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Hermaphrodite laver dihaploid colony building method

A double haploid and hermaphroditic technology, applied in botany equipment and methods, plant genetic improvement, horticulture, etc., can solve the problem of difficulty in heterozygous filaments

Inactive Publication Date: 2016-11-09
SHANGHAI OCEAN UNIV
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Problems solved by technology

[0004] The difficulty in the process of constructing the genetic map of Porphyra zebra is hybridization, because Porphyra zebra is a hermaphroditic species. During the hybridization process, self-fertilization and heterozygous fertilization are carried out at the same time, and the probability of self-fertilization is much higher than that of heterozygous fertilization. Difficulty in identifying heterozygous filaments from allogeneic fertilization

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  • Hermaphrodite laver dihaploid colony building method
  • Hermaphrodite laver dihaploid colony building method
  • Hermaphrodite laver dihaploid colony building method

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Embodiment

[0036] 1.1 Selection and cultivation of the thallus (gametophyte) of the hybrid parent of Porphyra variegata

[0037] Porphyra zebra as the hybrid parent should have obvious morphological markers, and generally choose algal bodies with different colors that can be clearly distinguished by the naked eye. In this plan, the wild color PY-LS ( figure 1 , left) and the red mutant line PY-HT ( figure 1 , right) as an example. In addition, while considering the polymorphic differences and fertility between parents, we should try our best to select materials with relatively distant kinship, large genetic differences, and strong polymorphism of molecular markers as hybrid parents, so as to facilitate the development of genes with good traits. positioning and cloning.

[0038] Weigh 100 mg of parental filaments (spores) with a wet weight, put them on a sterilized plastic backing plate, chop them into short algae segments with a sterilized single-sided blade, and transfer them to a gla...

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Abstract

The invention discloses a hermaphrodite laver dihaploid colony building method. The method comprises taking hermaphrodite laver thalluses (gametophytes) with obvious color difference as cross parents, carrying out mixed cultivation on father and mother thalluses with antheridiums, collecting carpospores, selecting the carpospores with germ tubes, carrying out single culture to obtain protonema (sporophore), carrying out induction to obtain conchospore of the protonema, carrying out culture to obtain macroscopic F1 thalluses, wherein if 90% or more of the thalluses contain amphiphilic color chimeras, the thalluses are used for building of a dihaploid colony, separating all color blocks of the four-color block chimera, acquiring monospores or single isolated cells of each one of the color blocks, carrying out culture to obtain macroscopic thalluses, carrying out single thallus culture and autofertilization to obtain pure thallus of each one of color blocks of the four-color block chimera and building a dihaploid colony. The method solves problems of hermaphrodite laver dihaploid colony building and lays the foundation of genetic linkage map construction.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for forming a hermaphroditic laver double haploid population. Background technique [0002] Pyropia yezoensis in hermaphroditic laver is a large edible seaweed with high nutritional value. It is widely cultivated in the area north of the Yangtze River in my country, creating an economic benefit of about 5 billion yuan every year. At the same time, the large Pyropia yezoensis Large-scale cultivation can transfer and fix a large amount of carbon (to prevent ocean acidification) and elements such as nitrogen and phosphorus (to prevent eutrophication), which is of great significance to maintain the ecological balance of the coastal waters. Porphyra zebra has been cultivated for more than 40 years, and has formed a complete industrial system of seedling raising, cultivation, processing and sales. On the contrary, basic theoretical research on its genetics has been relatively insuf...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01G33/02
CPCA01G33/00A01H1/02
Inventor 黄林彬严兴洪
Owner SHANGHAI OCEAN UNIV
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