10 results about "Gene drive" patented technology

The invention discloses a targeted nutrient source mining method based on beef cattle intestinal type-host gene interaction, particularly relates to the field of biological information data processing, and is used for solving the problems that existing nutrient source development lacks molecular mechanism verification and is poor in targeting. The method comprises the following steps: firstly, dividing nutrition-associated intestinal types based on microbiome and transcriptome data, and constructing a co-expression network to screen host co-expression specific genes driven by the specific intestinal types; then, establishing a flora metabolite ligand set and host protein receptor model, executing molecular conformation search and Gibbs free energy calculation, and screening high-activity targeting effect factors based on physical affinity; and finally, traversal matching and quantitative screening are carried out on the natural raw material liquid chromatography-mass spectrometry data by using the targeted effect factors, and a targeted nutrition source recommendation list aiming at the specific intestine type is generated. According to the method, multi-omics correlation analysis and molecular thermodynamics verification are fused, and an accurate development closed loop from micromechanism analysis to macroscopic raw material matching is constructed.

This invention discloses a soybean bidirectional promoter, a recombinant vector, its construction method, and its applications, belonging to the field of plant genetic engineering technology. The soybean bidirectional promoter of this invention can simultaneously drive gene expression in two directions. By tandemly connecting three genes at the front and back ends of this bidirectional promoter, it is possible to achieve simultaneous expression of six genes driven by a single promoter. Furthermore, by utilizing this bidirectional promoter in combination with the ZQ6 enhancement sequence and the 35S:MdMYB10 expression cassette, a method for simultaneously and significantly enhancing the expression levels of more than six genes was developed, and based on this, a method for enabling plants to emit noticeable self-luminescence under dark conditions was developed. The method for efficient and coordinated multi-gene transformation mediated by the soybean bidirectional promoter provided by this invention can be applied to constructing a soybean multi-gene coordinated transformation system, achieving rapid aggregation of superior traits, and cultivating new soybean germplasm with excellent traits.

Described herein are embodiments relating to manipulation of populations and sex ratio in populations through DNA sequence modifications.

The present disclosure relates compositions, systems, and methods for treating, inhibiting, decreasing, reducing, ameliorating, and / or preventing a cancer or a proliferative disease using a selection gene drive therapy.

The invention discloses a tandem expression recombinant industrial strain for efficiently producing epsilon-polylysine and application of the tandem expression recombinant industrial strain, the strain TG1003-ddcpgkakpls is obtained by integrating a tandem expression cassette on a genome attB site of a wild strain TG1003 of streptomyces albidus, the strain TG1003-ddcpgkakpls is preserved in China Center for Type Culture Collection on June 16, 2025, and the preservation number of the strain TG1003-ddcpgkakpls is CCTCC M 20251400; the tandem expression cassette sequentially comprises a pls gene driven by a kasOP promoter, and a ddc gene, a pgk gene and an ak gene which are respectively driven by a permEP promoter. Related genes produced by epsilon-PL are expressed in series on a genome of a wild type strain, and different promoters are combined with the genes, so that the stable expression quantity of the related genes is improved, and the yield of epsilon-PL is further improved.

The invention discloses a cotton GhMYB36b promoter for regulating and controlling formation of a Kjeldahl band and application of the cotton GhMYB36b promoter in cadmium stress tolerance. The GhMYB36b promoter disclosed by the invention is high in specificity, high in regulation and control efficiency, safe in source and good in adaptability. The GhMYB36b promoter obviously activates downstream gene expression only under a cadmium stress condition, almost has no basic expression under a non-stress condition, can realize cadmium stress specific regulation and control of a target gene, and avoids burden on cotton growth caused by continuous expression of the gene. Under cadmium stress, the expression quantity and enzyme activity of the GUS gene driven by the promoter are obviously higher than those of no-load control, and the regulation efficiency of the promoter is obviously superior to that of a conventional constitutive promoter. The promoter is a cotton endogenous promoter, when the promoter is applied to plants such as cotton, the risks of gene silencing and the like caused by an exogenous promoter can be reduced, the adaptability is higher, and a high-quality element is provided for cadmium-tolerant gene engineering improvement of the cotton and other plants.

A method includes accessing a digital pathology image that depicts tumor cells sampled from a subject. A plurality of patches may be selected from the digital pathology image, wherein each of the patches depicts tumor cells. A mutation prediction may be generated for each of the patches, wherein the mutation prediction represents a prediction of a likelihood that an actionable mutation appears in the patch. Based on the plurality of mutation predictions, a prognostic prediction related to one or more treatment regimens for the subject may be generated. The prognostic prediction may be based on determining one or more mutational contexts of the digital pathology image as an unknown driver or a tumor suppressor, an oncogene driver mutation, or a gene fusion.

The invention belongs to the technical field of bioengineering, and provides a tobacco vascular tissue specific expression promoter pNtERD6L and application thereof, the nucleotide fragment of the promoter pNtERD6L is cloned, a pNtERD6L + GUS expression vector is constructed, agrobacterium tumefaciens-mediated tobacco genetic transformation is carried out, the effect of the promoter pNtERD6L in tobacco plants is evaluated, and the application of the promoter pNtERD6L in the tobacco plants is promoted. Results show that the promoter pNtERD6L can drive a target gene to be specifically expressed in the tobacco vascular tissue, so that adverse effects caused by continuous and efficient expression of an exogenous gene driven by a constitutive promoter in other tissues of the tobacco are avoided.

The application discloses a targeted nutrient source mining method based on beef cattle intestinal type-host gene interaction, and particularly relates to the field of biological information data processing, and is used for solving the problems of lack of molecular mechanism verification and poor targeting of existing nutrient source development. First, based on the microbiome and transcriptome data, the nutrient-related intestinal type is divided, and the co-expression network is constructed to screen the host co-expression specific genes driven by the specific intestinal type. Then, the model of the group metabolite ligand set and the host protein receptor is established, the molecular conformation search and the Gibbs free energy calculation are performed, and the high-activity targeted effect factor is screened based on the physical affinity. Finally, the targeted effect factor is used for traversing matching and quantitative screening of natural raw material liquid chromatography-mass spectrometry data, and a targeted nutrient source recommendation list for the specific intestinal type is generated. The application combines multi-omics correlation analysis and molecular thermodynamic verification, and constructs a precise development closed loop from micro mechanism analysis to macro raw material matching.

According to implementations of the present disclosure, a regimen for cell analysis is provided. According to the scheme, firstly, gene information related to genes included in a source cell and a target cell is determined; secondly, based on the gene information, feature representations corresponding to the source cell and the target cell are determined. Then, a driver gene is determined based on the feature representation, the driver gene being used to convert the source cell into the target cell by gene expression regulation. Thereby, a feature representation of the source cell and the target cell can be determined based on the gene information, and a driver gene can be determined based on the feature representation. In this way, prediction of driver genes can be achieved.