InDel molecular marker primer of Modilong white gourds and application of InDel molecular marker primer

Abstract

The invention discloses an InDel molecular marker primer of Modilong white gourds and an application of the InDel molecular marker primer. The primer comprises primer pairs corresponding to 7 point sites, and the nucleotide sequences are shown as SEQID NO.1-SEQ ID NO.14. When the Modilong white gourds are subjected to hybrid purity identification, firstly genome DNA of seeds of samples to be detected is extracted to be used as a template, then the primer is used for performing PCR amplification, and finally amplification products are detected through electrophoresis. Banding patterns of parents and hybrids are compared, the proportion of the number of real hybrids to the total number of seeds of strips which can be observed, namely the hybridization purity, is obtained. The InDel marker provided by the invention can realize effective distinguishing of the parents from the hybrids, the identification cost is low, the efficiency is high, and the coincidence degree of an identification result and a field identification result reaches 99% or above.

Description

technical field [0001] The invention relates to the technical field of molecular markers, in particular to a set of InDel molecular marker primers and applications thereof. Background technique [0002] Molecular markers are a new form of genetic markers developed after morphological markers, cell markers and biochemical markers. It is based on mutations in proteins and nucleic acid molecules, and detects biological genetic structures and their variations. There are many defects and problems in the identification of enzymes and proteins by electrophoresis, so they are widely used in genetic breeding, gene positioning and diversity analysis. Molecular marker methods commonly used in plant genetic research include restriction fragment length polymorphism (RFLP), random amplified polymorphism (RAPD) and simple repeat sequence marker (SSR), but the above-mentioned techniques have the following defects: RFLP requires The participation of radioactive isotopes is more complicated;...

AI Technical Summary

Problems solved by technology

Molecular marker methods commonly used in plant genetic research include restriction fragment length polymorphism (RFLP), random amplified polymorphism (RAPD) and simple repeat sequence marker (SSR), but the above-mentioned techniques have the following defects: RFLP requires The participation of radioactive isotopes, the technology is more complicated; the repeatability of RAPD experiment is poor, and the reliability of the result is low; compared with the former two, SSR is simpler, cheaper, and has better repeatability, but some amplification bands are unclear and false, etc. Technical defects such as alleles and null alleles lead to errors in genotyping and data analysis of SSR markers

[0004] At present, the field identification method is basically adopted for the identification of the hybrid purity of winter melon commercial varieties in my country, that is, to judge whether the seeds are hybrids according to the morphological performance of the crops. The number of morphological characteristics used to identify the purity of hybrids is limited, and most of the morphological characteristics are easily affected by the environment, which cannot meet the production needs of the year

[0005] In fact, there are relatively few studies on the development and utilization of wax gourd molecular markers in my country, and there are no related reports on the application of wax gourd InDel markers.

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