Root-specific promoter and application thereof

A promoter, specific technology, applied in the application, angiosperm/flowering plant, introduction of foreign genetic material using a vector, etc., can solve the problems that have not been reported at home and abroad, etc.

Active Publication Date: 2013-02-06
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The plant root-specific promoters that have been cloned so far are mainly in Arabidopsis, rice, tobacco, soybean, corn, pine, carrot, and tomato. Most of th...

Method used

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Embodiment Construction

[0044] The present invention will be described in further detail below in conjunction with embodiment.

[0045] Materials and methods

[0046] 1. Materials

[0047] 1.1 Plasmids and strains

[0048] The basic vector pCAMBIA2300, the cloned strain DH5α and the Agrobacterium tumefaciens LBA4404 are all preserved in our laboratory and can be released to the public.

[0049] 1.2 Plant material

[0050] The cultivated peanut (Arachis hypogaea L.) variety Baisha 1016 was provided by the Peanut Research Institute of Shandong Province, and the ecotypes of tobacco (Nicotiana benthamiana) and wild-type Arabidopsis thaliana (Columbia) were preserved in this laboratory and are available to the public. Released publicly.

[0051] 1.3 Reagents

[0052] Ex-Taq high-fidelity polymerase, T4 DNA ligase and restriction endonuclease were purchased from TaKaRa Company; 2×Taq MixPCR was purchased from Beijing Biomed Biotechnology Company; plasmid mini-extraction kit, DNA recovery kit, PCR Produ...

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Abstract

The invention relates to a root-specific promoter and application thereof, belonging to the field of a biological technology. A root-specific expression promoter has a nucleotide sequence shown by SEQ ID NO 1. The root-specific expression promoter is obtained by cloning at the root of peanut; and experiments prove that a gene driven by the promoter is specifically expressed at the roots of arabidopsis thaliana, tobacco and peanut. The root-specific expression promoter has important significance to prevention and control of diseases and pests of roots of plants.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a root-specific expression promoter, its cloning, functional verification and its application. Background technique [0002] Peanut is an important oil crop and economic crop in China. In 2011, my country's peanut planting area was 4.7 million hectares, with an output of 16.2 million tons, ranking first in the world and accounting for 47.9% of the world's peanut production (USDA, World Agricultural Production, United States Department of Agriculture, 2010). As China's soybean area shrinks year by year, the voice of "optimizing the structure of edible oil, developing the peanut industry, and ensuring the strategic security of China's edible oil" is getting louder, and sufficient attention should be paid to peanut pest control and quality improvement. The quality and yield of peanuts are easily affected by soil pests, fungi and bacterial diseases (Vargas Gil S, Haro R, Oddino C, et a1....

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/63A01H5/00
Inventor 张杰耿丽丽段晓红束长龙宋福平彭琦
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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