Method and equipment for improving the quality of mammalian spermatozoa
A semen, sperm cell technology, applied in the field of assisted reproduction in mammals, can solve problems such as lack of information
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[0042] To corroborate the aforementioned evidence and the actual effects of light therapy on different descriptive parameters of improving sperm cell motility-viability and tolerance of sperm cells to environmental stress (which is considered at 37°C for a period of more than 1 hour) size, in different laboratory studies using pig semen as a mammalian model.
[0043] Studies were performed with porcine sperm because they are readily available material and because they are a good model for extrapolation of results to other mammals, including humans.
[0044] The study was carried out using samples from 15 healthy adult male pigs aged 2-3 years and from 5 different breeds (Pietrain, Duroc, Large White, Belgian Landrace and English Landrace). These samples were obtained by manual stimulation and immediately diluted in commercial diluent to a final sperm concentration of 3 × 10 7 spermatozoa / ml in a final volume of 90 mL and stored at 16-17°C.
[0045] Deposit two aliquots of 1....
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