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Pistacia chinensis bunge microsatellite loci, primer and application

A technology of microsatellite sites and microsatellites, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of very few studies and achieve high polymorphism and stability Effect

Active Publication Date: 2017-02-15
HANGZHOU NORMAL UNIV QIANJIANG COLLEGE
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AI Technical Summary

Problems solved by technology

Since Pistacia is an important source of renewable green energy biodiesel and has high ecological and economic value, the research on Pistacia has been paid more and more attention, but the research on Pistacia mainly focuses on physiology, distribution, In terms of energy development and other aspects, there are very few studies on molecular biology level, germplasm resource analysis, population genetic structure, etc.

Method used

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  • Pistacia chinensis bunge microsatellite loci, primer and application
  • Pistacia chinensis bunge microsatellite loci, primer and application
  • Pistacia chinensis bunge microsatellite loci, primer and application

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Embodiment Construction

[0023] The present invention is further analyzed below in conjunction with specific examples.

[0024] 1. Extraction of Genomic DNA from Pistacia chinensis

[0025] Based on the operation of the plant leaf DNA extraction kit of Tiangen Biochemical Technology (Beijing) Co., Ltd., the present invention slightly adjusts the characteristics of Pistacia chinensis to extract the genome of Pistacia chinensis.

[0026] (1) Weigh about 30 mg of Pistacia chinensis leaves that have been dried with silica gel to remove petioles and veins, put them in a 2ml centrifuge tube, soak in liquid nitrogen for 1 min, and grind them fully in a grinder.

[0027] (2) Add 700 μl of preheated GP1 and 2 μl of β-mercaptoethanol to the above centrifuge tube, vortex to mix, and bathe in water at 65°C for 50 min. During the water bath, invert the centrifuge tube several times to mix the sample.

[0028] (3) Add 700 μl of chloroform, mix thoroughly, and centrifuge at 12,000 rpm for 5 minutes.

[0029] (4) T...

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Abstract

The invention is aimed at providing pistacia chinensis bunge microsatellite loci, a primer and application, and provides the twelve pistacia chinensis bunge microsatellite loci, and the primer for designing the microsatellite loci and detecting the population genetic variation of pistacia chinensis bunge. The twelve microsatellite loci are obtained from a pistacia chinensis bunge genome through a magnetic bead enrichment method and a fluorescent label sequencing technology, the specific primer is designed according to flanking sequences of two ends of a microsatellite, the amplification result has high polymorphism and stability and can be used for the fields of population genetic diversity detection, genetic relationship identification, establishment of a genetic linkage map, molecular mark assisted breeding and the like of pistacia chinensis bunge.

Description

technical field [0001] The invention relates to the technical field of molecular biology DNA markers, in particular to microsatellite sites, primers and applications of pistachio chinensis. Background technique [0002] Pistacia chinensis Bunge, belonging to the genus Pistacia chinensis of Anacardiaceae, is a dioecious, deciduous tree. Pistacia chinensis is sun-loving, family-growing, has a certain tolerance to drought and barrenness, and is easy to cultivate. It is widely distributed in China and has been recorded in 23 provinces and municipalities in Northwest, North, East, Central and South China. . Pistacia chinensis not only has high ornamental value, but also has high economic development value. Its wood is tough and corrosion-resistant, and belongs to the first-class construction and furniture materials; its leaves, seeds, bark, etc. can be used as medicine; the oil content of the seeds is as high as 35.05%, Pistacia chinensis oil can not only be used as edible oil,...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 卢剑波丁兆红
Owner HANGZHOU NORMAL UNIV QIANJIANG COLLEGE
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