The indel molecular marker gyindel3 closely linked to the all-female trait of Zucchini and its application
A technology of molecular markers and zucchini, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the limitations of research progress on sex determination genetics of zucchini, have not yet obtained Indel molecular markers, zucchini To solve problems such as narrow genetic basis, achieve the effect of speeding up the process of molecular assisted breeding, simple and clear detection, and stable variation
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Embodiment 1
[0035] First, the all-female genetic analysis zucchini
[0036] 1, F2 populations constructed
[0037] (1) parental A36, dark green rind, short cylindrical, less star point, white flesh, whole homozygous female; male SX, green rind, long cylindrical, star points, melon meat, green, ordinary flowers ( figure 1 . This configuration using the two parent hybrid combinations F1, F1 selfed F2 population, reciprocal cross F1 plants showed ordinary type of pattern.
[0038] 2, genetic analysis
[0039] 2018 autumn, F2 population, 235 for the general performance of the hermaphrodite type, the performance for the whole 73 female; the spring of 2019, 144 showed common type of hermaphrodite, 52 performance for the whole female. Ordinary hermaphrodite and female plants full of the type of separation ratio, chi-square test 3: 1. Zucchini infer gynoecious is controlled by a single recessive gene, gynoecious ordinary hermaphrodite type of recessive.
[0040] Second, the whole zucchini female gene...
Embodiment 2
[0053] Application of Molecular Breeding Indel marker-assisted selection in the molecule gyIndel3
[0054] Using primers gyIndel3-F and gyIndel3-R for "broad qua Guangdong" selfing F2 generation plant 13 for genotyping gynoecious; weak parts 10 and the female species zucchini product (section V black hair, even a small part of the seed, green and green zucchini, zucchini Guanhua No. 6, emerald green zucchini, zucchini Tsui Po, Kwai excellent No. 1, No. 2 Gui excellent zucchini, zucchini crown asterisk 2, gem F1 zucchini , numbered 1 to 10) for genotyping analysis.
[0055] (1) DNA extraction zucchini, on the "broad qua Guangdong" selfing gynoecious 13 and 10 parts of a commercial plant species in the F2 generation zucchini leaves DNA extraction was performed, as follows:
[0056] 1) Take the blade 2.0mL centrifuge tube was placed in a dry powder, the dry powder in an amount just cover the bottom of the centrifuge tube is appropriate;
[0057] 2) preheating added 800μL 2% CTAB buff...
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