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Protein capable of adjusting sperm capacitation and use thereof

A technology for sperm capacitation and use, applied in the biological field, to solve problems such as poorly understood functions

Inactive Publication Date: 2009-04-01
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although it is speculated that HongrES1 may be a factor related to mammalian reproduction, the function of HongrES1 protein is still poorly understood in the prior art

Method used

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  • Protein capable of adjusting sperm capacitation and use thereof
  • Protein capable of adjusting sperm capacitation and use thereof
  • Protein capable of adjusting sperm capacitation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0068] As an embodiment of the present invention, administering an antagonist of HongrES1 (such as an antibody or small interfering RNA) to a male animal or implanting it in a female animal (such as the reproductive tract) can inhibit the fertility of a mammal (such as contraception) role.

[0069] As a preferred mode of the present invention, a HongrES1 antagonist is provided, and the antagonist is a small interfering RNA that can specifically interfere with the transcription of the HongrES1 gene. The small interfering RNA has a sequence shown in SEQ ID NO: 5 or SEQ ID NO: 7; or a sequence complementary to SEQ ID NO: 5 or SEQ ID NO: 7, the above-mentioned small interfering RNA is obtained through repeated screening, and has Good inhibitory activity of HongrES1 transcription.

[0070] Methods of making and using siRNA are well known to those skilled in the art. The siRNA can be prepared in the form of double-stranded nucleic acid, which contains a sense strand and an antisen...

Embodiment 1

[0166] Localization of HongrES1 in the epididymis on the protein level in Example 1

[0167] Using conventional antibody preparation techniques, the inventors obtained highly sensitive and highly specific HongrES1 antibodies. The hybridization test was carried out using the antibody, and the HongrES1 protein was used as the antigen. The result is as figure 1 A, when different concentrations of antigens are combined with the antibody, the bands obtained by hybridization are also different in depth. The higher the concentration of antigen, the darker the hybridization bands. It can be seen that the antibodies prepared by the present invention have high sensitivity and high specificity.

[0168] The epididymal tissues were subjected to Western blot test to detect the expression of HongrES1 in the tissues. The results showed that HongrES1 was expressed in the caudal epididymis but not in the head, body and testis of the epididymis, as shown in figure 1 b.

[0169] The expressi...

Embodiment 2

[0174]Example 2 Developmental changes and regulation of HongrES1 expression

[0175] Using the animal model of whole body castration and androgen supplementation in rats, the expression changes of HongrES1 were detected after castration and androgen supplementation.

[0176] The results showed that the androgen of the rats decreased to zero on the first day after castration, and with this decrease, the mRNA expression of HongrES1 also decreased, and it was close to zero on the seventh day after the operation. After supplementation with androgen (testosterone) on the seventh day after surgery, androgen rose rapidly, and the mRNA expression of HongrES1 also showed a similar increase pattern. It suggested that the expression of HongrES1 mRNA was regulated by androgen, as shown in Figure 2A-B.

[0177] The developmental changes of the mRNA and protein levels of HongrES1 were tracked and determined. The results showed that the expression of HongrES1 could be detected 30 days after...

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Abstract

The invention belongs to the biotechnology field and discloses a use of an epididymis specific expression gene HongrES1 or an agonist thereof or an antagonist in the preparation of compositions for regulating sperm capacitation of mammals; the invention further discloses a method for screening effective substances for regulating the sperm capacitation of the mammals by taking the epididymis specific expression gene as a target point. The invention firstly reveals that the HongrES1 has an important role on the aspect of regulating the sperm capacitation and the fertility of the mammals, thereby providing the effective target point for researching male reproduction.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically, the invention relates to an epididymis-specific expression gene and its function. Background technique [0002] The spermatozoa produced in the testis are basically mature in terms of their morphological structure and chromatin, but they do not yet have the ability to motility, sperm-egg recognition and combination. They must enter the adjacent tissue of the testis - the epididymis to mature. During the migration of sperm in the epididymis, a series of changes are also taking place in various parts of the sperm, such as the migration of sperm cytoplasm droplets, further changes in the morphology of sperm such as cytoplasm and acrosome; the condensation of sperm nuclear chromatin, Increased resistance to nucleases; significant changes in sperm membrane charges, sugar groups, lipids, and proteins; abnormal sperm are gradually swallowed, absorbed, or degraded. Sperm undergo a se...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K39/395A61K9/02A61K9/107A61K9/20A61K9/48A61P15/08C12Q1/68
Inventor 张永莲周玉传郑敏石其贤胡志红刘强陈文颖
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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