Indel molecular marker gyIndel3 closely linked with all-female characters of chieh-qua and application of Indel molecular marker gyIndel3

A molecular marker, a technology of zucchini, which can be used in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., which can solve the problems that limit the progress of research on sex determination of zucchini, the narrow genetic basis of zucchini, and the lack of access to Indel molecules. Marking and other issues to achieve the effect of speeding up the process of molecular-assisted breeding, simple and clear detection, and stable variation

Active Publication Date: 2021-05-07
INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on the sex type of Zucchini mainly focuses on physiological research and preliminary genetic rules, and there are few reports on molecular mechanism research.
In addition, due to the narrow genetic basis of zucchini, the genes related to sex determination have not been cloned, and the Indel molecular markers closely linked to them have not been obtained, which greatly limits the progress of genetic research on sex determination of zucchini.

Method used

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  • Indel molecular marker gyIndel3 closely linked with all-female characters of chieh-qua and application of Indel molecular marker gyIndel3
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  • Indel molecular marker gyIndel3 closely linked with all-female characters of chieh-qua and application of Indel molecular marker gyIndel3

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Genetic Analysis of All-female Zucchini

[0036] 1. Construction of F2 population

[0037] (1) Female parent A36, dark green rind, short cylindrical shape, few star spots, white flesh, homozygous all-female type; male parent SX, green rind, medium-long cylindrical shape, many star spots, melon Flesh green, common pattern ( figure 1 ). Using these two parents to configure the hybrid combination F1, F1 self-crosses to generate F2 populations, and the sex types of the reciprocal F1 plants all show common flower types.

[0038] 2. Genetic analysis

[0039] In the autumn of 2018, in the F2 population, 235 plants were common hermaphrodites, and 73 plants were all females; in the spring of 2019, 144 plants were common monoecious plants, and 52 plants were all females. The segregation ratio of common hermaphroditic and all-female sex types was 3:1 by Chi-square test. Therefore, it can be inferred that all females of Z. melon are controlled by a single recessive single g...

Embodiment 2

[0053] Application of Indel Molecular Marker gyIndel3 in Molecular Assisted Selection Breeding

[0054] Using primers gyIndel3-F and gyIndel3-R, the genotypes of 13 all-female plants in the self-crossed F2 generation of "Yueguang Zhegua" were detected; Small seed link, green zucchini, Guanhua No. 6 zucchini, emerald emerald zucchini, Cuibao zucchini, Guiyou No. 1, Guiyou No. 2 zucchini, Guanxing No. 2 zucchini, Baoyu F1 zucchini , numbered 1-10) for genotype detection and analysis.

[0055] (1) Extraction of Zythias melon DNA, DNA extraction was performed on 13 all-female plants and 10 copies of leaves of commercial variety Zythias melon in the self-bred F2 generation of "Yueguang Zhegua", and the specific operations were as follows:

[0056] 1) Take the leaf dry powder and place it in a 2.0mL centrifuge tube, the amount of dry powder should just cover the bottom of the centrifuge tube;

[0057] 2) Add 800 μL 2% CTAB preheating buffer;

[0058] 3) Water bath at 65°C for 1.0...

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Abstract

The invention discloses an Indel molecular marker gyIndel3 closely linked with all-female characters of chieh-qua, a primer and application of the Indel molecular marker gyIndel3 in the aspect of all-female breeding of the chieh-qua. The Indel marker disclosed by the invention is stable in mutation, simple and clear in detection, does not need enzyme digestion, is relatively low in cost, is conducive to the breeding efficiency of the all-female characters, and accelerates the breeding process of the female line of the chieh-qua; and the marker disclosed by the invention is closely linked with the all-female characters of the chieh-qua, lays a foundation for cloning and molecular mechanism research of all-female genes, and accelerates the molecular auxiliary breeding process of the female lines of the chieh-qua.

Description

technical field [0001] The invention belongs to the technical field of molecular markers, and in particular relates to an Indel molecular marker gyIndel3 closely linked with the all-female trait of zucchini and its application. Background technique [0002] Chieh-qua How (Benincasa hispida Cogn.var.Chieh-qua How), also known as hairy melon, hairy zucchini, is a variety of wax gourd species of Cucurbitaceae wax gourd. Nutrients such as diacids are the main vegetable crops exported in South China (He et al., 2007). [0003] There are many sex types of Zygia gourd, including all female plants, strong female plants, and monoecious plants, etc. Female flowers appear early, at low nodes, in large numbers, and evenly distributed, which are the basis for early maturity, high yield and stable yield of Zygia gourd (Robinson , 2000; He et al., 2007). At present, all females or strong females are mostly used for dominant breeding in the production of zucchini. However, due to the com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6895C12Q2600/13C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 王敏彭庆务何晓明刘文睿江彪谢大森林毓娥梁肇均陈林闫晋强
Owner INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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