Epigenetically regulated site-specific nucleases

A nuclease, target site technology, applied in the field of site-specific nucleases regulated by epigenetics
CN110290813APending Publication Date: 2019-09-27THE GENERAL HOSPITAL CORP
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
THE GENERAL HOSPITAL CORP
Publication Date
2019-09-27

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Abstract

Methods and compositions for improving the specificity of genome-editing nucleases (e.g., RNA-guided CRISPR-Cas nucleases or engineered zinc finger nucleases) and customizable DNA-binding domain fusion proteins (e.g., RNA-guided dead-Cas9, RNA-guided dead-Cpf1, or engineered zinc finger arrays fused to transcriptional regulatory domains) for use as research reagents, in gene drives, or as therapeutic agents.
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Description

[0001] priority statement

[0002] This application claims the benefit of U.S. Provisional Application Serial No. 62 / 408,645, filed October 14, 2016. The entire contents of the aforementioned documents are hereby incorporated by reference.

[0003] Federally funded research or development

[0004] This invention was made with US Government support under Grant Nos. DP1 GM105378 and R35GM118158 awarded by the National Institutes of Health. The US Government has certain rights in this invention. technical field

[0005] Described herein are methods for improving genome editing nucleases (such as RNA-guided CRISPR-Cas nucleases or engineered zinc finger nucleases) and customizable DNA-binding domain fusion proteins (such as RNA-guided death cells fused to transcriptional regulatory domains). Cas9, RNA-guided dead Cpf1, or engineered zinc finger arrays) specific methods and compositions for use as research reagents, for gene drives, or as therapeutic agents. Background techniq...

Claims

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