35 results about "Gene drive" patented technology

The invention discloses a tomato LACS2 gene promoter and application thereof, and belongs to the technical field of biology. On one hand, the invention provides the tomato LACS2 gene promoter, and on the other hand, the invention provides application of the tomato LACS2 gene promoter in promoting target gene expression in plants. According to the invention, a tomato LACS2 gene promoter fragment (788bp) is constructed to drive an EGFP-GUS (Enhanced Green Fluorescent Protein-Glucuronidase) gene fusion expression vector. GUS histochemical staining and laser confocal microscopy observation prove that the promoter fragment can guide normal expression of the reporter gene in the tomato epidermis. Therefore, the LACS2 promoter can be used for researching development, quality improvement and postharvest preservation of tomato peels and specific expression of exogenous genes in the tomato peels.

The invention discloses a watermelon transgenic screening expression cassette and application thereof, and belongs to the technical field of biology. According to the invention, an expression cassette of a TagRFP gene driven by a d35s promoter is constructed on a pCambia1300 vector, and a recombinant vector is obtained. A recombinant vector is introduced into watermelon cells by utilizing an agrobacterium-mediated genetic transformation technology, and a red fluorescent protein TagRFP gene is integrated into a watermelon genome, so that transgenic watermelons can be screened and identified by taking red fluorescence as an identifier in the whole growth period of watermelon genetic transformation. Compared with a traditional screening method, the screening method has the advantages that the screening process is greatly simplified, and the transgenic watermelon and the non-transgenic watermelon are clearly distinguished, so that the genetic transformation efficiency of the watermelon is remarkably improved.

The invention belongs to the technical field of strain metabolism modification, and particularly relates to a streptomyces avermitilis strong promoter and application. The nucleotide sequence of the streptomyces avermitilis strong promoter provided by the invention is as shown in SEQ ID NO. 4. The strong promoter can express and regulate a key gene, and the mRNA relative expression level of the gene driven by the strong promoter can still be kept stable in the 48-96-hour growth stage of the streptomyces avermitilis, which indicates that the strong promoter can keep high activity in the 48-96-hour growth stage of the streptomyces avermitilis, so that the target gene can be stably and highly expressed. Therefore, the strong promoter can be used for constructing recombinant plasmids for expressing key genes for synthesizing target natural products and streptomyces avermitilis recombinant bacteria, and the constructed streptomyces avermitilis recombinant bacteria can efficiently produce the target natural products.

The invention relates to the field of plant breeding, and particularly provides a method for fixing rice heterosis by using an OsZFPP gene. The method comprises the following steps: firstly, constructing an OsZFPP1 gene or OsZFPP2 gene expression cassette driven by an OsECA1 promoter; then, an expression box of rice with three target points of OsPAIR1, OsREC8 and OsOSD1 being knocked out of CRISPR / Cas9 is constructed; integrating the expression cassette into the same vector to transform hybrid rice; screening three-gene homozygous mutation and OsZFPP positive plants, and identifying diploid cloned offspring through flow cytometry and genome sequencing. According to the method, the rice OsZFPP gene is combined with MiMe to provide a novel method for fixing the heterosis of the rice through apomixis, the heterosis of the rice can be successfully fixed through the method, an apomixis system with the high maturing rate can be obtained, and a novel solution is provided for fixing the heterosis of the rice through the apomixis.

The invention relates to the technical field of plant genetic engineering, in particular to application of a transcription factor OsMPS in increasing the content of rice 2-acetyl-1-pyrroline. The amino acid sequence of the transcription factor OsMPS is as shown in SEQ ID NO. 27. According to the invention, through overexpression of the OsMPS gene driven by the constitutive promoter in the rice, the content of 2-acetyl-1-pyrroline in the rice is obviously increased; and the 2-acetyl-1-pyrroline is not detected in the mutant of the knockout line. The method has the advantages that the OsMPS is used as a positive regulation factor to promote the synthesis of the rice 2-acetyl-1-pyrroline, and plays an important role in improving the rice fragrance quality.

The invention discloses a soybean bidirectional promoter, a recombinant vector and a construction method and application thereof, and belongs to the technical field of plant genetic engineering. The soybean bidirectional promoter can simultaneously drive gene expression in two directions, the front end and the rear end of the bidirectional promoter are respectively connected with three genes in series, and simultaneous expression of six genes can be driven by one promoter. The bidirectional promoter is combined with a ZQ6 enhancement sequence and a 35S: MdMYB10 expression cassette, a method for simultaneously and remarkably improving the expression level of more than six genes is developed, and a method for enabling a plant to emit obvious self-luminescence under a dark condition is developed based on the method. The soybean two-way promoter mediated multi-gene efficient synergistic transformation method provided by the invention can be applied to the aspects of constructing a soybean multi-gene synergistic transformation system, realizing rapid polymerization of excellent characters, cultivating new soybean germplasm with excellent characters and the like.

The invention discloses a targeted nutrient source mining method based on beef cattle intestinal type-host gene interaction, particularly relates to the field of biological information data processing, and is used for solving the problems that existing nutrient source development lacks molecular mechanism verification and is poor in targeting. The method comprises the following steps: firstly, dividing nutrition-associated intestinal types based on microbiome and transcriptome data, and constructing a co-expression network to screen host co-expression specific genes driven by the specific intestinal types; then, establishing a flora metabolite ligand set and host protein receptor model, executing molecular conformation search and Gibbs free energy calculation, and screening high-activity targeting effect factors based on physical affinity; and finally, traversal matching and quantitative screening are carried out on the natural raw material liquid chromatography-mass spectrometry data by using the targeted effect factors, and a targeted nutrition source recommendation list aiming at the specific intestine type is generated. According to the method, multi-omics correlation analysis and molecular thermodynamics verification are fused, and an accurate development closed loop from micromechanism analysis to macroscopic raw material matching is constructed.

Described herein are embodiments relating to manipulation of the composition, numbers and sex ratio in plant populations through DNA sequence modifications.

In various embodiments methods of utilizing gene drive constructs in asexual organisms such as viruses are provided. In certain embodiments the methods involve transfecting or infecting a cell with a modified DNA virus containing a gene drive construct; and infecting the cell with the target virus where the genome of said target DNA virus is modified by insertion of the gene drive construct into the genome of the target DNA virus and a population of modified target viruses (containing the gene drive construct) is produced.

This invention discloses a soybean bidirectional promoter, a recombinant vector, its construction method, and its applications, belonging to the field of plant genetic engineering technology. The soybean bidirectional promoter of this invention can simultaneously drive gene expression in two directions. By tandemly connecting three genes at the front and back ends of this bidirectional promoter, it is possible to achieve simultaneous expression of six genes driven by a single promoter. Furthermore, by utilizing this bidirectional promoter in combination with the ZQ6 enhancement sequence and the 35S:MdMYB10 expression cassette, a method for simultaneously and significantly enhancing the expression levels of more than six genes was developed, and based on this, a method for enabling plants to emit noticeable self-luminescence under dark conditions was developed. The method for efficient and coordinated multi-gene transformation mediated by the soybean bidirectional promoter provided by this invention can be applied to constructing a soybean multi-gene coordinated transformation system, achieving rapid aggregation of superior traits, and cultivating new soybean germplasm with excellent traits.

Described herein are embodiments relating to manipulation of the composition, numbers and sex ratio in plant populations through DNA sequence modifications.

Described herein are embodiments relating to manipulation of populations and sex ratio in populations through DNA sequence modifications.

The invention relates to the field of biotechnology engineering, in particular to recombinant escherichia coli based on cyanamide-induced flagellum assembly genes as well as a construction method and application of the recombinant escherichia coli. The recombinant escherichia coli based on the cyanamide-induced flagellum assembly gene is constructed by overexpressing the flagellum assembly gene driven by a cyanamide-induced promoter into escherichia coli 045. The recombinant Escherichia coli can strengthen the formation of a biological membrane, and the formation of the biological membrane is strengthened by adding cyanamide to precisely regulate and control the expression of a flagellum assembly gene in the process of preparing L-valine through fermentation. When the recombinant escherichia coli is used for preparing the L-valine through fermentation, the yield of the L-valine is increased, the fermentation efficiency is improved, and the fermentation period is shortened. The method has the advantages that the method is simple and convenient to operate, the cell can be recycled in the immobilized fermentation of the L-valine, the cost is saved, the continuity of the whole fermentation is enhanced, and a cell immobilization strategy based on a biological membrane is provided for the engineering application of an L-valine production system.

The invention discloses a construction method and application of a bacterial sensor for sensing iron and magnesium ions. The invention belongs to the field of microorganisms, and particularly relates to a construction method and application of a bacterial sensor for sensing iron and magnesium ions. The recombinant escherichia coli contains a super-folded green fluorescent protein sfGFP coding gene, a sensing protein PmrB mutant coding gene, a response regulatory protein PmrA mutant coding gene, a signal transduction protein PmrD coding gene and a strong ribosome binding site RBS. The gene contains a T7 RNA polymerase coding gene driven by a promoter of phosphorylase PmrG activated by regulatory protein PmrA and an sfGFP coding gene driven by a T7 promoter, and meanwhile, overexpresses a phosphoethanolamine transferase EptA coding gene. The bacterial sensor for detecting the concentration of iron ions is constructed, modification of lipopolysaccharide of the bacterial sensor can be changed through induction of iron and magnesium ions, and drug resistance of bacteria is enhanced.

The present invention relates to a method of generating a plurality of engineered subpopulations each comprising a gene drive.

The present disclosure relates compositions, systems, and methods for treating, inhibiting, decreasing, reducing, ameliorating, and / or preventing a cancer or a proliferative disease using a selection gene drive therapy.

The invention discloses a tandem expression recombinant industrial strain for efficiently producing epsilon-polylysine and application of the tandem expression recombinant industrial strain, the strain TG1003-ddcpgkakpls is obtained by integrating a tandem expression cassette on a genome attB site of a wild strain TG1003 of streptomyces albidus, the strain TG1003-ddcpgkakpls is preserved in China Center for Type Culture Collection on June 16, 2025, and the preservation number of the strain TG1003-ddcpgkakpls is CCTCC M 20251400; the tandem expression cassette sequentially comprises a pls gene driven by a kasOP promoter, and a ddc gene, a pgk gene and an ak gene which are respectively driven by a permEP promoter. Related genes produced by epsilon-PL are expressed in series on a genome of a wild type strain, and different promoters are combined with the genes, so that the stable expression quantity of the related genes is improved, and the yield of epsilon-PL is further improved.

The present invention relates to the treatment of cancer. In this study, the inventors focused on evaluating the efficiency of SIS3 in cancer treatment concurrently with identifying genes driving resistance to SMAD3 inhibition, aiming to find efficient and long-lasting treatment options. Novel high-throughput methods such as CRISPR-screen and long-read RNA sequencing were used to identify and precisely characterise the genes and signalling pathways driving resistance to SMAD3 inhibition. They identified a cell surface protein SLC16A1 / MCT1 as a driver of resistance to SIS3. They demonstrated that loss of MCT1 by CRISPR-Cas9 KO or inhibition by a specific inhibitor AZD3965 results in higher sensitivity to SMAD3 inhibition. To study if the effect is common to other similar cancer types, they also overexpressed MCT1 in KRAS-mutated lung adenocarcinoma cells and observed higher resistance to the inhibitory effect of SIS3. Synergistic inhibition of SMAD3 and MCT1 resulted in efficient melanoma cell death in a dose-dependent manner, suggesting the use of combined inhibition to be a beneficial treatment option for cancer. Thus, the present invention relates to a combination of a SLC16A1 inhibitor and a SMAD3 inhibitor for use in the treatment of a cancer in a subject in need thereof.

The invention discloses a cotton GhMYB36b promoter for regulating and controlling formation of a Kjeldahl band and application of the cotton GhMYB36b promoter in cadmium stress tolerance. The GhMYB36b promoter disclosed by the invention is high in specificity, high in regulation and control efficiency, safe in source and good in adaptability. The GhMYB36b promoter obviously activates downstream gene expression only under a cadmium stress condition, almost has no basic expression under a non-stress condition, can realize cadmium stress specific regulation and control of a target gene, and avoids burden on cotton growth caused by continuous expression of the gene. Under cadmium stress, the expression quantity and enzyme activity of the GUS gene driven by the promoter are obviously higher than those of no-load control, and the regulation efficiency of the promoter is obviously superior to that of a conventional constitutive promoter. The promoter is a cotton endogenous promoter, when the promoter is applied to plants such as cotton, the risks of gene silencing and the like caused by an exogenous promoter can be reduced, the adaptability is higher, and a high-quality element is provided for cadmium-tolerant gene engineering improvement of the cotton and other plants.

The present invention discloses a method and system for monitoring soybean cyst nematode population dynamics based on the Internet of Things (IoT). The method comprises collecting pest detection data and monitoring data within a preset area for gene-driven pest control, preprocessing the pest detection data and monitoring data, performing target screening on the pest detection data to obtain target detection data, and performing control risk assessment on the target detection data based on the monitoring data to obtain a control ratio, performing causal analysis on the pest detection data based on gene drive to obtain an ecological risk, constructing an optimized growth degree function based on the control ratio and the ecological risk, constructing an IoT-based soybean cyst nematode population dynamics monitoring model based on the optimized growth degree function, inputting the data to be monitored into the IoT-based soybean cyst nematode population dynamics monitoring model, and outputting monitoring results. This method improves the accuracy of nematode population dynamics monitoring and can be directly applied to nematode population dynamics monitoring systems.

The present disclosure relates compositions, systems, and methods for treating, inhibiting, decreasing, reducing, ameliorating, and / or preventing a cancer or a proliferative disease using a selection gene drive therapy.

The invention relates to the technical field of plant genetic engineering, and discloses application of a transcription factor OsMYB51 in increasing the content of volatile metabolites in rice, and the amino acid sequence of the transcription factor OsMYB51 is shown as SEQ ID NO.27. The invention further discloses a preparation method of the transcription factor OsMYB51. According to the invention, through overexpression of the OsMYB51 gene driven by a constitutive promoter in rice, the content of volatile metabolites of rice, especially 2-acetyl-1-pyrroline, is significantly increased. The method has the advantages that OsMYB51 is used as a positive regulatory factor to promote synthesis of rice 2-acetyl-1-pyrroline, and the method can be applied to rice fragrance quality improvement.

The invention discloses a specific expression promoter derived from upland cotton pollen and application of the specific expression promoter, and belongs to the technical field of plant promoters. The sequence of the pollen specific promoter is shown as SEQ ID NO.1, and a plant recombinant expression vector and recombinant bacteria of a GUS gene driven by a promoter fragment are constructed on the basis of the pollen specific promoter and are used for promoting pollen specific expression of a target gene in a plant. Experimental results show that the promoter can drive the GUS gene to be specifically expressed in the arabidopsis pollen, so that the pollen specific promoter has important significance in the fields of functional analysis and identification of plant anther growth and development related genes, establishment of plant male sterile lines and restoring lines and the like.

The present invention relates to the technical field of plant genetic engineering, and discloses the application of the transcription factor OsMYB51 in increasing the content of volatile metabolites in rice. The amino acid sequence of the transcription factor OsMYB51 is shown in SEQ ID NO. 27. By overexpressing the OsMYB51 gene driven by a constitutive promoter in rice, the present invention significantly increases the content of volatile metabolites in rice, especially 2-acetyl-1-pyrroline. The advantages are that OsMYB51 promotes the synthesis of 2-acetyl-1-pyrroline in rice as a positive regulator, and can be applied to the improvement of the fragrance quality of rice.

This invention discloses a method for constructing a bacterial sensor that senses iron and magnesium ions and its application. This invention belongs to the field of microbiology, specifically relating to a method for constructing a bacterial sensor that senses iron and magnesium ions and its application. Recombinant *Escherichia coli* contains a gene encoding a superfolded green fluorescent protein (sfGFP), a mutant gene encoding a sensor protein (PmrB), a mutant gene encoding a response regulatory protein (PmrA), a gene encoding a signal transduction protein (PmrD), and a strong ribosome binding site (RBS). It also contains a T7 RNA polymerase gene driven by the promoter of the phosphorylase PmrG activated by the regulatory protein PmrA, and a T7 promoter-driven sfGFP gene, while simultaneously overexpressing the gene encoding the phosphorylethanolamine transferase (EptA). This invention constructs a bacterial sensor for detecting iron ion concentration, and the modification of the lipopolysaccharide in the bacterial sensor can be altered by the induction of iron and magnesium ions, thereby enhancing bacterial drug resistance.

The application provides a grape VvKCS11 The application provides a promoter and application thereof, and belongs to the technical field of plant genetic engineering. ProKCS11 The nucleotide sequence is shown as SEQ ID NO. 1; a recombinant vector, an expression cassette, a recombinant cell or a recombinant bacteria containing the nucleotide sequence shown as SEQ ID NO. 1. ProKCS11 The application further provides a promoter with the function of responding to salt stress, drought stress, osmotic stress, ion stress or abscisic acid. ProKCS11 The salt stress, drought stress and ion toxicity can induce high expression of a downstream gene driven by the promoter, and the expression has certain tissue specificity. ProKCS11 The application further provides a transgenic plant. VvKCS11 The application can improve the stress resistance of the transgenic plant.

A method includes accessing a digital pathology image that depicts tumor cells sampled from a subject. A plurality of patches may be selected from the digital pathology image, wherein each of the patches depicts tumor cells. A mutation prediction may be generated for each of the patches, wherein the mutation prediction represents a prediction of a likelihood that an actionable mutation appears in the patch. Based on the plurality of mutation predictions, a prognostic prediction related to one or more treatment regimens for the subject may be generated. The prognostic prediction may be based on determining one or more mutational contexts of the digital pathology image as an unknown driver or a tumor suppressor, an oncogene driver mutation, or a gene fusion.

The invention belongs to the technical field of bioengineering, and provides a tobacco vascular tissue specific expression promoter pNtERD6L and application thereof, the nucleotide fragment of the promoter pNtERD6L is cloned, a pNtERD6L + GUS expression vector is constructed, agrobacterium tumefaciens-mediated tobacco genetic transformation is carried out, the effect of the promoter pNtERD6L in tobacco plants is evaluated, and the application of the promoter pNtERD6L in the tobacco plants is promoted. Results show that the promoter pNtERD6L can drive a target gene to be specifically expressed in the tobacco vascular tissue, so that adverse effects caused by continuous and efficient expression of an exogenous gene driven by a constitutive promoter in other tissues of the tobacco are avoided.

The application discloses a targeted nutrient source mining method based on beef cattle intestinal type-host gene interaction, and particularly relates to the field of biological information data processing, and is used for solving the problems of lack of molecular mechanism verification and poor targeting of existing nutrient source development. First, based on the microbiome and transcriptome data, the nutrient-related intestinal type is divided, and the co-expression network is constructed to screen the host co-expression specific genes driven by the specific intestinal type. Then, the model of the group metabolite ligand set and the host protein receptor is established, the molecular conformation search and the Gibbs free energy calculation are performed, and the high-activity targeted effect factor is screened based on the physical affinity. Finally, the targeted effect factor is used for traversing matching and quantitative screening of natural raw material liquid chromatography-mass spectrometry data, and a targeted nutrient source recommendation list for the specific intestinal type is generated. The application combines multi-omics correlation analysis and molecular thermodynamic verification, and constructs a precise development closed loop from micro mechanism analysis to macro raw material matching.

In various embodiments methods of utilizing gene drive constructs in asexual organisms such as viruses are provided. In certain embodiments the methods involve transfecting or infecting a cell with a modified DNA virus containing a gene drive construct; and infecting the cell with the target virus where the genome of said target DNA virus is modified by insertion of the gene drive construct into the genome of the target DNA virus and a population of modified target viruses (containing the gene drive construct) is produced.