Two indel markers closely linked to the yellow-white peel of cucumber and their applications

A yellow-white, 1.indel technology, applied in the fields of genetic engineering and molecular biology, can solve the problems of weak gene localization research on the yellow-white peel of cucumber, no InDel markers, etc., and achieves low experimental cost, simple and clear detection, and specificity. strong effect

Active Publication Date: 2021-01-26
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on the mapping of cucumber yellow-white peel genes is relatively weak, and there is no research report on the use of InDel markers in cucumber tender peel color-assisted selection breeding

Method used

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  • Two indel markers closely linked to the yellow-white peel of cucumber and their applications
  • Two indel markers closely linked to the yellow-white peel of cucumber and their applications
  • Two indel markers closely linked to the yellow-white peel of cucumber and their applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Construction of genetic segregation populations

[0027] 1. F 2 Group construction

[0028] YN (female parent), yellow-white peel; the stem is thick and strong, and when the internodes grow to 12-14 nodes, the top is capped with a bunch of female flowers and stops growing, the leaves are green, the fruit is short, the fruit handle is short, and the peel is yellow-white.

[0029] JIN5-508 (male parent), green peel; it is a vine variety with tall plants and long internodes. In each progeny, a plant with a longer fruit stem is selected, and the inbred line with a longer fruit stem is obtained by selfing to the 8th generation when the traits are no longer separated.

[0030] This implementation case uses these two parents to configure the hybrid combination F 1 , F 1 F 2 group. In 551 F 2 In the population, the peel color of cucumber fruits developed 9 days after pollination was measured. The peel color was measured visually and with a colorimeter. Segregation r...

Embodiment 2

[0041] Example 2: Detection of InDel Markers on Cucumber Inbred Lines of Cucumber Peel Color

[0042] (1) Select 20 cucumber inbred lines with known cucumber peel color, and 10 green peels (ACUO57, KP2, AMO22, MM97, OFBAT17, JIN5, EP6411OFBAT07, 649, D8). 10 yellow-white peels (Bai Guifei, CAP15, Dabai, Ocean White Cucumber, CAP14, CAP11, YN, Pingwang Milkmelon, Baixiu, FRANCH).

[0043] (2) Genomic DNA of the above-mentioned 20 varieties and their parents were extracted respectively, and PCR amplification was performed using Chr3InDel-fs01 and Chr3InDel-fs02 markers. The results of ChrINdel-fs01 in 10 green peel cucumber inbred lines and 10 yellow-white peel cucumber inbred lines showed that the genotype was consistent with the phenotype. (Such as Figure 5 ) ChrINdel-fs02 in 10 green peel cucumber inbred lines and 10 yellow-white peel cucumber inbred lines, the genotype is consistent with the phenotype. (Such as Figure 6 )

[0044] (3) The results of agarose gel electr...

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Abstract

The invention discloses two InDel markers closely linked with the yellow-white pericarp of cucumber, the two InDel markers are respectively Chr3InDel-fs01 and Chr3InDel-fs02, the nucleotide sequence of the Chr3InDel-fs01 is TGTCTTTATCCTATAGA, and the Chr3InDel-fs01 The nucleotide sequence of fs02 is CGAGAGA. The invention also discloses the application of the InDel marker in molecular marker-assisted breeding of cucumber yellow-white pericarp. The specific method is: (1) to F 2 Each strain of the population was genotyped using the obtained molecular markers. (2) Using F in the above (1) 2 According to the identification results of the population genotype, the individual plants are classified, and the phenotype results are identified and verified. The InDel molecular marker of the present invention is stable in variation, simple and clear in detection, does not require enzymatic digestion, and has low experiment cost, improves the efficiency of cucumber peel color breeding, and accelerates the process of cucumber peel color breeding.

Description

technical field [0001] The invention belongs to the fields of genetic engineering and molecular biology, and in particular relates to an InDel marker closely linked with cucumber yellow and white cucumber genes. Background technique [0002] Cucumber (Cucumis sativus L.) is a plant of the genus Cucurbitaceae, which originated in the warm and humid tropical rainforest area at the southern foot of the Himalayas. Cucumber is one of the main vegetable crops in my country, with the largest planting area and the widest planting range in China. With the improvement of people's quality of life, people have higher and higher requirements for product quality, especially its appearance quality. Cucumber skin color, fruit length, fruit stem length, etc. are important traits that affect the commerciality of cucumbers. Quality breeding has become one of the key points of cucumber breeding. It is of great practical significance to study the genetic law and molecular markers of cucumber t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 陈学好刘倩雅许学文齐晓花徐强
Owner YANGZHOU UNIV
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