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SSR molecular marker primer based on transcriptome data of azalea as well as screening method and application of SSR molecular marker primer

A technology of molecular markers and screening methods, which is applied in the fields of biochemical equipment and methods, recombinant DNA technology, and the determination/inspection of microorganisms, which can solve the problem of indeterminate SSR.

Inactive Publication Date: 2016-09-28
HUANGGANG NORMAL UNIV
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Problems solved by technology

However, the use of this technology must be supported by the corresponding SSR sequence. At present, SSR markers can be divided into genomic SSR (gSSR) and transcriptomic SSR (EST-SSR). Since the genome of Rhododendron has not been sequenced or the sequencing data has not yet been published, and other It has not been determined whether the SSR developed by the genome of homologous species can be used in common with it, so there is no related report on the development of SSR markers using Rhododendron genome SSR or transcriptome data in the prior art

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  • SSR molecular marker primer based on transcriptome data of azalea as well as screening method and application of SSR molecular marker primer
  • SSR molecular marker primer based on transcriptome data of azalea as well as screening method and application of SSR molecular marker primer
  • SSR molecular marker primer based on transcriptome data of azalea as well as screening method and application of SSR molecular marker primer

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Embodiment Construction

[0030] The screening method of the SSR molecular marker primer based on Rhododendron transcriptome data in the embodiment of the present invention comprises the following steps:

[0031] (1) extracting total RNA;

[0032] (2) Constructing a rhododendron transcriptome sequencing library, and then performing high-throughput sequencing on the transcriptome sequencing library;

[0033] (3) filtering and assembling the data of the transcriptome sequencing library to obtain a Unigene database;

[0034] (4) carry out SSR site search to the sequence of described Unigene database, screen out the sequence that meets SSR primer design;

[0035] (5) Perform batch SSR primer design on the sequence of the Unigene database containing the SSR site to obtain SSR molecular marker primers.

[0036] In the screening method of SSR molecular marker primers based on rhododendron transcriptome data in the embodiment of the present invention, the RNA in the step (1) is selected from the early bud ma...

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Abstract

The invention discloses an SSR molecular marker primer based on transcriptome data development and specific application of the SSR molecular marker primer, belonging to the technical field of biology. The primer is obtained based on development of a transcriptome sequence. By virtue of primer designs of carrying out batch screening on lots of sequencing data based on transcriptome sequencing to obtain an SSR sequence and carrying out SSR marking, the obstacles that the SSR molecular markers of the azalea are few, the development efficiency is low, and the like are overcome. The validity of the SSR primer is verified by virtue of different varieties of azaleas, and foundation is laid for the research of genetic diversity of the azaleas, the constitution of genetic linkage maps, the assistant breeding of the molecular marker, and the like.

Description

technical field [0001] The invention relates to the field of molecular marker technology development and application. More specifically, it involves the development and application of SSR molecular marker primers based on Rhododendron transcriptome data, which are suitable for the identification of genetic diversity, genetic linkage map construction, QTL mapping, and molecular marker-assisted breeding of Rhododendron. Background technique [0002] Rhododendron simsii Planch is an evergreen or deciduous shrub of the Rhododendron family (Ericaceae) and is an important landscaping and ornamental plant. Rhododendron generally blooms in spring, with 2-6 flowers per cluster, funnel-shaped corolla, red, light red, apricot red, snow green, white, etc., with luxuriant and bright colors. It is a famous flower plant with high ornamental value. The whole plant of this species is used medicinally to promote qi and blood circulation, tonify deficiency, treat internal injury cough, kidney...

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
CPCC12Q1/6811C12Q1/6895C12Q2600/156C12Q2535/122C12Q2537/165
Inventor 王书珍方元平金卫斌向福项俊程华
Owner HUANGGANG NORMAL UNIV
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