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Polymorphic molecular marker for identifying Xinluzhong series cotton varieties, and application of polymorphic molecular marker

A polymorphic marker and molecular marker technology, which is used in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc.

Active Publication Date: 2020-04-21
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most studies on the fingerprints of upland cotton varieties in Xinjiang focus on extra-early and early-maturing cotton varieties, and the construction of fingerprints for early-medium-maturing upland cotton varieties needs to be improved

Method used

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  • Polymorphic molecular marker for identifying Xinluzhong series cotton varieties, and application of polymorphic molecular marker
  • Polymorphic molecular marker for identifying Xinluzhong series cotton varieties, and application of polymorphic molecular marker
  • Polymorphic molecular marker for identifying Xinluzhong series cotton varieties, and application of polymorphic molecular marker

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Experimental program
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Embodiment 1

[0117] 1. Materials

[0118] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified, and the reagents and other materials used are all commercially available products unless otherwise specified.

[0119] 2. Method

[0120] The present invention utilizes the core primers with high polymorphism, good stability and strong identification ability screened out, and takes 80 copies of Xinluzhong series cotton varieties as test materials (Table 1) to construct the DNA fingerprints of Xinluzhong series cotton varieties, Molecular markers that can be used to identify 80 Xinluzhong series cotton varieties were obtained. A total of 14 characteristic primers (Table 2) and combination primers (Table 3) of 66 varieties in Xinlu were obtained. Using at least 19 pairs of core primers can completely distinguish 80 Xinluzhong series cotton varieties, and construct the decimal digital fingerprint codes of 80 Xinluzhong series cotto...

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Abstract

The invention relates to a polymorphic molecular marker for identifying Xinluzhong series cotton varieties, and application of the polymorphic molecular marker, and belongs to the technical field of molecular detection. 29 pairs of screened polymorphism identification primers are adopted to realize fingerprint construction and molecular identification of 80 Xinluzhong series cotton varieties, andthe 29 pairs of primers have the advantages of high polymorphism, good stability, simple operation, high accuracy, good universality and the like; according to the invention, only 19 pairs of core primers are needed to identify 80 Xinluzhong series cotton varieties.

Description

technical field [0001] The invention belongs to the technical field of molecular detection, and in particular relates to a polymorphic molecular marker for identifying Xinluzhong series cotton varieties and its application. Background technique [0002] Cotton is an important economic crop rich in fiber. Xinjiang is the main production area of ​​cotton in my country, with about 50% of the planting area contributing nearly 3 / 4 of the output, which plays a pivotal role in ensuring the safety of cotton production in my country. In recent years, with the rapid development of the cotton industry, the phenomenon of "multiple, chaotic, mixed, and miscellaneous" in the cotton seed market has become increasingly serious, and problems such as homogeneity of cotton varieties and branding of varieties have become prominent, seriously affecting the healthy development of the cotton industry. In addition, the repeated use of backbone parents and the application of transgenic technology in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 薛飞李婧慧金彦龙孙杰刘峰张新宇王静
Owner SHIHEZI UNIVERSITY
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