A kind of immobilized lipase catalyzes the method for preparing biodiesel from pistachio oil

A technology of immobilizing lipase and pistachio oil, which is applied in the fields of immobilized on/in organic carriers, biofuels, chemical industry, etc., can solve problems affecting mass transfer, hindering cross-linking reactions, and blocking enzymes from entering, etc. To achieve the effect of improving the stability of use, low price and long service life

Inactive Publication Date: 2011-11-30
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

One reason is that part of the aldehyde groups are inside the canvas, and intramolecular hydrogen bonds are formed between adjacent hydroxyl groups after encountering water, which blocks the contact between the aldehyde groups and the enzyme protein; the other reason is that after the dense fibers are filled with water and swell, the surface water The electrostatic tension of the membrane blocks the entry of macromolecules such as enzymes and affects mass transfer
Moreover, some adsorbent substances that are not easy to remove in the dry powder enzyme also adhere to the surface of the fiber group, hindering the progress of the crosslinking reaction.

Method used

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  • A kind of immobilized lipase catalyzes the method for preparing biodiesel from pistachio oil
  • A kind of immobilized lipase catalyzes the method for preparing biodiesel from pistachio oil

Examples

Experimental program
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Effect test

Embodiment 1

[0035] The present invention selects natural fiber---ramie as carrier, and three steps of activation of ramie, porcine pancreas lipase liquid preparation and cross-linking are carried out:

[0036](1) Activation of ramie: cut some ramie, boil with 0.5mol / L KOH for 0.5 hour to remove impurities on the surface of ramie, then oxidize with 0.1 mol / L NaI04 solution, take it out after 6 hours and wash it with deionized water , add glycerol to the surface of ramie and let it stand for 40 min to remove unreacted NaIO4, and wash it with deionized water for later use.

[0037] (2) Preparation of enzyme solution: Dissolve a certain amount of porcine pancreatic lipase dry powder in 50mL of phosphate buffer (0.025mol / L, pH 7.5), stir evenly at 4°C, and centrifuge at 10,000r / min for 10 min , and then take the supernatant and store it in the refrigerator for later use.

[0038] (3) Cross-linking: put the activated ramie in step (1) in an Erlenmeyer flask and cross-link with the enzyme s...

Embodiment 2

[0040] The present invention selects natural fiber---ramie as carrier, and three steps of activation of ramie, porcine pancreas lipase liquid preparation and cross-linking are carried out:

[0041] (1) Activation of ramie: cut some ramie, boil with 0.8mol / L KOH for 0.6 hours to remove impurities on the surface of ramie, then oxidize with 0.3 mol / L NaI04 solution, take it out after 8 hours and wash it with deionized water , add glycerol to the surface of ramie and let it stand for 50 min to remove unreacted NaIO4, and wash it with deionized water for later use.

[0042] (2) Preparation of enzyme solution: Dissolve a certain amount of porcine pancreatic lipase dry powder in 50mL of phosphate buffer (0.0.500mol / L, pH 7.5), stir evenly at 4°C, and centrifuge at 10,000r / min for 10 min, and then take the supernatant and store it in the refrigerator for later use.

[0043] (3) Cross-linking: Put the activated ramie in step (1) in a conical flask and cross-link with the enzyme so...

Embodiment 3

[0045] The present invention selects natural fiber---ramie as carrier, and three steps of activation of ramie, porcine pancreas lipase liquid preparation and cross-linking are carried out:

[0046] (1) Activation of ramie: Cut some ramie, boil with 1.0mol / L KOH for 1.0 hour to remove impurities on the surface of ramie, then oxidize with 0.6 mol / L NaI04 solution, take it out after 12 hours and wash it with deionized water, Add glycerol and let it stand for 60 min on the surface of ramie to remove unreacted NaIO4, and wash it with deionized water for later use.

[0047] (2) Preparation of enzyme solution: Dissolve a certain amount of porcine pancreatic lipase dry powder in 50mL of phosphate buffer solution (0.025-0.500mol / L, pH 7.5), stir evenly at a low temperature of 4°C, and dissolve at a speed of 10000r / min Centrifuge for 10 min, then take the supernatant and store it in the refrigerator for later use.

[0048] (3) Cross-linking: put the activated ramie in step (1) in a...

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Abstract

The invention provides a method for preparing biodiesel from immobilized lipase catalyzed pistacia chinensis bunge. The method is characterized in that the biodiesel is prepared by transesterification reaction of immobilized lipase catalyzed pistacia chinensis bunge which takes natural fiber-ramie as a vector and methanol. According to the invention, immobilized lipase can be recovered after esterification reaction and then continuously and repeatedly used thirteen times, and the activity maintaining rate of lipase is larger than 80.0%, thereby improving the utilization of lipase and reducingthe product cost resulting from lipase.

Description

technical field [0001] The invention relates to a preparation method of immobilized lipase. technical background [0002] The covalent binding between the enzyme and the carrier is based on a chemical reaction between reactive amino acid residues on the surface of the enzyme (A) and reactive functional groups on the surface of the carrier (B). In order to achieve effective covalent linkage, relevant functional groups on the carrier or enzyme must be activated in advance. Generally, relevant functional groups on the carrier are activated in advance, and then covalently bonded with the enzyme. In a few cases, such as when it is necessary to control the binding method or the number of covalent bonds, the relevant functional groups on the enzyme molecule are activated in advance, and then covalently bonded to the carrier. [0003] After the enzyme is immobilized, it can not only maintain the catalytic activity of the enzyme but also overcome some shortcomings of the free enzym...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12N11/02
CPCY02E50/13Y02E50/10Y02P20/50
Inventor 刘光斌刘苑秋黄长干胡冬南张露杜天真黄忠邱自兵
Owner JIANGXI AGRICULTURAL UNIVERSITY
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