The invention discloses a construction method and application of a human TFEB
gene transgenic fruit fly model, and the construction method comprises the following steps: S1, inserting a human TFEB
gene coding region sequence into a UASp-HA-attB vector, and constructing a UASp-TFEB-HA-attB
plasmid; s2, introducing the
plasmid constructed in the step S1 into a
drosophila melanogaster embryo with attP; and S3, hybridizing the hatched G0-generation
drosophila melanogaster with the white-eye
drosophila melanogaster, and screening out the red-eye drosophila melanogaster, namely the UASp-TFEB-HA transgenic drosophila melanogaster. According to the invention, a coding sequence of a human TFEB
gene is introduced into fruit flies by using a
transgenic technology to prepare UASp-TFEB-HA transgenic fruit flies; the expression of the TFEB is driven in different tissues, cells can be induced to have phenotypes such as
autophagy level increase similar to that of overexpressed TFEB in mammalian cells, it is indicated that the TFEB has physiological functions in drosophila melanogaster and mammals, a good model can be provided for analyzing a tumorigenesis mechanism caused by TFEB activation, and the TFEB can be used for preparing a tumor
cell model. And a tool is provided for
medicine screening of diseases caused by abnormal TFEB activity.