Molecular marker for single fruit weight main-effect quantitative trait loci (QTL) of Dangshansu pear fruit and application thereof

A Dangshansu pear and molecular marker technology, which is applied in the field of molecular genetics and breeding, can solve the problems of positioning and molecular markers are not developed, and achieve good application value, improve accuracy and efficiency, and improve the effect of accuracy.

Active Publication Date: 2011-08-03
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0004] At present, the research on QTL mapping of quantitative traits of pear is still in its infancy. The existing researches are mainly on some

Method used

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  • Molecular marker for single fruit weight main-effect quantitative trait loci (QTL) of Dangshansu pear fruit and application thereof
  • Molecular marker for single fruit weight main-effect quantitative trait loci (QTL) of Dangshansu pear fruit and application thereof
  • Molecular marker for single fruit weight main-effect quantitative trait loci (QTL) of Dangshansu pear fruit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] a) Using the hybrid combination of two pear varieties 'August Hong' and 'Dangshansu pear' in my country, 97 F 1 group.

[0031] b) Analyze the two parents and their F 1Population, statistical analysis of genetic types of polymorphic sites between parents in offspring populations. A total of 732 polymorphic marker loci, including 23 SSRs, S gene loci, 226 SRAPs, and 482 AFLPs, were finally obtained, and then linkage analysis was performed on them using Jionmap3.0 mapping software to construct a table containing Genetic linkage map of 'Dangshansuli' with 240 polymorphic markers.

[0032] Using SSR, SRAP, and AFLP molecular marker methods, the 'August Red' pear and 'Dangshansu pear' and their offspring were analyzed, and the genetic types of the polymorphic sites between the parents were statistically analyzed in the offspring population.

[0033] For the experimental procedure of SSR labeling, refer to the method of Yomamoto T. et al. (Euphytica, 2002, 124:129-137); fo...

Embodiment 2

[0045] Using the screened molecular markers linked to the main effect QTL for single fruit weight of ‘Dangshansu pear’, the 97 strains of ‘August Hong’ and ‘Dangshansu pear’ 1 Preliminary testing was carried out on the hybrid progenies to test the practical value of this method in molecular marker-assisted selection of pear fruit single fruit weight. Use the genomic DNA of the 97 offspring and SRAP primers fe3 and em4 to carry out PCR reactions, and the PCR amplification system for SRAP analysis is a total volume of 20ul: which contains 0.2mmol L -1 dNTPs, 0.3 μmol L -1 Upstream and downstream primers, 2.0mmol L -1 MgCl 2 , 1 unit of rTaq enzyme, 50ng of DNA template, 1×Buffer. The PCR reaction program was: 94°C for 3min; 35 cycles: 94°C for 40s, 55°C for 50s, 72°C for 1min; 72°C for 10min, 10°C for 10min. The results of PCR amplification were electrophoresed on 8% non-denaturing polyacrylamide gel (results such as figure 2 ), after electrophoresis, determine whether the...

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Abstract

The invention belongs to the field of inheritance breeding, and relates to a molecular marker for a single fruit weight main-effect quantitative trait loci (QTL) of Dangshansu pear fruit and application thereof. A genetic linkage map of Dangshansu pear is constructed by utilizing sequence-related amplified polymorphism (SRAP), simple sequence repeats (SSR) and amplified fragment length polymorphism (AFLP), phenotypic identification on the single fruit weight of the fruit is combined, a group is analyzed through interval mapping, the presence of the main-effect QTL is detected on the 13th linkage group of the Dangshansu pear, and the 48.8 percent of single fruit weight can be interpreted. A molecule closest to the main-effect QTL Pfw-1 is marked as fe3em4-242p* and the distance is 0cM. The obtained molecular marker for the single fruit weight main-effect QTL has important theoretical and practical guidance significance for quickening the genetic improvement progress of pear varieties and improving breeding selection efficiency.

Description

technical field [0001] The invention belongs to the field of molecular genetic breeding, and provides molecular markers and applications of the main effect QTL of single fruit weight of 'Dangshansu pear', which can be used for early molecular assisted selection of pear fruit single fruit weight traits to improve breeding efficiency. Background technique [0002] Pear (Pyrus L.) is native to my country and is the third largest fruit tree species in my country. Pears are deeply loved by consumers because of their high nutritional value, sweetness and juiciness. The size of each pear fruit, which is commonly referred to as single fruit weight, is one of the important indicators of pear fruit appearance quality and commercial properties, and is also an important factor that constitutes the yield and economic benefits of pear varieties. Therefore, the size of pear fruit single fruit weight has the greatest impact on pear varieties. important. Breeding varieties with ideal fruit...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12N15/10C12Q1/68
Inventor 吴俊张绍铃张瑞萍吴华清陶书田齐开杰
Owner NANJING AGRICULTURAL UNIVERSITY
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