Method for producing parasitic wasps of fruit flies by means of drosophila melanogaster

Abstract

The invention provides a method for mass-producing parasitic wasps of fruit flies by means of drosophila melanogaster. By fast and simply collecting pupae of the drosophila melanogaster, mass breedingand parasitism of the parasitic wasps of the fruit flies are simply completed, in a pupal stage, the parasitic wasps of the fruit flies are fast and simply separated from the unparasitized drosophilamelanogaster, and the number and a sex ratio of the parasitic wasps of the fruit flies are fast and simply regulated and controlled, so that full mating and a proper parasitism rate are ensured.

Description

technical field [0001] The invention relates to the field of biological control of agricultural pests, and aims to provide a method for mass-producing Drosophila parasitoids by using Drosophila melanogaster. Background technique [0002] The use of natural enemy insects for population control is a common means of pest biological control. At present, a variety of natural enemy insects such as predatory mites, Trichogrammas, and ladybugs have been successfully commercialized for field pest control. The common feature of these natural enemy insects is that they can carry out large-scale factory production, and use relatively stable insect states such as eggs and pupae for packaging, transportation and field release. Drosophila (including Drosophila melanogaster Drosophila melanogaster Drosophila imi D.immigrans Drosophila spotted wing D .suzukii etc.) are important agricultural pests that harm a variety of fruits and vegetables. Several species of parasitic wasps have b...

AI Technical Summary

Problems solved by technology

In the collection of host fruit fly pupae, CN201710749534, CN201911101413, and CN201610213523 need to manually collect clean fruit fly pupae with brushes, tweezers, water spray, filter screens, etc., while CN201710749534 needs to manually remove the parent fruit flies in the container, Therefore, it is time-consuming and labor-intensive, and does not meet the needs of mass production.

Moreover, the hosts used in all these schemes are fruit flies that are good at flying. They are pests themselves and are easy to escape. After escaping, a series of problems such as cross-contamination in the factory building and harm to fruits and vegetables in the field will occur.

In the breeding and parasitic links of Drosophila spp., CN201910246022, CN201710749534, and CN201610213523 need to manually collect and introduce parasitoids, which is time-consuming and laborious

CN201920411820 and CN201520807526 raise the host and the bee together in the same space, so it is impossible to control the age of the pupae, and it is also difficult to separate the parasitized pupae from the larvae in the medium, and because the mature larvae stage has been eaten and consumed by the larvae The culture medium is relatively sticky, and the parasitoids are easy to drown in the medium

In the separation process of Drosophila spp. and non-parasitic hosts, CN201520738270 and CN201520807526 use the volume difference to separate the insects by using a blocking net. The obtained parasitoids are adults and need to be re-collected before they can be used for packaging and transportation. CN201920411820 utilizes the first eclosion of the host fruit flies properties and phototaxis, but the step of using phototaxis separation is slow, and the separation is likely to be incomplete, which is not suitable for the needs of mass production

Finally, the technical solution for the maintenance of the sex ratio of Drosophila chinensis is still blank, and it is almost purely relying on manual sorting, which greatly depends on the work proficiency of the sorter, and is not suitable for mass production.

However, if the sex ratio control is not carried out, it will lead to incomplete mating, fewer female bees to emerge, and can not play the role of parasitic prevention and control.

In summary, the common disadvantage of the existing technical solutions for the production of Drosophila chinensis is that it needs to consume a lot of labor and time to carry out fine operations in each link. If the scale is enlarged, the time and labor required will also be doubled. Expansion, and there is the possibility of host escape and harm

There is currently no protocol suitable for the large-scale production of Drosophila

In addition, technical solutions for mass reproduction of larval parasitoids of Drosophila and other pupal parasitoids are still blank

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