Preparation method for food-medicine fungus fermentation product rich in folate

A fermentation product and fungus technology, applied in the field of bioengineering, can solve the problems of weakening macrophage phagocytosis, promoting cancer cell growth, adverse effects, etc. Effect

Active Publication Date: 2015-01-07
ZHEJIANG FORESTRY ACAD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Commercially available folic acid is mainly chemically synthesized, but the metabolism of chemically synthesized folic acid and natural folic acid in the human body is differe

Method used

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  • Preparation method for food-medicine fungus fermentation product rich in folate

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 1. Material preparation

[0051] Pectinase (with an enzyme activity of 50,000 U / g) and cellulase (with an enzyme activity of 30,000 U / g) were provided by Guangxi Pangbo Bioengineering Co., Ltd.

[0052] PDA plate medium: potato 200g, glucose 20g and agar 15g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 20min.

[0053] Lactic acid bacteria liquid strains are obtained by static culture of Lactococcus lactis subsp. lactis in liquid MRS medium at 37°C for 15 hours.

[0054] The composition of liquid MRS medium is: peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 20.0g, sodium acetate 5.0g, diammonium hydrogen citrate 2.0g, Tween-801.0ml, dihydrogen phosphate Potassium 2.0g, magnesium sulfate 0.2g, manganese sulfate 0.05g and distilled water 1.0 liter.

[0055] The composition of 1L Phellinus Phellinus liquid seed medium is: glucose 10g / L, yeast powder 4g / L, peptone 3g / L, KH 2 PO 4 1g / L, MgSO 4 0.5g / L and the balance of water, the pH is...

Embodiment 2

[0073] 1. Material preparation

[0074] Pectinase (with an enzyme activity of 40,000 U / g) and cellulase (with an enzyme activity of 20,000 U / g) were provided by Guangxi Pangbo Bioengineering Co., Ltd.

[0075] PDA plate medium: potato 200g, glucose 20g and agar 20g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 20min.

[0076] Lactic acid bacteria liquid bacterial classification is with embodiment 1.

[0077] The composition of 1L Phellinus Phellinus liquid seed medium is: glucose 15g / L, yeast powder 4g / L, peptone 3g / L, KH 2 PO 4 1g / L, MgSO 4 0.5g / L and the balance of water, the pH is natural.

[0078] 1L Ganoderma lucidum liquid seed medium consists of: glucose 20g / L, yeast powder 6g / L, peptone 6g / L, KH 2 PO 4 1g / L, MgSO 4 0.5g / L and the balance of water, the pH is natural.

[0079] a. Preparation of enzymatic hydrolyzate of cabbage leaves, including: washing cabbage leaves, vacuum freeze-drying, weighing 100g and adding 1000mL water, grinding into a...

Embodiment 3

[0093] 1. Material preparation

[0094] Pectinase (with an enzyme activity of 30,000 U / g) and cellulase (with an enzyme activity of 30,000 U / g) were provided by Guangxi Pangbo Bioengineering Co., Ltd.

[0095] PDA plate culture medium: with embodiment 1.

[0096] Lactic acid bacteria liquid strains are obtained by static culture of Lactococcus lactis subsp. lactis in liquid MRS medium at 35°C for 10 hours. The liquid MRS medium is the same as in Example 1.

[0097] The composition of 1L Phellinus Phellinus liquid seed medium is: glucose 20g / L, yeast powder 4g / L, peptone 3g / L, KH 2 PO 4 1g / L, MgSO 4 0.5g / L and the balance of water, the pH is natural.

[0098] 1L Ganoderma lucidum liquid seed medium consists of: glucose 25g / L, yeast powder 6g / L, peptone 6g / L, KH 2 PO 4 1g / L, MgSO 4 0.5g / L and the balance of water, the pH is natural.

[0099] a. Preparation of cabbage leaf enzymatic hydrolyzate, including: after cleaning the cabbage leaves, vacuum freeze-drying, weighing...

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Abstract

The invention discloses a preparation method of a food-medicine fungus fermentation product rich in folate. The preparation method comprises inoculating activated phellinus igniarius and lucid ganoderma strains in a liquid seed medium added with a pistacia chinensis bunge extract, culturing to obtain liquid strains of phellinus igniarius and lucid ganoderma; inoculating the liquid phellinus igniarius strain into a solid medium added with carrot and barley, culturing, drying and crushing a cultured product to obtain a solid culture I; then inoculating the liquid lucid ganoderma strains into a liquid fermentation medium added with the solid culture I and cabbage zymolyte, culturing for a certain while, then inoculating a lactobacillus strain, and culturing to obtain a fermentation product. According to the characteristics of fruits and vegetables rich in folate, like carrots and cabbages, and the fermentation characteristics of the food-medicine phellinus igniarius and lucid ganoderma, two-stage fermentation of a food-medicine fungus fermentation process and then a lactobacillus fermentation process is adopted, so that the content of the folate in the final fermentation product is increased, and the preparation method is a production method having an extensive industrial prospect.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a preparation method of a fungal fermentation product rich in folic acid. Background technique [0002] Phellinus igniarius belongs to Basidiomycotina, Phellinus class, Rustiaceae, Pinifera. Phellinus extract has significant effects in the clinical application of inhibiting cancer cell metastasis and preventing cancer recurrence after surgery. It is currently an edible fungus with the highest efficiency among internationally recognized biological cancer treatment agents. [0003] Due to the particularity and complexity of the physiological state and the constraints of the external environment, Phellinus seldom forms fruiting bodies in nature, especially it takes many years to form usable fruiting bodies. Moreover, artificial cultivation is extremely difficult, the cultivation conditions are harsh, and the growth cycle is as long as 3-4 years, which is difficult to meet ...

Claims

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Application Information

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IPC IPC(8): A23L1/28A61K36/074A23L31/00
CPCA23L31/00
Inventor 程俊文贺亮胡传久魏海龙付立忠李海波
Owner ZHEJIANG FORESTRY ACAD
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