15 results about "Drosophila buzzatii" patented technology

The present application relates to the technical field of insect screening and collecting, in particular to a virgin fly collecting device and method for fruit fly genetic research; the device comprises a guide pipe and a camera assembly and the like structure, two elastic bags are arranged on the guide pipe, and a preset interval is arranged between the two elastic bags as a detection section; the camera assembly is arranged opposite to the detection section, and is used for shooting photos of fruit flies located in the detection section; real-time image acquisition of fruit flies passing through the detection section is realized through the camera assembly; virgin flies and non-virgin flies are automatically distinguished by combining a deep learning visual recognition program built in an upper computer; and the airflow direction is controlled by an electromagnetic three-way valve to drive the elastic bag and the air blowing pipe to complete the shunting of fruit flies; the whole process does not need manual anesthesia, magnifying glass identification and the like operation, the labor cost is significantly reduced, and the problems of long screening time and large labor intensity of virgin flies in the prior art are solved.

This invention discloses an experimental apparatus for anesthetizing fruit flies, belonging to the field of instrumentation. The apparatus includes a transparent box with an internal partition dividing the interior into a purification chamber and an operating chamber. The partition has several absorption holes and a removable hole-covering plate. Embedded gloves are located on the left and right sides of the box. A removable absorption box is located at the top of the purification chamber. The operating chamber has a fly inlet tube and an anesthesia tube at the top, and a fly outlet tube at the bottom. The fly inlet tube also contains a culture bottle inlet mechanism. This experimental apparatus can create an ether environment for anesthetizing fruit flies, keeping them in a low-anesthesia state and preventing them from waking up and flying away during the fly selection / sorting process. The absorption box absorbs the ether after the experiment, preventing direct release into the atmosphere and avoiding direct inhalation of toxic gases by the experimenter, thus protecting the physical and mental health of the experimenter.

The application discloses a fruit fly feeding behavior analysis method and device, and relates to the technical field of insect behavior analysis, and comprises the following steps: setting different environment parameter groups, performing a fruit fly feeding monitoring test, collecting the motion video of a single fruit fly at a feeding device, and dividing the video frames into continuous characteristic segments. By analyzing the characteristic segments, the starting point and the ending point of each round of suspected feeding process are determined, and the food surface images before and after the suspected feeding are obtained. The structural feature changes of the surface images before and after the suspected feeding are compared to determine whether the feeding behavior occurs, if the feeding behavior occurs, the round of process is defined as the feeding process, and the feeding amount is represented based on the surface feature changes. The feeding times, the average feeding interval and the average feeding amount of the fruit fly under different environment parameters are analyzed, and the influence of the environment parameters on the fruit fly feeding behavior is output. The scheme has significant advantages in time resolution and result accuracy, and can especially analyze the short-time feeding behavior of the fruit fly.

The application belongs to the technical field of pest control, and discloses application of a transcription factor CG17328 in control of drosophila melanogaster. The application discloses application of CG17328 in regulation of insect reproductive capacity. The application firstly screens a transcription factor for regulating the 'dumping' process of nurse cells in oogenesis of drosophila melanogaster by using transcriptome sequencing, genetics and molecular biology and other related technologies and means. The transcription factor is a ZAD-C2H2 type zinc finger protein CG17328. CG17328 is mainly located in the nuclei of nurse cells and oocytes in the late stage of oogenesis. CG17328 can affect the oogenesis process by regulating the 'dumping' process of nurse cells. The loss of CG17328 expression leads to significant decrease of the number of mature oocytes generated by ovaries and the hatching rate of eggs, which indicates that the loss or inhibition of CG17328 expression can achieve the control effect on drosophila melanogaster.

The application discloses a model for evaluating microtoxicity of evodia extract based on fruit fly behavior and a construction method and application thereof, and belongs to the technical field of toxicity evaluation. In order to accurately, quickly and efficiently evaluate the microtoxicity of the evodia extract, avoid the fluctuation of the corresponding indexes of each behavior experiment of the fruit fly and better reflect the olfactory and gustatory behaviors of the fruit fly, the application successively connects a bidirectional selection feeding experiment, a Y type experiment and a proboscis extension experiment to construct a model for evaluating the microtoxicity of the evodia extract based on the fruit fly behavior, and the model has the characteristics of high accuracy, quickness and high efficiency and can be used for evaluating whether the evodia extract solution reaches a lethal concentration.

The invention provides a blood-sucking midge efficient repellent and a screening and identifying method thereof, and relates to the technical field of biology. According to the invention, an odor receptor gene FtaiOr1 which is highly expressed at the head of a female gnat is obtained through identification, and a transgenic drosophila melanogaster strain Or22ab- / -GAL4 which can be used for screening a blood-sucking midge repellent compound is constructed through heterologous expression of the gene in a drosophila melanogaster ab3A neuron; and UAS-FtaiOr1 (UAS-FtaiOr1). Six blood sucking midge repelling compounds (camphor, eudesmol, (+)-anisone, alpha-terpilenol, (S)-cis-verbenol and isoterpinene) are obtained by screening of the fruit fly strain. The compounds can be used independently or in a compounded manner, are friendly to non-target organisms and environment, can be directly applied to repelling and on-site prevention and control of blood sucking midges, and have important application value in the aspects of reducing midge bites and reducing the propagation risk of entomophilous diseases.

The utility model provides a kind of convenient egg taking device of real fly, comprising: attractant containing cup and oviposition needle insertion cup, attractant containing cup and oviposition needle insertion cup are connected by thread, the oviposition needle insertion cup is installed in the upper of attractant containing cup, the oviposition needle insertion cup surface is evenly provided with needle hole with 0.1-1mm diameter. The device is simple in structure, reasonable in design, easy to assemble and use, can quickly and conveniently collect the egg particles of Bactrocera dorsalis and Bactrocera cucurbitae, and the size of oviposition hole limits the oviposition of fruit fly, avoids the phenomenon that different kinds of fly larvae will appear when collecting larvae in rotten melon and rotten fruit in field, and the application scenario is wide.

The present invention relates to insect neuropeptide compounds of the AKH family and analogs thereof having activity against insects, such as hemiptera, diptera, lepidoptera, cockroaches and / or coleopteran insects, such as aphids and fruit flies, and their use as insect control agents (e.g. Insecticides) and plant protection agents.

The utility model discloses a kind of fruit fly traps, belong to fruit fly trapping field, including trapping box, trapping box side is equipped with several trapping holes of annular arrangement, trapping box top is detachably connected with suspension mechanism, suspension mechanism includes top cover, top cover is threadedly connected with trapping box top, top cover is fixedly connected with installation shell, lifting mechanism is set in installation shell, lifting mechanism includes electric push rod. Advantageous effect lies in: suspension mechanism, lifting mechanism and collection mechanism are set, by the water-absorbing cotton set on installation rod and the storage tube installed on connecting frame, water-absorbing cotton can automatically supplement liquid trapping agent, improve the degree of automation, save the work of staff frequently supplementing liquid trapping agent, convenient to use, and using scraper and briquetting can clean the inner wall of trapping box when supplementing liquid trapping agent, and kill fruit fly, improve trapping effect, and top cover and bottom shell adopt detachable design, easy to maintain and clean.

The present invention relates to bifunctional insect neuropeptide compounds and analogs thereof having activity against insects, such as hemiptera, diptera, lepidoptera, cockroaches and / or coleopteran insects, such as aphids and fruit flies, and their use as insect control agents (e.g. Insecticides) and plant protection agents.

1. Name of the product in this design: Fly Killer (Mushroom Shape). 2. Purpose of this design: For the capture and killing of pests such as fruit flies, flies, and small flying insects. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

This invention discloses a method for effectively inhibiting the reproduction of fruit fly larvae in the nutrient soil during Arabidopsis thaliana cultivation, relating to the field of soil science. The method includes: ① planting Arabidopsis thaliana seedlings; ② preparation of a non-absorbent material; ③ application of the non-absorbent material; ④ watering in a tray. This invention has the following advantages and positive effects: By preparing a non-absorbent material, this method maintains the continuous dryness of the surface nutrient substrate, effectively inhibiting the reproduction of fruit flies on the surface of the nutrient soil during Arabidopsis thaliana cultivation. This prevents Arabidopsis thaliana seedlings from being eaten by fruit fly larvae, or even the entire plant from being eaten and dying. It also effectively inhibits the outbreak of surface terrestrial fungi, helping researchers using Arabidopsis thaliana as experimental material to efficiently and labor-savingly cultivate plant materials suitable for measuring experimental data in greenhouses.

The present invention relates to natural or natural-like analogues of insect adipokinetic hormone (AKH) neuropeptide [Pyr]-LTFTSSWGG-[NH2] (SEQ ID NO:2) having activity against insects, for example hemipteran, dipteran, lepidopteran and / or coleopteran insects, such as aphids, moths and fruit flies, and their use as insect control agents (e.g. insecticides) and plant protection agents.

The invention discloses a function and an application of virus source tyrosine phosphatase in development of male testis of plutella xylostella, namely an application of a protein tyrosine phosphatase gene CvBV22-9 coded by cotesia vestalis virus in induction of apoptosis of testis cells of larvae of plutella xylostella. According to the invention, the CvBV22-9 gene is screened and identified as a main factor for inducing testis apoptosis, and the gene is continuously and highly expressed in a parasitized testis tissue and has tissue specificity. After the expression of the CvBV22-9 is knocked down, the testis apoptosis rate is obviously reduced; and about 90.80% of testis cell apoptosis can be induced by using recombinant baculovirus to over-express the gene. The low-concentration recombinant baculovirus can also destroy the testis structure and lead to decrease of the egg laying amount of mating female insects. According to the invention, a transgenic fruit fly model is successfully constructed, specific expression of CvBV22-9 in fruit fly testis is realized by using a UAS-Gal4 system, and 97.30% of testis apoptosis is induced.