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Tissue culture and rapid propagation process of Chinese Pistache

A technology for tissue culture and rapid propagation of Pistacia chinensis, applied in horticulture, cultivation, soilless cultivation, etc., can solve the problems of easy pollution of stem segment tissue culture, difficulty in obtaining sterile seedlings, high browning rate, etc., and achieve reproductive efficiency Low, optimized medium formula, low chance of variation

Active Publication Date: 2012-11-14
SICHUAN COLORLINK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The germination rate of seeds is low, and it is difficult to obtain sterile seedlings; while the tissue culture of stem segments is easy to be polluted, the rate of browning is high, the germination is slow, and new shoots are easy to die, etc., it is difficult to obtain sterile seedlings

Method used

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  • Tissue culture and rapid propagation process of Chinese Pistache
  • Tissue culture and rapid propagation process of Chinese Pistache
  • Tissue culture and rapid propagation process of Chinese Pistache

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The process of tissue culture and rapid propagation of Pistacia chinensis includes the following steps:

[0028] A. Obtain aseptic seedlings. Collect mature seeds of Pistacia chinensis in the field, first digest them with 50% sulfuric acid for 12, then remove the testa; then sterilize with 60% alcohol for 4 minutes, sterilize with mercury for 15 minutes, and wash with sterile water for 5 minutes. Times; then use pliers to clamp the seed shell on the ultra-clean workbench, and inoculate the seeds on MS basic medium without any hormones. The MS medium does not add any plant growth regulators (plant hormones), Carry out 3d dark culture for 3-7 days, then light / dark culture for 16hr / 8hr, the temperature of the culture room is 25℃; Observation records, after 1 month, some seeds can be seen to germinate and grow into complete plants to obtain sterile seedlings;

[0029] B. Pistacia chinensis tissue culture and rapid propagation. Aseptic seedlings are used for tissue culture and ra...

Embodiment 2

[0037] The process of tissue culture and rapid propagation of Pistacia chinensis includes the following steps:

[0038] A. Obtain aseptic seedlings. Collect mature seeds of Pistacia chinensis in the field, first digest them with 70% sulfuric acid for 12, then remove the testa; then sterilize with 80% alcohol for 2 minutes, sterilize with mercury for 25 minutes, and wash with sterile water 6 Times; then use pliers to clamp the seed shell on the ultra-clean workbench, and inoculate the seeds on MS basic medium without any hormones. The MS medium does not add any plant growth regulators (plant hormones), Carry out 3d dark culture for 3-7 days, then light / dark culture for 16hr / 8hr, the temperature of the culture room is 28℃; observation records, after 2 months, some seeds can be seen to germinate and grow into complete plants to obtain sterile seedlings;

[0039] B. Pistacia chinensis tissue culture and rapid propagation. Aseptic seedlings are used for tissue culture and rapid propagat...

Embodiment 3

[0048] The process of tissue culture and rapid propagation of Pistacia chinensis includes the following steps:

[0049] A. Obtain aseptic seedlings. Collect mature seeds of Pistacia chinensis in the field, first digest them with 60% sulfuric acid for 18 hours, then remove the testa; then sterilize with 70% alcohol for 3 minutes, sterilize with mercury for 20 minutes, and wash with sterile water 5 Times; then use pliers to clamp the seed shell on the ultra-clean workbench, and inoculate the seeds on MS basic medium without any hormones. The MS medium does not add any plant growth regulators (plant hormones), Carry out 3d dark culture for 3-7 days, then light / dark culture for 16hr / 8hr, the temperature of the culture room is 26℃; Observation records, one and a half months later, some seeds can be seen to germinate and grow into complete plants, and sterile seedlings are obtained;

[0050] B. Pistacia chinensis tissue culture and rapid propagation. Aseptic seedlings are used for tissue...

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Abstract

The invention discloses a tissue culture and rapid propagation process of Chinese Pistache. The tissue culture and rapid propagation process of Chinese Pistache includes the steps of A, collecting ripe seeds of Chinese Pistache, and removing episperm by sulfuric acid digestion; sterilizing; inoculating the seeds to MS (Murashige and Skoog) minimal medium in which no hormone is added; B, cutting aseptic seedlings into stem segments with buds, removing part of leaves, inoculating to rooting medium or inoculating to enrichment medium prior to transferring to the rooting medium; and C, exercisingand transplanting the tissue culture seedlings. The tissue culture and rapid propagation process of Chinese Pistache has the advantages that the tissue culture method is modified, a medium formula isoptimized, and aseptic seedlings can be obtained easily and can be propagated rapidly and grow healthy and vigorous; mass high-quality test-tube seedlings can be obtained rapidly from the Chinese Pistache which is difficult or slow to propagate under natural conditions and can be applied to culture and production, and variation rate of offspring of the Chinese Pistache obtained by the process is low.

Description

Technical field [0001] The invention relates to the technical field of plant tissue culture and rapid propagation, in particular to a process for tissue culture and rapid propagation of Pistacia chinensis. Background technique [0002] Pistacia chinensis (Pistacia chinensis), also known as Kaishu etc., belongs to the genus Pistacia of the Anacardiaceae family. It is an excellent timber and ornamental deciduous tree. It is widely distributed in my country, from Hebei and Shandong in the north, Guangdong and Guangxi in the south, Taiwan in the east, and Sichuan and Yunnan in the west. There are wild and cultivated pistacia trees in Hebei, Henan, Shanxi, Shaanxi and other provinces. Pistacia chinensis has a wide range of uses. Its seed oil content is 42.5%, and its oil yield is 20.0%~30.0%. It has been designated by the State Forestry Administration as one of the key forestry biomass energy tree species to be developed during the "Eleventh Five-Year Plan" period. First, it has extr...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00
Inventor 吴燚马建华冯爱云
Owner SICHUAN COLORLINK CO LTD
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