Molecular marker of single fruit weight major quantitative trait loci (QTL) of August red pyrus L. fruits and application of molecular marker

A technology of molecular markers and single fruit weight, which is applied in the field of molecular genetic breeding, can solve problems such as lack of development, and achieve the effects of improved accuracy, good application value, and improved accuracy and efficiency

Inactive Publication Date: 2011-08-17
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0004] At present, the research on QTL mapping of quantitative traits of pear is still in its infancy. The existing researches are mainly aimed at some diseases

Method used

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  • Molecular marker of single fruit weight major quantitative trait loci (QTL) of August red pyrus L. fruits and application of molecular marker
  • Molecular marker of single fruit weight major quantitative trait loci (QTL) of August red pyrus L. fruits and application of molecular marker
  • Molecular marker of single fruit weight major quantitative trait loci (QTL) of August red pyrus L. fruits and application of molecular marker

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Experimental program
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Effect test

Embodiment 1

[0028] a) Using the hybrid combination of two pear varieties 'August Red' and 'Dangshansuli' in my country, 97 F 1 group.

[0029] b) Analyze the two parents and their F 1 Population, statistical analysis of genetic types of polymorphic sites between parents in offspring populations. A total of 732 polymorphic marker loci, including 23 SSRs, S gene loci, 226 SRAPs, and 482 AFLPs, were finally obtained, and then linkage analysis was performed on them using Jionmap3.0 mapping software to construct a table containing Genetic linkage map of 240 polymorphic markers in 'August Red' pear.

[0030] Using SSR, SRAP, and AFLP molecular marker methods, the 'August Red' and 'Dangshan Suli' and their offspring were analyzed, and the genetic types of the polymorphic sites between the parents were statistically analyzed in the offspring population.

[0031]For the experimental procedure of SSR labeling, refer to the method of Yomamoto T. et al. (Euphytica, 2002, 124:129-137); for the expe...

Embodiment 2

[0058] Using the screened molecular markers linked to the main effect QTL for single fruit weight of ‘Augusthong’, the 97 F strains of ‘Augusthong’ and ‘Dangshansuli’ were compared 1 Preliminary testing was carried out on the hybrid progenies to test the practical value of this method in molecular marker-assisted selection of pear fruit single fruit weight. Carry out PCR reaction with the genomic DNA of these 97 offspring and AFLP selective amplification primer combination E-ACA / M-CAC, the operation process of reaction is with the AFLP molecular labeling method among the embodiment 1, and PCR amplification result is in non-denaturing polymer Acrylamide gel electrophoresis (results such as figure 2 ), after electrophoresis, determine whether there is a corresponding mark according to the presence or absence of the EACAMCAC-2000m band. If there is a mark, it means that the fruit weight of the plant is high, and if it does not exist, it means low. Verify with molecular marker t...

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Abstract

The invention belongs to the field of genetic breeding, and provides a molecular marker of single fruit weight major quantitative trait loci (QTL) of August red pyrus L. fruits and application of the molecular marker. The genetic linkage map of August red is constructed by using SRAP (sequence-related amplified polymorphism), SSR (simple sequence repeats) and AFLP (amplified fragment length polymorphism), phenotype identification on single fruit weight of pyrus L. is combined, and an interval mapping method is used to analyze linkage groups, thus major QTL is detected on the seventh linkage group of August red and can explain 30.9% of characteristics of single fruit weight. The point which is the nearest to the major QTL site Pfw-1 is marked to be EACAMCAC-2000m, and the nearest distance is 1.019cM. The obtained titratable acid major QTL molecular marker has significant theoretical and practical means for quickening genetic improvement process of pyrus L. varieties and improving breeding selection efficiency.

Description

technical field [0001] The invention belongs to the field of molecular genetic breeding. The invention relates to molecular markers and applications of the main effect QTL of 'August Red' pear fruit single fruit weight, which can be used for early molecular assisted selection of pear fruit single fruit weight traits to improve breeding efficiency. Background technique [0002] Pear (Pyrus L.) is native to my country and is the third largest fruit tree species in my country. Pears are deeply loved by consumers because of their high nutritional value, sweetness and juiciness. Single fruit weight is not only an important factor affecting the appearance quality and commerciality of pear fruit, but also one of the important factors that constitute the yield and economic benefits of pear varieties. Therefore, the size of pear fruit single fruit weight is very important for pear varieties. Breeding varieties with ideal fruit weight has become one of the important goals of pear bre...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 张瑞萍张绍铃李秀根吴俊杨健
Owner NANJING AGRICULTURAL UNIVERSITY
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