2results about How to "Variation Stable" patented technology

The application provides a molecular marker and identification method for identifying genuine producing areas and non-genuine producing areas of Astragalus membranaceus. The molecular marker contains a nucleotide sequence with a polymorphism of A / T at the 131st base of the sequence shown in SEQ ID NO: 1 of the 5th exon of Am02G035490 of the Astragalus membranaceus genome. The application also provides a method for identifying the genuine producing areas of Astragalus membranaceus by using the SNP molecular marker, which provides technical support for the precise traceability, stable quality and healthy and orderly development of the Astragalus membranaceus industry of the genuine producing areas of Astragalus membranaceus.

The invention relates to an insertion and deletion marker NC037647.18674876 for identifying the high-yield character of bee colony royal jelly of western bees and application of the insertion and deletion marker NC037647.18674876 The method comprises the following steps: randomly collecting adult worker bee individuals from a to-be-detected western bee colony, extracting genome DNA (deoxyribonucleic acid) as a template, and carrying out PCR (polymerase chain reaction) amplification reaction by using a primer of NC037647.18674876 to obtain a PCR reaction product; carrying out agarose gel electrophoresis detection on the obtained PCR reaction product, and sequencing the PCR reaction liquid with a target band; nC037647.18674876 site genotyping is carried out according to a sequencing result, the genotype is a homozygous insertion type II, a homozygous deletion type DD or a heterozygous type ID, the I allele shows 14bp insertion, and the D allele shows 14bp deletion; the allele frequency of the NC037647.18674876 site of the to-be-detected western bee colony is calculated, and whether the to-be-detected western bee colony is the royal jelly high-yield bee colony or not is determined. The method can accurately, quickly and scientifically identify the royal jelly high-yield character of the western bee colony, can greatly shorten the breeding period of the royal jelly high-yield bees, and accelerates the breeding speed of the bees.