Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Animal sex control method based on Rbmy gene editing and application

A technology of animal and sex, applied in the field of genetic engineering, can solve problems such as male sterility, sperm deformity, gene copy number reduction, etc.

Active Publication Date: 2016-08-17
SOUTH CHINA AGRI UNIV
View PDF2 Cites 35 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of these genes are only expressed in the germline of male mice and are closely related to spermatogenesis. Knocking out part of the long arm of the Y chromosome will reduce the copy number of this part of the gene, resulting in sperm deformities and even male sterility, while increasing the number of females. Birth ratio of offspring

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Animal sex control method based on Rbmy gene editing and application
  • Animal sex control method based on Rbmy gene editing and application
  • Animal sex control method based on Rbmy gene editing and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0038] The present invention will be described in further detail below in conjunction with the accompanying drawings.

[0039] pU6-sgRNA empty vector, pT7-sgRNA empty vector, pT7-Cas9 empty vector, phCas9 vector and pGCS empty vector were obtained from the Guangzhou Center for Medicine and Health, Chinese Academy of Sciences; pEGFP empty vector gene sequence was obtained from GenBank accession number: U55762.1, the The carrier was purchased from Bao Biological Engineering (Dalian) Co., Ltd.

[0040] 1. Application of CRISPR / Cas9 system to cut mouse Y chromosome target site selection

[0041] According to the prediction of the online software (https: / / chopchop.rc.fas.harvard.edu / index.php) combined with the recognition characteristics of sgRNA and the least off-target probability, this experiment selected 6 targets of the Y chromosome multi-copy gene Rbmy The specific information of the target site is shown in Table 1 below.

[0042]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an animal sex control method based on Rbmy gene editing and application. The method controls animal sex by using CRISPR / Cas9 system to specifically cut Rbmy gene on Y chromosome. Rbmy gene encodes a germ cell specific nucleoprotein, this protein is an important factor related to spermatogenesis, and by cutting Rbmy gene, it is possible to deactivate Rbmy gene, thus Y sperm or fertilized ovum with Y sperm is deactivated and cannot develop into a normal embryo, birth ratio of female animals is finally increased and sex control is achieved. Therefore, sex ratio of animal offspring can be controlled through the method so that the ratio of female animals with better economic value is increased and production efficiency and economic effect are greatly improved.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a method and application for realizing sex control of animals based on editing the Rbmy gene. Background technique [0002] Sex control refers to a technology that artificially intervenes in the reproductive process of animals so that female animals can produce offspring that meet a specific sex. Animal sex control technology is of great significance in preventing human genetic diseases, improving livestock production capacity, animal genetic breeding and protection of endangered and rare animals. [0003] At present, there are more than 300 kinds of sex-linked genetic diseases related to the sex of the baby at birth (such as hemophilia, red-green color blindness and hereditary nephritis, etc.), the use of sex control technology in early pregnancy can predict the occurrence of sex-linked genetic diseases, So as to achieve the purpose of prenatal diagnosis and prevent...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/85C12N15/877
CPCC07K14/47C12N15/8509C12N15/877C12N2800/107C12N2800/80C12N2810/10
Inventor 吴珍芳李紫聪杨晓峰李崇刘德武蔡更元
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com