Abiotic stress inducible promoter and application thereof

A promoter and non-biological technology, applied in the field of plant genetic engineering, can solve problems such as death, affect normal growth, increase the burden of plant metabolism, etc., and achieve the effect of enhancing stress resistance and increasing expression level
CN113151273AActive Publication Date: 2021-07-23XINJIANG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
XINJIANG AGRI UNIV
Publication Date
2021-07-23

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Abstract

The invention discloses an abiotic stress inducible promoter and an application thereof. The invention specifically discloses a DNA molecule. The DNA molecule is an inducible promoter PHSPIn9 and an inducible promoter PHSPDel. The promoter is derived from a GhHSP70 gene dominant haplotype promoter in upland cotton, the expression quantity of a GUS gene driven by the promoter under the abiotic stress is detected, and the result shows that both the PHSPIn9 promoter and the PHSPDel promoter can enhance the expression level of the GUS gene under the abiotic stress condition. The relative expression level of the GUS gene driven by the PHSPIn9 promoter is remarkably higher than that of the PHSPDel and CaMV35S. The developed abiotic stress inducible promoter provides an excellent tool for directional expression of genes for genetic engineering breeding of plants for coping with abiotic stress such as high temperature and drought, and has important significance for research and production practice of a plant genetic engineering technology.
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Description

technical field

[0001] The invention belongs to the technical field of plant genetic engineering, and specifically relates to an abiotic stress-inducible promoter and its application, in particular to the development and application of an abiotic stress-inducible promoter PHSPIn9 / PHSPDel derived from cotton. Background technique

[0002] The promoter is a DNA sequence located in the upstream region of the 5' end of the structural gene, which can accurately bind to transcription-related proteins such as RNA polymerase, and has the specificity of transcription initiation. It is an important component of gene expression regulation and controls gene expression. Onset and degree of expression. The plant gene promoter contains a variety of important cis-acting elements, which participate in the regulation of the expression of the corresponding downstream genes at the transcriptional level, making the gene specific in time and space. Therefore, the isolation and functional analysis...

Claims

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