excellent effect for inhibiting growth of staphylococcus aureus biofilms.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the application of honokiol for inhibiting staphylococcus aureus biofilm. Background technique [0002] Infection caused by bacteria has become one of the main causes of infectious diseases, which is characterized by drug resistance and refractory, and can also resist the immune clearance of the body, causing great harm to the health of humans and animals, and also to the Other aspects of people's lives have increasingly serious impacts, which have attracted great attention in basic and clinical research. Bacterial biofilm refers to a structured bacterial community composed of bacterial cells attached to the surface of an inert or active entity and a hydrated matrix enclosing the bacteria. Bacterial biofilms widely exist on various surfaces containing water and humidity, including water pipes, industrial pipes, ventilation equipment, medical equipment, and even human tissu...

Claims

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Application Information

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IPC IPC(8): A61K31/05A61P31/04
Inventor 郭娜李文莉孟日增于录刘宗慧赵星辰史册
Owner JILIN UNIV
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